Endo-8-isopropyl-8-azabicyclo[3.2.1]octan-3-ol

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

19 April - 26 July 2001

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2001

Report date:

2001

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Endo-8-isopropyl-8-azabicyclo[3.2.1]octan-3-ol

EC Number:

222-314-9

EC Name:

Endo-8-isopropyl-8-azabicyclo[3.2.1]octan-3-ol

Cas Number:

3423-25-4

Molecular formula:

C10H19NO

IUPAC Name:

Endo-8-isopropyl-8-azabicyclo[3.2.1]octan-3-ol

Test material form:

solid: particulate/powder

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic (adaptation not specified)

Details on inoculum:

Species: activated sludge, micro organisms from a domestic waste water treatment plant.
Origin: supplied by sewage plant Groß-Zimmern, Germany
Coditioning:
- The activated sludge used for this study was washed by centrifugation and the supernatant liquid phase was decanted. The solid material was resuspended in tap water and again centrifuged. This precedure was repeated twice.
- An aliquot of the final sludge suspension was weighed, dried and the ratio of wet sludge to its dry weight was determined. Based on this ratio, calculated aliquots of washed sludge suspension, corresponding to 3.9 g dry material per litre were mixed with test water and then aerated until use. Before use the sludge was filtered through cotton wooI.

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

2.12 mg/L

Based on:

ThOD

Remarks:

corresponding to 2.12 mg/L test item, respectively a ThODNH4 of about 5.41 mg 02/L

Reference substance

Reference substance:

aniline

Results and discussion

% Degradation

Parameter:

other: ThODN(NH4) and ThOD(NO3)

Value:

0

Sampling time:

28 d

Details on results:

Under the test conditions the percentage biodegradation of NIsopropylnortropin was determined to be 0 % after 28 days of incubation,
based on ThODNNH4 and based on ThODN03. The test item can therefore be considered to be not ready biodegradable.

BOD5 / COD results

Results with reference substance:

The reference item Aniline was sufficiently degraded to a mean of 72 % after 14 days, and to a mean of 97 % after 28 days of incubation.

Any other information on results incl. tables

Table 1. Dissolved oxygen concentrations at different time intervals during the exposure period
of28 days

Treatment	Conc. [mg/L]	flask No.	mg O2 after n days of exposure
			0	1	5	7	12	14	22	28
Test Item	2.12	1a	8.4	8.4	8.4	8.3	8.3	8.2	8.2	7.8
		1b	8.4	8.5	8.5	8.4	8.3	8.3	8.1	7.8
		mean	8.4	8.45	8.45	8.35	8.3	8.25	8.15	7.8
Procedure Control (Aniline)	2.02	2a	8.4	8.5	7.0	5.6	5.2	4.7	4.0	3.0
		2b	8.4	8.5	7.4	5.6	5.2	4.8	4.2	3.2
		mean	8.4	8.5	7.2	5.6	5.2	4.75	4.1	3.1
Inoculum Control	_	3a	8.4	8.5	8.5	8.3	8.4	8.2	8.1	7.7
		3b	8.4	8.5	8.4	8.4	8.3	8.3	8	7.9
		mean	8.4	8.5	8.45	8.35	8.35	8.25	8.05	7.8
Toxicity Control	TI: 2.04 Aniline: 2.14	4a	8.4	n.d	n.d	6.8	n.d	4.8	4.2	3.4
		4b	8.4	n.d	n.d	7	n.d	4.6	4.1	3.2
		mean	8.4	n.d	n.d	6.9	n.d	4.7	4.15	3.3

n.d.: not determined
TI: test item

 

Table 2. Oxygen depletion at different time intervals during the exposure period of28 days

Treatment	Conc. [mg/L]	flask No.	O2 depletion (mg/L) after n days of exposure
			1	5	7	12	14	22	28
Test Item	2.12	1a	0.10	0.05	0.05	0.05	0.05	-0.15	0.00
		1b	0.00	-0.05	-0.05	0.05	-0.05	-0.05	0.00
Procedure Control (Aniline)	2.02	2a	0.00	1.45	2.75	3.15	3.55	4.05	4.80
		2b	0.00	1.05	2.75	3.15	3.45	3.85	4.60
Inoculum Control	_	mean	-0.10	-0.05	0.05	0.05	0.15	0.35	0.60
Toxicity Control	TI: 2.04 Aniline: 2.14	4a	n.d	n.d	1.55	n.d	3.45	3.85	4.40
		4b	n.d	n.d	1.35	n.d	3.65	3.95	4.60

n.d.: not determined
TI: test item

oxygen depletion : (lIlto - Il1tx) - (1lloo - IIlm), where:
mtO: oxygen concentration (mg/L) oftest item on day 0 (la and Ib, 2a and 2b or 4a and 4b from Table I)
mtx: oxygen concentration (mg/L) oftest item on day x (la and lb, 2a and 2b or 4a and 4b from Table I)
Ißt,o: oxygen concentration (mg/L) of inoculum blank on day 0 (mean of 3a and 3b)
mbx: oxygen concentration (mg/L) ofinoculum blank on day x (mean of3a and 3b)

 

Table 3. Percentage biodegradation, based on ThODNH4. at different time intervals during the exposure period of 28 days

Treatment	Conc. [mg/L]	flask No.	% biodegradation after n days exposure
			1	5	7	12	14	22	28
Test Item	2.12	1a	2	-1	1	1	1	-3	0
		1b	0	-1	-1	1	-1	-1	0
		mean	1	0	0	1	0	-2	0
Procedure Control (Aniline)	2.02	2a	0	30	56	65	73	83	99
		2b	0	22	56	65	71	79	94
		mean	0	26	56	65	72	81	97
Toxicity Control	TI: 2.04 Aniline: 2.14	4a	n.d	n.d	15	n.d	33	35	42
		4b	n.d	n.d	13	n.d	35	36	44
		mean	n.d	n.d	14	n.d	34	35	43

n.d.: not determined
TI: test item

BOD =  mg 02/L of test item and/or Aniline - mg 02/L of inoculum control  / mg test item and/or Aniline/L in flask

% biodegradation =  BODx 100% / ThODNH4 of Aniline and/or test item

 

where: ThO~H4 oftest item = 2.55 mg 02/mg test item

          ThO~H4 of Aniline = 2.41 mg 02/mg Aniline 

Applicant's summary and conclusion

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

Under the test conditions the percentage biodegradation of N-Isopropylnortropin was determined to be 0 % after 28 days of incubation, based
on ThODNNH4 and based on ThODN03.
The test item can be considered to be not ready biodegradable.