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EC number: 214-772-3 | CAS number: 1193-24-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1995-11-08 to 1996-01-04
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study performed according to guideline and under GLP without deviations.
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-A (Determination of the "Ready" Biodegradability - Dissolved Organic Carbon (DOC) Die-Away Test)
- Deviations:
- no
- GLP compliance:
- yes
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Sewage treatment plant Marl-Ost, Germany
- Storage length: < 1d
- Concentration of sludge: suspended solids: 24.2 mg/L, dry weight: 4.0 g/L - Duration of test (contact time):
- 28 d
- Initial conc.:
- ca. 17.5 mg/L
- Based on:
- DOC
- Parameter followed for biodegradation estimation:
- DOC removal
- Details on study design:
- TEST CONDITIONS
- Composition of medium: 85 mg/L KH2PO4, 217.5 mg/L K2HPO4, 334 mg/L Na2HPO4*2H2O, 5 mg/L (NH4)Cl, 22.5 mg/L MgSO4*7H2O, 27.5 mg/L CaCl2, 0.25 mg/L FeCl3*6H2O (medium compliant with EU Method C.4 and OECD Guideline 301)
- Test temperature: 21.5-22.0°C
- pH: 7.3-7.4
- pH adjusted: no
TEST SYSTEM
- Culturing apparatus: 2L Erlenmeyer flasks
- Number of culture flasks/concentration: 2
- Measuring equipment: DOC analyzer (Shimadzu)
- Test performed in closed vessels: yes; vessels were loosley closed by aluminium foil
SAMPLING
- Sampling frequency: at 0 and 3 hours, and at days 7, 14, 21, 27, and 28.
- Sampling method: Samples were acidified and degassed to remove inorganic carbon and subsequently centrifuged
CONTROL AND BLANK SYSTEM
- Inoculum blank: 2 flasks with inoculum but without test item
- Positive control: 2 flasks with positive control substance (sodium benzoate) - Reference substance:
- benzoic acid, sodium salt
- Parameter:
- % degradation (DOC removal)
- Value:
- 10
- Sampling time:
- 7 d
- Parameter:
- % degradation (DOC removal)
- Value:
- 31
- Sampling time:
- 14 d
- Parameter:
- % degradation (DOC removal)
- Value:
- 67
- Sampling time:
- 21 d
- Parameter:
- % degradation (DOC removal)
- Value:
- 75
- Sampling time:
- 27 d
- Parameter:
- % degradation (DOC removal)
- Value:
- 78
- Sampling time:
- 28 d
- Details on results:
- Degradation of the test item was 78% within 28 days.
The pass level for 70% degradation within 10 days was not reached, as the duration from 10% degradation to 70% degradation was 16 days (test day 7 to 23).
The reference substance sodium benzoate showed 100% degradation within 14 days. - Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable, but failing 10-day window
- Conclusions:
- The test item 4,6-dihydroxypyrimidine (CAS 1193-24-4) showed 78% degradation over a period of 28 days in the DOC die away test according to EU Method C.4-A. The pass level of 70% degradation within a 10d period was not reached.
- Executive summary:
The ready biodegradability of 4,6-dihydroxypyrimidine (CAS 1193-24-4) was assessed using the DOC-die-away test according to EU Method C.4 -A without deviations under GLP. Briefly, the test item was incubated for 28 days in the dark with a suspension of activated sludge from a domestic sewage treatment plant and DOC concentration was quantified weekly. Negative (activated sludge without test item) and positive (sodium benzoate with activated sludge) controls were run in parallel. The test item 4,6-dihadroxypyrimidine showed 78% DOC removal (degradation) within the test period of 28 days. The pass level of 70% degradation within a 10d period was not reached. The reference substance sodium benzoate showed 100% degradation within 14 days, proving the suitability of the test system. Taking together, 4.6-dihydroxypyrimidine is biodegradable but as the requested 10d window was failed, a classification as "readily biodegradable" is not indicated.
Reference
Table 1: Degradation of the test item 4.6-dihydroxypyrimidine in the DOC-Die-away test. Results are means of 2 determinations per flask.
Test |
flask |
DOC concentration in [mg/L] |
||||||
at 0h |
at 3h |
at 7d |
at 14 d |
at 21d |
at 27d |
at 28d |
||
Test item |
1 |
17.17 |
16.79 |
15.30 |
12.62 |
6.51 |
4.65 |
4.65 |
2 |
17.82 |
16.91 |
15.50 |
10.91 |
5.59 |
3.90 |
3.70 |
|
Blank |
1 |
0.54 |
0.54 |
0.68 |
0.36 |
0.74 |
0.38 |
0.75 |
2 |
0.24 |
0.26 |
0.48 |
0.39 |
0.61 |
0.10 |
0.29 |
|
Mean |
0.39 |
0.40 |
0.58 |
0.38 |
0.68 |
0.24 |
0.52 |
Table 2: Degradation of the test item 4.6-dihydroxypyrimidine in the DOC-Die-away test: % degradation
flask |
% DOC removal |
||||||
at 0h |
at 3h |
at 7d |
at 14 d |
at 21d |
at 27d |
at 28d |
|
1 |
0.00 |
0.00 |
10.19 |
25.32 |
64.43 |
73.09 |
74.80 |
2 |
0.00 |
0.00 |
9.63 |
36.22 |
70.26 |
77.83 |
80.74 |
Mean |
0.00 |
0.00 |
9.91 |
30.77 |
67.35 |
75.46 |
77.77 |
Rounded |
0 |
0 |
10 |
31 |
67 |
75 |
78 |
Description of key information
78% DOC removal (degradation) in 28 days, 70% degradation within a 10d period was not reached, EU Method C.4 (DOC Die-Away test), Diefenbach 1996
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable but failing 10-day window
- Type of water:
- freshwater
Additional information
The substance is biodegradable but as the requested 10d window was failed, a classification as "readily biodegradable" is not indicated. The results are sufficiently detailed and unambiguous as to be reliable for risk assessment, classification and labeling.
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