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EC number: 930-389-0 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2008-10-07 to 2008-11-06
- Reliability:
- 1 (reliable without restriction)
Data source
Referenceopen allclose all
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 009
- Report date:
- 2009
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 008
- Report date:
- 2009
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Version / remarks:
- 2006
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- signed 2007-12-07
Test material
Reference
- Name:
- Unnamed
- Type:
- Constituent
- Type:
- Constituent
- Details on test material:
- - Name of test material (as cited in study report): Symrose
- Substance type: technical product
- Physical state: colourless to pale yellow liquid
- Storage condition of test material: ambient temp., dark and dry
No further details are given.
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
No further test substance was used.
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- The determination of the active substance in water samples was performed with a suitable analytical method (GC-MS).
- Concentrations: 0.102, 0.256, 0.64, 1.6, 4, 10 and 25 mg/L
- Sampling method: The concentration course of Symrose was verified in test medium by analysing the contents in the samples over the whole test period in intervals of 24 hours. Samples were taken after initiation of the test and thereafter in 1 day intervals until the end of the test after 72 hours at the concentration levels of 1.6, 4, 10 and 25 mg/L and control.
Due to the fact that the EC0 was 4 mg/L and the EC100 10 mg/L it was decided not determining the actual analytical concentration of the lower test concentrations.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- no
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Common name: Desmodesmus subspicatus
- Strain: SAG 86.81
- Source (laboratory, culture collection): cultures of the "Pflanzenphysiologisches Institute der Universität Göttingen"
- Method of cultivation: The algae are grown in semi-continuously in the laboratory in aerated liquid cultures under permanent illumination. Old medium was periodically replaced by fresh mineral solution in order to keep the algae in an exponential growth state. Culture conditions were as followed: 22-25°C, 1000mL screw cap bottles as culture flasks, CO2 supply ba aeration and illumination with OSRAM light tubes (approx 7000 lux).
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Post exposure observation period:
- none
Test conditions
- Hardness:
- no data
- Test temperature:
- 23-24°C
- pH:
- t = 0: 7.42 - 7.72
t = 72: 8.24 - 8.95 - Dissolved oxygen:
- no data
- Salinity:
- NH4Cl 15 mg/L
MgCl2 x 6 H2O: 12 mg/L
CaCl2 x 2 H2O: 18 mg/L
MgSO4 x 7 H2O: 15 mg/L
KH2PO4: 1.6 mg/L
FeCl3 x 6 H2O: 0.08 mg/L
Na2EDTA x 2H2O: 0.1 mg/L
H3BO3: 0.185 mg/L
MnCl2 x 4 H2O: 0.415 mg/L
ZnCl2: 3 µg/L
CoCl2 x 6H2O: 1.5 µg/L
CuCl2 x 2H2O: 0.01 µg/L
Na2MoO4 x 2H2O: 7 µg/L
NaHCO3: 50 mg/L - Nominal and measured concentrations:
- nominal concentrations: 1.6, 4, 10 and 25 mg/L (spaced by the factor 2.5)
measured concentrations: 1.2, 2.2, 9.1 and 26.1 mg/L (based on geometric mean) - Details on test conditions:
- TEST SYSTEM
- Test vessel: Erlenmeyer flasks with aluminium caps and two baffles
- Material, size, headspace, fill volume: glass, 500 mL flask with a final volume of approximately 167 mL in each test vessel
- Initial cells density: 0.5 x 10^4
- Control end cells density: 120.52 x 10^4
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
TEST MEDIUM / WATER PARAMETERS
- Standard medium used: yes
OTHER TEST CONDITIONS
- Adjustment of pH: yes
- Light intensity and quality: 400W, approx. 7000 lux (continuous illumination)
EFFECT PARAMETERS MEASURED
- Determination of cell concentrations: fluorescence detection after 24, 48 and 72 hours of growth
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2.5
- Range finding study
- Test concentrations: 0, 0.01, 0.1, 1, 10 and 100 mg/L
- Results used to determine the conditions for the definitive study: inhibitory effects were observed from 1 to 100 mg/L - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 6.07 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 4.61 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 6.4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 4.85 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 2.58 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 1.96 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- ca. 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- ca. 7.59 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- > 4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- > 3.03 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Details on results:
- for individual results see attached tables
- Results with reference substance (positive control):
- Results after 3 days test duration with K2Cr2O7 (Batch No.: K29220264 141).
- EC50 cell number: 0.49 mg/L
- EC50 growth rate: 1.56 mg/L
- EC50 biomass integral: 0.41 mg/L
- EC50 yield: 0.41 mg/L
- LOEC: 0.256 mg/L
- NOEC: 0.1024 mg/L - Reported statistics and error estimates:
- To estimate the LOEC, and hence the NOEC, ANOVA was used to calculate the mean average specific growth rate for each test concentration. The resulting mean for each test concentration was compared with the control mean (all controls pooled, including control) using an the comparison method of Dunnett’s test (DUNNETT, 1964). Before pooling data for the control, they were statistically tested to see that they are not significantly different, using two-tailed t-test.
A test for normality of the data was done by calculating the Shapiro-Wilk’s statistic.
Any other information on results incl. tables
The validation of the analytical method was performed in accordance with SANCO/3029/99 rev. 4 (11/07/00):
Linearity: The analytical system gave linear response in the calibration range of 0.1 to 20 μg/mL. The correlation coefficient (r) of the calibration curves was 0.9999.
LOQ: The LOQ of the analytical method was fixed at nominal 0.8 mg/L of the test item and checked by means of accuracy
The mean recovery rate (fortification level 0.8 and 100 mg/L n = 5) was between 97 and 99% with a RSD between 4.8 and 6.2%. The response of blank values of test medium control samples was lower than 30 % of LOQ.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- see executive summary
- Conclusions:
- Despite the loss of test material during the test, there is no drastic change in the toxicological assessment. Symrose was found to be toxic to algae after 72 hours (1 mg/L < EC50 < 10mg/L).
Symrose was found to be toxic to Daphnia magna after 48 hours. The EC50 (48 hours) was 7.86 (7.37 – 8.38) mg/L. The NOEC after 48 hours was 1.40 mg/L. The LOEC after 48 hours was 3.06 mg/L. - Executive summary:
The test fulfils the validity criteria in accordance with OECD Guideline for Testing of Chemicals No. 201, since
(i) cell numbers, measured in the controls between 0 h and 72 h, were found to increase by a factor of 241 which exceeds the threshold of 16, this corresponds to a growth rate of 1.83 · d-1
(ii) the coefficient of variation of average growth in replicate control cultures did not exceed 7 % for the whole test period
(iii) The mean coefficient of variation for the section-by-section specific growth rates (days 1-2 and 2-3) in the control cultures did not exceed 35% (e.g. 24 % and 7 %), but the mean coefficient of variation for the section-by-section specific growth rate for days 1-2 exceeded 35 % (105 %).
However, the section-specific growth rate of replicate number 1 (out of 6 replicates) is statistically an outlier (smaller than 2 standard deviations below the mean) in this section (Days 0-1) ONLY, but not in the other sections.The coefficient of variation for the section-by-section specific growth rates (days 0-1) is calculated to be 13% without replicate 1 (n=5). Considering that fact and that the growth rates for other sections comply with the guideline criteria it can be concluded that the vailidity criteria of the study are fulfilled (see attachment "raw data").
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