Benzenamine, 4-fluoro-N-(1-methylethyl)-

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: inherent biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

07 Mar - 05 Apr 2005

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2005

Report date:

2005

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)

Version / remarks:

1992-07-17

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

-

EC Number:

448-100-7

EC Name:

-

Cas Number:

70441-63-3

Molecular formula:

C9H12FN

IUPAC Name:

4-fluoro-N-(propan-2-yl)aniline

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic (adaptation not specified)

Details on inoculum:

-Source of inoculum/activated sludge: A mixed population of aquatic microorganisms (activated sludge) was obtained on 07 March 2005 from the aeration tank of sewage plant Leverkusen-Bürrig, Germany
-Preparation of inoculum/activated sludge for exposure: The activated sludge was washed twice and separated by centrifugation
- Concentration of sludge: 0.4 g/l

Duration of test (contact time):

28 d

Initial test substance concentration

Initial test substance concentration 1

Initial conc.:

2 g/L

Based on:

test mat.

Initial test substance concentration 2

Initial conc.:

100 mg/L

Based on:

DOC

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

DOC removal

Details on study design:

TEST CONDITIONS
- Composition of medium: mineral medium
- Test temperature: 20-25 °C
- Continuous darkness: yes


TEST SYSTEM
- Culturing apparatus: 2 L glass vessels
- Number of culture flasks/concentration: 2 vessels and one concentration


SAMPLING
- Sampling frequency: DOC degradation was measured at 0h, 3h, 1, 7, 14, 21, 27, and 28 days



CONTROL AND BLANK SYSTEM
- Inoculum blank: 2 vessels
- Toxicity control: 1 vessel
- Reference control: 2 vessels

Reference substance

Reference substance:

benzoic acid, sodium salt

Results and discussion

% Degradation

Parameter:

% degradation (test mat. analysis)

Value:

18

Sampling time:

28 d

Remarks on result:

other:

Remarks:

see details on results

BOD5 / COD results

Results with reference substance:

Points of degradation plot (reference substance):
99 % degradation after 28 d

Any other information on results incl. tables

Table 1. Results DOC Degradation given as mean of mean results

Test vessel	% Degradation after n days
	3h	1 d	7 d	14 d	21 d	27 d	28 d
Test compound	0	1	0	7	12	18	18
Reference compound	0	94	100	99	100	100	99
Toxicity control	0	41	48	50	54	57	57

 

Table 2. Validity criteria OECD 302 B

Criterion from the guideline	Outcome	Validity criterion fulfilled
The reference compound has been degraded≥70 % within 14 days.	99 % degradation	Yes
No toxicity has been observed in the toxicity coontrol.	57% after 28 d	Yes
The DOC content oft the blank control should be ≤10 mg/l in the beginning.	< 5 mg/l	Yes

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Remarks:

See Table 2 in any other information on results incl. tables

Interpretation of results:

not readily biodegradable