Basic copper zinc nitrate

Toxicity to aquatic plants other than algae

Administrative data

Endpoint:

toxicity to aquatic plants other than algae

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Justification for type of information:

Due to the similar chemical structure or properties, a read-across is selected for the determination for the toxicity to aquatic plants other than algae

Cross-reference

Reason / purpose for cross-reference:

read-across source

Reference

Endpoint:

toxicity to aquatic plants other than algae

Type of information:

experimental study

Adequacy of study:

weight of evidence

Study period:

n.a.

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: no oecd guidline, no glp information

Qualifier:

no guideline followed

Principles of method if other than guideline:

Nasturtium officinale (watercress) was
collected from a natural pond in Burdur, Turkey.
The plants were kept at a temperature
of 25±2 °C and illuminated by cool daylight
fluorescent tubes in 14 h light and 10 h dark cycle.
In all the experiments 4.8 g. plant samples (fresh
weight) were placed in 100 m/l of solution and kept
under natural conditions. Before the start of the
experiment the plants were cleaned properly using
tap water to remove particles from their roots and
leaves The aquatic plant was exposed separately to
the individual metal ion solutions of copper (CuSO4.
5H2O) zinc (ZnSO4. H2O) and nickel (NiCl2. 6H2O)
at nominal concentrations of 1.0, 3.0, 5.0 and 7.0
mg/l. The test plants exposed to heavy metals were
harvested 24, 48 and 72 h after treatment and
weighed. The harvested plant samples were dried
at 80 °C. Both plants and water samples were
digested in 99% HNO3: HClO4 (5:1) (AR, E. Merck,
Germany). One control group was also used without
the addition of any metal. The aquatic plants grown
in the solutions were weighed after 4 days and
analyzed for metal ion concentration in both plants.
Copper, zinc and nickel were measured by an Atomic
Absorption Spectrophotometer Shimadzu Model AA
640-F.

GLP compliance:

not specified

Analytical monitoring:

not specified

Vehicle:

no

Test organisms (species):

other: Nasturtium officinale

Test type:

not specified

Water media type:

saltwater

Limit test:

no

Total exposure duration:

15 d

Remarks on result:

other: see conclusion

Validity criteria fulfilled:

not specified

Conclusions:

Copper accumulates in water plants in water with a high copper concentration, after changing to "normal" water removal of copper.

Materials and methods

Test material

Test material information

Constituent 1

Reference substance name:

copper zinc trihydroxide nitrate

Molecular formula:

CuZn(OH)3NO3

IUPAC Name:

copper zinc trihydroxide nitrate

Test material form:

solid

Results and discussion

Effect concentrations

Remarks on result:

other: see conclusion

Applicant's summary and conclusion

Conclusions:

Copper accumulates in water plants in water with a high copper concentration, after changing to "normal" water removal of copper.