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EC number: 420-640-8 | CAS number: 138495-42-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 996
- Report date:
- 1996
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Remarks:
- The study was conducted according to guideline in effect at time of study conduct.
- GLP compliance:
- yes
Test material
- Reference substance name:
- -
- EC Number:
- 420-640-8
- EC Name:
- -
- Cas Number:
- 138495-42-8
- Molecular formula:
- C5H2F10
- IUPAC Name:
- (3R,4R)-1,1,1,2,2,3,4,5,5,5-decafluoropentane; (3S,4S)-1,1,1,2,2,3,4,5,5,5-decafluoropentane
- Details on test material:
- - Purity: 99.7%
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: Samples were collected from each replicate test vessel after 0 and 48 hours (final samples were collected before 48 hours if complete mortality occurred in a test vessel)
- Sampling method: GC equipped with flame ionization detector
- Sample storage conditions before analysis: Not reported
Test solutions
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION: A 1000 mg/L stock solution was formulated by combining test substance and dilution water in a glass jar. The test substance was added below the water surface with a syringe. The jar was filled to capacity to eliminate head space and tightly capped. The stock solutions were placed on an orbital shaker adjusted to approximately 150 rpm and incubated at approximately 30°C for 24 hours. Solutions were then incubated at 20±1°C for 48 hours without shaking. Dilution water used to formulate test media and for the control was shaken and incubated in the same manner as the stock solutions. Following the 72-hour incubation, the stock solution was carefully removed from the glass jar, and an effort was made to avoid collecting insoluble test substance. The stock solution was used immediately to formulate test media. The dilution water was carbon-filtered, deionized water. The dilution water was stored in polyethylene tanks, where it was aerated and recirculated through particle filters, activated carbon, and an ultraviolet sterilizer.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM:
- Strain: Daphnia magna
- Age at study initiation: juvenile
- Feeding during test: None
ACCLIMATION
- Acclimation period: Not reported
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- hardness ranged from 160-180 mg/L
- Test temperature:
- Temperature ranged from 19.3-20.6°C
- pH:
- pH ranged from 7.6-8.6
- Dissolved oxygen:
- Dissolved oxygen ranged from 8.2-9.3 mg/L
- Nominal and measured concentrations:
- Nominal concentrations: 0, 8.1, 14, 23, 38, 64, and 106 mg/L
Measured concentrations (initial): 0, 8.59, 13.9, 21.8, 37.1, 60.4, and 98.2 mg/L
Measured concentrations (final): 0, 4.34, 7.02, 10.2, 18.4, 27.7, and 47.9 mg/L (samples from nominal 38, 64, and 106 mg/L were collected at 24 hours) - Details on test conditions:
- TEST SYSTEM
- Test vessel: 40 mL (nominal) glass vials containing 42 mL of test solution; because of the volatile nature of the test substance, test vessels were sealed and were not opened during the exposure period unless complete mortality occurred.
- No. of organisms per vessel: 4
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Conductivity: Conductivity ranged from 570-590 µmhos/cm
OTHER TEST CONDITIONS
- Adjustment of pH: Not reported
- Photoperiod: 16 hour light and 8 hour dark, with a 15-minute transition period between dark and light
- Light intensity: 85 foot-candles
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Mortality and immobilization at 0, 24, and 48 hours
TEST CONCENTRATIONS
- Range finding study: A method trial study was conducted under static conditions using a stock solution prepared in dimethylformamide with control and 5 test concentrations (0.0016, 0.016, 0.16, 1.6, and 16 mg/L). At the end of 48 hours, there was 100% survival at all concentrations. A second method trial study was conducted under static conditions using a stock solution prepared in dilution water with a control and 2 test concentrations (160 and 1600 mg/L). At the end of 48 hours, there was 100% survival in the control, 65% survival at 160 mg/L, and 25% survival at 1600 mg/L. Daphnids were seen stuck to insoluble beads of test substance in test vessels. A third method trial study was conducted under static conditions using a stock solution prepared in dilution water and stirred for 24 hours with a control and 1 test substance (1600 mg/L). At the end of 48 hours, there was 100% survival in the control and 1600 mg/L. A rangefinding test was conducted under static conditions with a solvent control and 2 test concentrations (8 and 80 mg/L) prepared using a stock solution prepared in dimethylformamide. At the end of 48 hours, there was 100% survival in all test vessels. A second range-finding test was conducted under static conditions with a control and 3 test concentrations (0.93, 9.5, and 110 mg/L). At the end of 48 hours there was 0% survival at 110 mg/L and 100% survival at all other concentrations.
