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EC number: 684-879-1 | CAS number: 848641-69-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 18-4-2019 to 03-09-2019
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 019
- Report date:
- 2019
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- other: Chemicals - ready biodegradability - Manometric respirometry test
- Version / remarks:
- (GB/T 21801-2008)
- Qualifier:
- according to guideline
- Guideline:
- other: The Guidelines for the testing of Chemicals, Degradation and Accumulation
- Version / remarks:
- 2nd edition, Bejing, China Environmental Press, 2013
- Qualifier:
- according to guideline
- Guideline:
- other: The guidelines for the testing of chemicals
- Version / remarks:
- SEPA (HJ/T 153-2004)
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Version / remarks:
- July 1992
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- 1-Ethyl-3-methylimidazolium Diethyl Phosphate
- Cas Number:
- 848641-69-0
- Molecular formula:
- C6H11N2 C4H10O4P
- IUPAC Name:
- 1-Ethyl-3-methylimidazolium Diethyl Phosphate
- Test material form:
- liquid
- Details on test material:
- Batch No 187-9-113-Y
Expiry date: 2021-1-30
Constituent 1
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch number of test material: 187-9-113-Y
- Expiration date of the lot/batch: 2021-01-30
- Purity test date: 2019-03-06
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: closed container, dry and cool place, well ventilated
Test substance stock solution was prepared by mixing 1.0225 g of test substance with mineral medium and fill up to 100 ml.
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Details on inoculum:
- - Source of inoculum/activated sludge: Sludge originated from a sewage treatment plant with predominantly domestic sewage (Nanjing Tiebei Sewage treatment plant, Batch No.: AS20190423)
- Preparation of inoculum for exposure: Sludge was washed with basal mineral medium by settling the sludge in a graduate cylinder, decanting the supernatant liquid, replacing with fresh basal mineral medium, aerating and settling. The above process was repeated 3 times and the sludge was aerated until use.
- Pretreatment: Before use, the sludge was centrifuged (5000 r/min, 10 minutes) and the supernatant liquid decanted. Thereafter, a small amount of the washed sludge was weighed and then dried at 100°C until the weight of the dried sludge did not change significantly anymore. Then the dry weight percentage of suspended solids of the activated sludge was calculated (5.63%). Finally 3.55 g wet sludge after centrifugation was mixed with 50 ml basal mineral medium to obtain an activated sludge with a mixed liquid suspended solids level of 4 g SS/L. The microbial inoculum concentration was 30 mg/L.
- Initial cell/biomass concentration: Cell density was determined to be 4.4x10-7 CFU/L using microscope. - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 44.9 mg/L
- Based on:
- ThOD
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium:
Stock solution A:
8.56 g KH2PO4
21.7 g K2HPO4
33.39 g NA2HPO4*2H2O
0.51 g NH4CL
in 1 litre deionized water.
Stock solution B: 27.45 g CaCl2 in 1 l deionized water.
Stock solution C: 22.5 g MgSO4*7H2O in 1 l deionized water.
Stock solution D: 0.25 g FeCl3*6H2O in 1 l deionized water.
Basal stock medium: 10 ml Stock solution A in 800 ml deionized water, addition of 1 ml each of solution B, C and D, fill up to 1 litre.
- Additional substrate:
- Test temperature: 22°C +/- 1°C
- pH: 7.2 to 7.67, measured at test start and end in each bottle
- pH adjusted: no
- Suspended solids concentration: 30 mg/L
- Continuous darkness: yes
- Other:
TEST SYSTEM
- Culturing apparatus: closed glas vessel (510 ml) with OxiTop BOD meter, final test solution volume: 250 ml
- Number of culture flasks/concentration: 2
- Details of trap for CO2: absorption by NaOH
- Other: All vessels contained a Teflon coated magnetic stir bar. After equipment assembly stirrer was started and the equipment was checked for air-tightness. Vessels were kept at 22°C in the dark.
CONTROL AND BLANK SYSTEM
- Inoculum blank: 2 vessels containing inoculum only
- Procedure control: 1 vessel containing reference substance (40 mg/L) and inoculum
- Toxicity control: 1 vessel containing test substance (44.9 mg/l), reference substance (40 mg/L) and inoculum
- Other:
STATISTICAL METHODS:
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
- Test performance:
- Biodegradation of the toxicity control was 50.3% in 14 days indicationg that there is no inhibitory effect ot the test item.
% Degradation
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 35
- Sampling time:
- 28 d
BOD5 / COD results
- Results with reference substance:
- Biodegradation of the reference substance, sodium benzoate, reached 97% within 14 days.
Any other information on results incl. tables
see attachments
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Executive summary:
The ready biodegradability of the test item was determined in a 28-d oxygen consumption test (OECD 301F) using activated sludge from a domestic waste water treatment plant. The initial concentration of the test item was 44.9 mg/l, the inoculum concentration was 30 mg/L.
Biodegradation of the reference substance, sodium benzoate, reached 97% within 14 days. Biodegradation of the toxicity control was 50.3% in 14 days indicating that there is no inhibitory effect ot the test item.
Under the test conditions the test substance was degraded by 35% after 28 days.
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