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EC number: 220-284-1 | CAS number: 2700-30-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From February 02, 2021 to February 08, 2021
- Reliability:
- 1 (reliable without restriction)
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 13th April 2004
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Batch number: M-O303190723301F
Purity: 99.68% - Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 100 mg/L
- Sampling method: Samples were taken for analysis from the freshly prepared solutions at 0 hour and the old solutions at 48 hours.
- Sample storage conditions before analysis: - Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 0.0501 g test item was weighed and dissolved in 500 mL of test water directly. After the test item was dissolved completely, the solution of 100 mg/L was used for exposure.
- Controls: dilution water (without test item)
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): water - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Strain/clone:
- Justification for species other than prescribed by test guideline:
- Age at study initiation (mean and range, SD): less than 24 hours old and not the first brood progeny.
- Source: Daphnia of stock culture was originally obtained from Applied Chemistry Department Pesticide Environmental Evaluation Laboratory of China Agricultural University.
ACCLIMATION
- Acclimation period: 24 hours
- Acclimation conditions (same as test or not): same
- Health during acclimation (any mortality observed): showing no signs of stress such as high mortality, presence of males and ephippia, delay in the production of the first brood, discolored animals - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Hardness:
- 140 to 250 mg/L (as CaCO3)
- Test temperature:
- 18~22oC
- pH:
- 6.0 to 8.5 pH units
- Dissolved oxygen:
- not less than 3 mg/L during the test period
- Nominal and measured concentrations:
- 100 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 200 mL glass beakers
- Type (delete if not applicable): open / closed
- Material, size, headspace, fill volume:
- Volume of solution: 100 mL per vessel
- Aeration: Before usage, the water was aerated for more than 24 hours to attain dissolved oxygen saturation.
- Type of flow-through (e.g. peristaltic or proportional diluter):
- Renewal rate of test solution (frequency/flow rate): no
- No. of organisms per vessel: 5 daphnia
- No. of vessels per concentration (replicates): 4 replicates
- No. of vessels per control (replicates): 4 replicates
- No. of vessels per vehicle control (replicates):
- Biomass loading rate:
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: the dilution water was tap water after being treated with Aquapro Pure Water System
- Total organic carbon: < 2 mg/L
- Intervals of water quality measurement: in all fresh and old media for both control and test group.
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 hr light: 8 hr dark
- Light intensity:
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Observation intervals: 24(±1) and 48(±1)hours after exposure
Method: Each test vessel was checked for immobilized daphnids. In addition to immobility, any abnormal behavior or appearance were recorded. Those animals that are not able to swim within 15 seconds, after gentle agitation of the test vessel are considered to be immobilized (even if they can still move their antennae).
VEHICLE CONTROL PERFORMED: yes
RANGE-FINDING STUDY
- Test concentrations: 1, 10, 100 mg/L
- Results used to determine the conditions for the definitive study: During the period of 48 hours, no immobilization or any toxic signs were observed in the control group and the test groups of 1, 10 and 100 mg/L. - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate as reference item was tested for EC50 as a means of assuring that the test conditions are reliable four times a year in the laboratory.
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- During the 48-hour exposure period, no immobilization and abnormal behavior or appearance was observed in the control group and the test group of 100 mg/L.
- Results with reference substance (positive control):
- EC50-24 h of potassium dichromate to Daphnia magna was 1.17 mg/L, and the 95% confidence limits was 0.99 ~ 1.39 mg/L. The results met the recommended criterion in OECD Guideline No. 202: EC50-24 h of potassium dichromate is within the range of 0.6 mg/L to 2.1 mg/L.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 48-hour EC50 of the test item to Daphnia magna was greater than 100 mg/L.
- Executive summary:
A Daphnia acute immobilisation test of the test item was conducted with Daphnia magna over a 48-hour exposure period, according to OECD Guideline 202 under GLP.
According to the results of test and based on the nominal concentration, the 48-hour EC50 of the test item to Daphnia magna was greater than 100 mg/L.
Reference
Description of key information
A Daphnia acute immobilisation test of the test item was conducted with Daphnia magna over a 48-hour exposure period, according to OECD Guideline 202 under GLP.
According to the results of test and based on the nominal concentration, the 48-hour EC50 of the test item to Daphnia magna was greater than 100 mg/L.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Dose descriptor:
- EC50
- Effect concentration:
- > 100 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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