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EC number: 482-480-5 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 5 February 2007 to 3 July 2007.
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- The sludge was washed twice with tap water by centrifugation and the supernatant liquid phase was decanted. A homogenized aliquot of the final sludge suspension was weighed, dried and a ratio of wet to dry weight was calculated. An aliquot of washed sludge was suspended in tap water to obtain a concentration equivalent to 3 g dry material per liter. During the holding period of three days prior to use, the sludge was fed daily with 50 mL synthetic wastewater per liter and was kept at room temperature under continuous aeration until use. Before use, the dry weight of the activated sludge was measured again in the inoculum used for the test. The pH of the activated sludge inoculum was adjusted from 9.0 to 7.0 with a diluted sulphuric acid solution:
16 g peptone
11 g meat extract
3 g urea
0.7 g NaCL
0.4 g CaCL2 . 2H2O
0.2 g MgSO4 . 7H2O
2.8 g K2HPO4
At the start of the test, synthetic wastewater and activated sludge inoculum were added. The inoculum had a sludge concentration of 3.3 g/L dry weight. The sludge was added in time intervals of 15 minutes, first to a control and secondly to the test solutions of the reference item, thirdly to the test suspension of the test item, and finally to the second control. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 24 h
- Details on test conditions:
- - TEST SYSTEM
Test vessel: closed. 2000 mL glass beakers.
Aeration: during the incubation period of 3 hours, the single test medium and controls were continuously aerated by intense stirring on magnetic stirrers to avoid possible foaming and/or stripping of the test item.
Photoperiod: All test vessels were shielded from the light during mixing.
Temperature: 20°C at the start and the end of the incubation period.
- OTHER TEST CONDITIONS
The pH of the activated sludge inoculum was adjusted from 9.0 to 7.0 with a diluted sulphuric acid solution.
- TEST CONCENTRATIONS
The final nominal concentration tested was 1000 mg/L. - Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
The test substance had no significant inhibitory effect (15%) on the respiration rate of activated sludge after the incubation period of three hours at the limit test concentration of 1000 mg/L. Based on the physical appearance of the test suspension, the saturation concentration was < 1000 mg/L and resulted in less than 15% inhibition after the three hours of contact time.
Thus the 3-hour NOEC (EC15) of yje test substance to activated sludge microorganisms was at least 1000 mg/L. This value might have been even higher, but concentrations above 1000 mg/L were not tested. The 3-hour EC20, EC50, and EC80 could not be calculated, but were clearly higher than 1000 mg/L.- Results with reference substance (positive control):
- Results with reference substance valid? Yes
- Validity criteria fulfilled:
- yes
- Conclusions:
- In an OECD 209 study the effect of the test substance on the respiration of activated sewage sludge microorganisms gave a 3-hour NOEC (EC15) of at least 1000 mg/L.
- Executive summary:
The inhibitory effect of the test substance on the respiration rate of aerobic
wastewater microorganisms of activated sludge was investigated in a 3-hour respiration inhibition test according to the EU Commission Directive 88/302/EEC, Part C.11, and the OECD Guideline for Testing of Chemicals, No. 209.
In accordance with the EU Commission Directive 92/69/EEC (C.1, C.2, and C.3), a limit test with a nominal test concentration of 1000 mg/L was performed.
In addition, two controls and three different concentrations of the reference item
3,5-dichlorophenol (5, 16, and 50 mg/L) were tested in parallel. The results of these treatments confirmed suitability of the activated sludge and the method used.The test item had no inhibitory effect on the respiration rate of activated sludge after the incubation period of three hours at the test item concentration of 1000 mg/L. The saturation concentration (water solubility limit of the test substance under the test conditions) was reached and no inhibitory effect was determined after three hours contact time.
Thus, the 3-hour NOEC (EC15) of the test item to activated sludge microorganisms was at least 1000 mg/L. This value might even be higher, but concentrations above 1000 mg/L were not tested. The 3-hour EC20, EC50, and EC80 could not be calculated, but were clearly higher than 1000 mg/L.
Reference
Description of key information
In an OECD 209 study the effect of the test substance on the respiration of activated sewage sludge microorganisms gave a 3-hour NOEC (EC15) of at least 1000 mg/L.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 1 000 mg/L
- EC10 or NOEC for microorganisms:
- 1 000 mg/L
Additional information
The 3-hour NOEC (EC15) of coconut oil reaction products with boric acid and diethanolamine to activated sludge microorganisms was at least 1000 mg/L. This value might have been even higher, but concentrations above 1000 mg/L were not tested. The 3-hour EC20, EC50, and EC80 could not be calculated, but were clearly higher than 1000 mg/L (RCC, 2007).
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