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 10.6 mg/L
- Nominal / measured:
- meas. (initial)
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% confidence interval of 8.59-13.9 mg/L
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 11.7 mg/L
- Nominal / measured:
- meas. (initial)
- Basis for effect:
- mortality
- Remarks on result:
- other: 95% confidence interval of 8.59-13.9 mg/L
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- < 8.59 mg/L
- Nominal / measured:
- meas. (initial)
- Details on results:
- - Behavioural abnormalities: Immobilization was observed at 24 hours (21.8 mg/L) and 48 hours (≥8.59 mg/L)
- Mortality of control: 0% - Reported statistics and error estimates:
- Results of the toxicity test were analyzed using standard statistical techniques. The binomial/nonlinear interpolation method was used to calculate LC50 values using the number of dead test organisms and EC50 values using the number of dead and affected organisms. Calculations were performed using the initial measured concentrations of the test substance. The NOEC is the highest concentration of test substance that allowed at least 90% survival and did not cause sublethal effects.
Any other information on results incl. tables
Table 1: Mortality
|
||||
Initial Measured Concentration (mg/L) |
Replicate |
Number Alive |
||
0 hr |
24 hr |
48 hr |
||
0 |
1 |
5 |
5 |
5 |
|
2 |
5 |
5 |
5 |
|
3 |
5 |
5 |
5 |
|
4 |
5 |
5 |
5 |
8.59 |
1 |
5 |
5 |
5 |
|
2 |
5 |
5 |
5 |
|
3 |
5 |
5 |
5 |
|
4 |
5 |
5 |
5 |
13.9 |
1 |
5 |
5 |
0 |
|
2 |
5 |
5 |
1 |
|
3 |
5 |
5 |
0 |
|
4 |
5 |
5 |
2 |
21.8 |
1 |
5 |
2 |
0 |
|
2 |
5 |
5 |
0 |
|
3 |
5 |
5 |
0 |
|
4 |
5 |
4 |
0 |
37.1 |
1 |
5 |
0 |
0 |
|
2 |
5 |
0 |
0 |
|
3 |
5 |
0 |
0 |
|
4 |
5 |
0 |
0 |
60.4 |
1 |
5 |
0 |
0 |
|
2 |
5 |
0 |
0 |
|
3 |
5 |
0 |
0 |
|
4 |
5 |
0 |
0 |
98.2 |
1 |
5 |
0 |
0 |
|
2 |
5 |
0 |
0 |
|
3 |
5 |
0 |
0 |
|
4 |
5 |
0 |
0 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The study and the conclusions which are drawn from it fulfil the quality criteria (validity, reliability, repeatability).
48-hour EC50 = 10.6 mg/L
48-hour LC50 = 11.7 mg/L
48-hour NOEC <8.59 mg/L - Executive summary:
The acute toxicity test to daphnids was performed under static conditions with 6 nominal concentrations (8.1, 14, 23, 38, 64, and 106 mg/L) of test substance and a dilution water control at 20±1°C. The test substance was formulated into a supersaturated stock solution (1000 mg/L) in dilution water, mixed in a sealed container for 24 hours at 30°C, and allowed to equilibrate to test temperature for 48 hours. Water was carefully removed from the mixing vessel to avoid insoluble material and used immediately to initiate the toxicity test. Initial measured concentrations were ND, 8.59, 13.9, 21.8, 37.1, 60.4, and 98.2 mg/L. Because of the volatile nature of the test substance, the test was performed in sealed 40 mL (nominal) test vessels that contained 42 mL of test solution to eliminate head space. Vessels were not opened during the exposure period unless complete mortality occurred. All animals were in good condition at the beginning of the study and 100% of the control daphnids survived the toxicity test. Exposure of daphnids to the test substance resulted in a 48-hour LC50 of 11.7 mg/L, with a 95% confidence interval of 8.59-13.9 mg/L and 48-hour EC50 of 10.6 mg/L, with a 95% confidence interval of 8.59-13.9 mg/L. The 48-hour NOEC was <8.59 mg/L.
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