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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Water solubility
Administrative data
- Endpoint:
- water solubility
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study without detailed documentation
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 020
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 105 (Water Solubility)
- Deviations:
- not specified
- Principles of method if other than guideline:
- Various amounts of CDX-403 are mixed with 5 mL of water. This was rotated at 30°C overnight to dissolve. This solution was centrifuges at 4000 rpm for 30 minutes and visually inspected for insoluble lyophilized enzyme powder. The highest concentration with no visual insoluable matter is reported as the solubility of CDX-403.
- GLP compliance:
- not specified
- Type of method:
- flask method
Test material
- Reference substance name:
- Halohydrin dehalogenase
- Cas Number:
- 132421-41-1
- IUPAC Name:
- Halohydrin dehalogenase
- Test material form:
- solid
- Details on test material:
- Lot number PF 791
Constituent 1
- Specific details on test material used for the study:
- (lot# PF 791)
Results and discussion
Water solubility
- Key result
- Water solubility:
- 655 other: mg/mL
- Conc. based on:
- test mat.
- Incubation duration:
- 24 h
- Temp.:
- 30 °C
- Remarks on result:
- other: there is no data availble on the pH
- Details on results:
- The highest concentration of CDX-403 in water is achieved at 655 mg/mL. At this concentration, the solution is in a gel-like state. Adding additional lyophilized powder into this solution will making mixing impossible. As a result, the CDX-403 solubility is reported as 655 mg/mL in water.
Any other information on results incl. tables
The final concentration of CDX-403 is calculated based on the amount of lyophilized powder added and the final volume of the solution (Table 1). The UV280nm absorbance of each solution is measured to confirm that the OD is within linear range (Figure 1, attached in illustrations).
Table 1. Final volume and amounds (mg) of CDX-403 in water (mL)
Weight of CDX-403 (mg) | Water (mL) | Total Volume (mL) |
51.9 | 5.00 | 5.05 |
119.1 | 5.00 | 4.58 |
306.2 | 5.00 | 5.25 |
594.4 | 5.00 | 5.50 |
1002 | 5.00 | 5.80 |
2014 | 5.00 | 6.50 |
4000 | 5.00 | 8.00 |
6000 | 5.00 | 9.16 |
6000 | 5.00 | 9.61 |
Applicant's summary and conclusion
- Conclusions:
- The water solubility is 655 mg/mL in water.
- Executive summary:
For solubility in water, various amounts of CDX-403 were mixed with 5 mL of water. Rotate at 30°C overnight to dissolve. Centrifuge 4000 rpm for 30 minutes and visually observe insoluble lyophilized enzyme powder. The highest concentration is reported as the solubility of CDX-403.
The highest concentration of CDX-403 in water was achieved at 655 mg/mL. At this concentration, the solution is in a gel-like state. Adding additional lyophilized powder into this solution will making mixing impossible. As a result, the CDX-403 solubility is reported as 655 mg/mL in water.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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