[No public or meaningful name is available]

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

June 7-9, 1989

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1989

Report date:

1989

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

no

Remarks:

Study carried out before GLP was required.

Test material

Test material information

Constituent 1

Reference substance name:

7-hydroxycitronellal

EC Number:

203-518-7

EC Name:

7-hydroxycitronellal

Cas Number:

107-75-5

Molecular formula:

C10H20O2

IUPAC Name:

7-hydroxy-3,7-dimethyloctanal

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test medium of the highest test concentration of nominal 500 mg/l was prepared.
Subsequently the stock solution was diluted serially with test water to obtain the desired test c
oncentrations.
- Controls: Yes, negative control
- Test concentration separation factor: 2-fold

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Strain/clone: Straus
- Age at study initiation (mean and range, SD): 2-24 hrs
- Source: originally obtained from the Institute National de Recherche Chimique Appliquee, France
and cultured in the laboratories of BASF since 1978.

ACCLIMATION
- Food type: Green algae culture
- Frequency: daily

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

2.66 mmol/l

Test temperature:

293.2 K

pH:

6.31-9.25 mg/L

Dissolved oxygen:

8.61-9.25 mg/l

Salinity:

Na:K 10:1
Ca:Mg 4:1

Conductivity:

640 uSiemans/cm

Nominal and measured concentrations:

0, 31.25, 62.5, 125, 250, 500 mg/L (nominal)

Details on test conditions:

TEST SYSTEM
- Test vessel: Centrifuge glasses with flat bottoms
- Material, size, headspace, fill volume: 10 mL
- Aeration: Aerated with oil-free air for 24 hrs
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 2 mL/animal

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: deionized water
- Metals: 0.45 mg/L
- Chlorine: 144.9 mg/L
- Ca/mg ratio: 4:1
- Conductivity: 600-700 uSiemens/cm

OTHER TEST CONDITIONS
- Photoperiod: 16 hrs light/8 hrs dark
- Light intensity: 5 microEinsteins (m2s) in the wavelength range 400-750 nm

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Mobility at 0, 3, 6, 24, and 48 hrs

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

> 500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

250 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

410 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- Immobilisation of control: None

Any other information on results incl. tables

Number Mobile

 

Concentration (mg/L)	0 hrs	3 hrs	6 hrs	24 hrs	48 hrs
0	20	20	20	20	20
31.25	20	20	20	20	20
62.5	20	20	20	20	20
125	20	20	20	20	20
250	20	20	20	20	18
500	20	20	20	15	5

 

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The 48-hr EC50 of the test substance was 410 mg/L.

Executive summary:

The toxicity of the test substance to daphnids was determined.  Daphnids were exposed to concentrations of 0, 31.25, 62.5, 125, 250, and 500 mg/L (nominal) of test substance.  20 daphnids per concentration were exposed.  They were then observed for 48 hrs for mobility.  The 48-hr EC50 was determined to be 410 mg/L (nominal).