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EC number: 200-842-0 | CAS number: 75-12-7
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2003-Jul-30 - Aug-27
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 A (Ready Biodegradability: DOC Die Away Test)
- GLP compliance:
- yes
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Activated sludge from a laboratory wastewater treatment plant
- Method of cultivation: on a laboratory shaker, approx. 120 rpm at 22 +- 2 °C, fed with municipal sewage
- Pretreatment: Pre-aeration for about 24 h
- Concentration of sludge: 30 mg/L dry substance - Duration of test (contact time):
- 28 d
- Initial conc.:
- 20 mg/L
- Based on:
- DOC
- Initial conc.:
- 75 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- DOC removal
- Details on study design:
- TEST CONDITIONS
- Test temperature: 22 +- 2 °C
- Other: Incubation on a laboratory shaker, approx. 120 rpm
TEST SYSTEM
- Culturing apparatus: 2 L Erlenmeyer flasks
CONTROL AND BLANK SYSTEM
- Inoculum blank: 2
- Abiotic sterile control: 1
- Toxicity control: 1
- reference substance: 1
- Other: Adsorption control: 1 - Reference substance:
- aniline
- Remarks:
- 20 mg/L related to DOC
- Key result
- Parameter:
- % degradation (DOC removal)
- Value:
- 4
- Sampling time:
- 1 d
- Key result
- Parameter:
- % degradation (DOC removal)
- Value:
- 100
- Sampling time:
- 7 d
- Key result
- Parameter:
- % degradation (DOC removal)
- Value:
- 98
- Sampling time:
- 14 d
- Key result
- Parameter:
- % degradation (DOC removal)
- Value:
- 98
- Sampling time:
- 21 d
- Key result
- Parameter:
- % degradation (DOC removal)
- Value:
- 99
- Sampling time:
- 28 d
- Details on results:
- - duration of the adaptation phase (lag-phase):
ca. 2 days
- duration of the degradation phase (log-phase):
ca. 2 days
- physico-chemical (abiotic) elimination assay:
< 10 % (DOC) at the end of the test
- adsorption control:
< 10 % (DOC) after 5 days
- inhibition assay:
98 % (DOC) after 28 days
- Results with reference substance:
- Reference substance: aniline - concentration: 20 mg/L related to DOC
- kinetic of reference substance:
1 day(s) -5 % DOC-elimination
3 day(s) 7 % DOC-elimination
5 day(s) 94 % DOC-elimination
14 day(s) 98 % DOC-elimination
21 day(s) 100 % DOC-elimination
28 day(s) 100 % DOC-elimination - Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- The substance is readily biodegradable (~100% in 28 days)
Reference
Inhibition control:
-------------------
- substances: aniline + test substance
- concentration: aniline: 20 mg/L related to DOC
test subst.: 20 mg/L related to DOC
- kinetic of inhibition control:
1 day(s) |
1 % DOC-elimination |
3 day(s) |
18 % DOC-elimination |
7 day(s) |
97 % DOC-elimination |
14 day(s) |
99 % DOC-elimination |
21 day(s) |
98 % DOC-elimination |
28 day(s) |
98 % DOC-elimination |
Assay to examine physico-chemical (abiotic) elimination:
--------------------------------------------------------
- concentration: 20 mg/L related to DOC
- w/o inoculum, w mercury chloride to avoid microbial biodegradation
- kinetic of physico-chemical elimination:
1 day(s) |
-2 % DOC-elimination |
7 day(s) |
-2 % DOC-elimination |
14 day(s) |
-1 % DOC-elimination |
21 day(s) |
-5 % DOC-elimination |
28 day(s) |
-6 % DOC-elimination |
Adsorption control:
-------------------
- concentration: 20 mg/L related to DOC
- w inoculum, w mercury chloride to avoid microbial biodegradation
- kinetic of adsorption control:
1 day(s) |
-1 % DOC-elimination |
5 day(s) |
-5 % DOC-elimination |
Description of key information
Readily biodegradable (according to OECD criteria)
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
- Type of water:
- freshwater
Additional information
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