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Diss Factsheets
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EC number: 248-339-5 | CAS number: 27215-95-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Acute Toxicity: dermal
Administrative data
- Endpoint:
- acute toxicity: dermal
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1993-09-13 to 1993-11-11
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: This study is classified as reliable without restrictions because it closely followed OECD Guideline 402 and is GLP compliant.
- Justification for type of information:
- A discussion and report on the read across strategy is given as an attachment in Section 13.
Cross-reference
- Reason / purpose for cross-reference:
- read-across: supporting information
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 993
- Report date:
- 1993
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 402 (Acute Dermal Toxicity)
- GLP compliance:
- yes
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- Hexadecene
- EC Number:
- 248-131-4
- EC Name:
- Hexadecene
- Cas Number:
- 26952-14-7
- Molecular formula:
- C16H32
- IUPAC Name:
- hexadecene
- Details on test material:
- - Name of test material (as cited in study report): Sample 1205-48 (Isomerised C16 Olefin)
- Substance type: C16 isomerised olefin
- Physical state: Clear colourless liquid
Constituent 1
Test animals
- Species:
- rabbit
- Strain:
- New Zealand White
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Ray Nichols Rabbitry, Lumberton, Texas
- Age at study initiation: Young adult 3 to 6 months
- Weight at study initiation: Males (2.425 to 2.875 kilograms); Females (2.400 to 2.750 kilograms)
- Housing: Suspended, wire bottom, stainless steel; one animal per cage
- Diet (e.g. ad libitum): Purina Rabbit Chow; fed in measured amounts
- Water (e.g. ad libitum): Tap water; ad libitum
- Acclimation period: 5 days
Administration / exposure
- Type of coverage:
- occlusive
- Vehicle:
- unchanged (no vehicle)
- Details on dermal exposure:
- TEST SITE
- Area of exposure: Dorsal surface of the trunk
- % coverage: 10%
- Type of wrap if used: Surgical gauze covered with non-irritating adhesive tape which was further covered with semi-permeable dressing and again wrapped with non-irritating adhesive tape
REMOVAL OF TEST SUBSTANCE
- Washing (if done): Washed with room temperature tap water and a clean wet cloth
- Time after start of exposure: 24 hours
TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 2.37 mL/kg equivalent to 2020 mg/kg
- Concentration (if solution): Not applicable
- Constant volume or concentration used: Yes
- Duration of exposure:
- 24 hours
- Doses:
- 2020 mg/kg
- No. of animals per sex per dose:
- 5 males and 5 females
- Control animals:
- not specified
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: 0.5, 3, and 6 hours after exposure and daily until day 14
- Necropsy of survivors performed: Yes
- Other examinations performed: Clinical signs and body weight - Statistics:
- Statistical analysis, if performed, is not presented in the study report.
Results and discussion
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 2 020 other: mg/kg
- Mortality:
- One male died on day 14 of the study after all study observations were made. The authors reported that this death is not related to treatment.
- Clinical signs:
- other: No notable observations were reported.
- Gross pathology:
- The male rabbit that died on day 14 of the study had mottled lungs with white nodules throughout. However, this observation was considered to be unrelated to treatment. None of the other animals exhibited any abnormalities.
Any other information on results incl. tables
Body weight gain was not affected from exposure to hexadecene. However, one male that died on day 14 of the study lost weight during the study. Toxicological and pharmacological signs were not noted. There were no signs of dermal irritation during the 14 day observation period. Gross necropsy at study termination revealed no observable abnormalities in most animals. However, one male that died on day 14 of the study revealed mottled lungs with white nodules everywhere. None of the other treated animals died during the course of the study. Based on these results, the LD50for hexadecene in albino rabbits is > 2020 mg/kg (2.37 mL/kg).
Applicant's summary and conclusion
- Interpretation of results:
- other: Not classified
- Remarks:
- Not classified because LD50 is greater than the requirements for a Category 4 toxicant (2000 mg/kg) Criteria used for interpretation of results: EU
- Conclusions:
- The acute dermal LD50 for hexadecene is greater than 2020 mg/kg.
- Executive summary:
In acute dermal toxicity study, five male and female New Zealand White rabbits were clipped free of hair on the dorsal surface of the trunk and treated with 2020 mg/kg (2.37 mL/kg) undiluted hexadecene that was held in contact with the skin for 24 hours. After 24 hours the animals were observed for toxicological and pharmacological effects and mortality at 0.5, 3, and 6 hours after treatment and at least once daily until study termination on day 14. Animals were sacrificed for necropsy on day 14.
Body weight gain was not affected from exposure to hexadecene. However, one male that died on day 14 of the study lost weight during the study. Toxicological and pharmacological signs were not noted. There were no signs of dermal irritation during the 14 day observation period. Gross necropsy at study termination revealed no observable abnormalities in most animals. However, one male that died on day 14 of the study revealed mottled lungs with white nodules everywhere. None of the other treated animals died during the course of the study. Based on these results, the LD50for hexadecene in albino rabbits is > 2020 mg/kg (2.37 mL/kg).
This study received a Klimisch score of 1 and is classified as “reliable without restrictions” because it closely followed OECD Guideline 402 and is GLP compliant. This study will influence the DNEL(s).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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