Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 416-530-4 | CAS number: 178949-82-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From June 29, to July 09, 1993
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 0, 100, 320 and 1000 mg/L
- Sampling method: samples were taken at t = 0 h (newly prepared solutions) and t = 24 h (spent solutions after 24 h). Samples of ca. 100 mL were taken in polyethylene bottles and preserved by the addition of formol (1% final active concentration).
- Sample storage conditions before analysis: samples were stored in a refrigerator. - Vehicle:
- no
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Zebrafish
- Source: commercial hatchery Romberg's Aquariumhuis (Hugo de Grootplein 25, Delft)
- Weight at study initiation (mean and range, SD): 0.12 ± 0.02 g
- Length at study initiation (mean and range, SD): 2.5 ± 0.2 cm
- Feeding during test: no
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Post exposure observation period:
- None
- Hardness:
- 224 mg/L (expressed as CaCO3)
- Test temperature:
- 24.7-25.5oC (see Table 1 for details)
- pH:
- 7.0-7.9 (see Table 2 for details)
- Dissolved oxygen:
- The lowest value measured was 7.0 mg/L (see Table 3 for details)
- Salinity:
- No data
- Nominal and measured concentrations:
- 100, 180, 320, 560 and 1000 mg/L (nominal)
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: two L all-glass beakers, each containing 1.5 L of test solution or control medium.
- Aeration: Slightly aerated
- Renewal rate of test solution: replaced daily
- No. of organisms per vessel: ten
- No. of vessels per concentration: one
- No. of vessels per control: one
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: the dilution water used was DSWL, and prepared by addition of several salts to groundwater from a locality near Linschoten (The Netherlands).
- Total organic carbon: 1.9 mg/L
- Composition: The following components were measured by chemical analysis and found to be:
Na+ 1.33 mmol/L
K+ 0.20 mmol/L
Ca+2 1.42 mmol/L
Mg+2 0.82 mmol/L
Cl- 2.81 mmol/L
SO4-2 0.55 mmol/L
OTHER TEST CONDITIONS
- Photoperiod: 16 h light-8 h dark with transition periods of ca. 30 minutes
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality, condition (swimming behaviour, colour, respiratory function or any other visually observable morphological or behavioural criterion) of the survivors were compared with that of the control animals.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.8
- Range finding study
- Test concentrations: no data
- Results used to determine the conditions for the definitive study: no data - Reference substance (positive control):
- no
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: No deaths seen at highest concentration
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- ca. 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- ca. 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- behaviour
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- ca. 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- morphology
- Details on results:
- - Behavioural abnormalities: no data
- Other biological observations: swimming behaviour, colour, respiratory function or any other visually observable morphological or behavioural criterion were comparable to that of the control animals.
- Mortality of control: none
- Other adverse effects control: no data
- Abnormal responses: no data - Results with reference substance (positive control):
- Not applicable
- Reported statistics and error estimates:
- Not applicable
- Sublethal observations / clinical signs:
Table 4: Number of fish and their condition in the control medium and in the test substance solutions during the test
Time (h)
Nominal concentration of test substance (mg/L)
0
100
180
320
560
1000
0
10a)
10a)
10a)
10a)
10a)
10a)
24
10a)
10b)
10b)
10b)
10b)
10b)
48
10a)
10b)
10b)
10b)
10b)
10b)
72
10a)
10b)
10b)
10b)
10b)
10b)
96
10a)
10b)
10b)
10b)
10b)
10b)
where,
a) Condition of all fish, visually assessed, normal (= good)
b) Condition of all fish, visually assessed, equal to that of the control fish
- Validity criteria fulfilled:
- yes
- Conclusions:
- In a GLP study conducted according to OECD Guideline 203, trisodium EDDS was not acutely toxic to the freshwater fish, Brachydanio rerio, when tested up to a nominal concentration of 1000 mg/L, therefore the 96-h NOEC value for survival and condition was 1000 mg/L.
- Executive summary:
A GLP study conducted according to OECD Guideline 203 was performed to determine the acute toxicity of trisodium EDDS to Brachydanio rerio (10 fish/concentration). The test was carried out under semi-static conditions with daily replacement of the test solutions. Fish were exposed to trisodium EDDS in freshwater at nominal concentrations of 0, 100, 180, 320, 560 and 1000 mg/L and observed for survival, condition (swimming behaviour, colour, respiratory function or any other visually observable morphological or behavioural criterion) of the survivors after 24, 48, 72 and 96 h of exposure.
After 96 h, all Zebrafish were alive and their condition was comparable to that of the control animals. All test solutions were completely clear (visually assessed) throughout the test.
In conclusion, the 96-h NOEC for survival and condition of this freshwater fish was 1000 mg/L (the highest tested nominal concentration), and the 96-h LC50 would therefore be >1000 mg/L.
Reference
Description of key information
In a GLP study conducted according to OECD Guideline 203, trisodium EDDS was not acutely toxic to the freshwater fish, Brachydanio rerio, when tested up to a nominal concentration of 1000 mg/L, therefore the 96-h NOEC value for survival and condition was 1000 mg/L.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Dose descriptor:
- LC50
- Effect concentration:
- > 1 000 mg/L
Additional information
A GLP study conducted according to OECD Guideline 203 was performed to determine the acute toxicity of trisodium EDDS to Brachydanio rerio (10 fish/concentration). The test was carried out under semi-static conditions with daily replacement of the test solutions. Fish were exposed to trisodium EDDS in freshwater at nominal concentrations of 0, 100, 180, 320, 560 and 1000 mg/L and observed for survival, condition (swimming behaviour, colour, respiratory function or any other visually observable morphological or behavioural criterion) of the survivors after 24, 48, 72 and 96 h of exposure. After 96 h, all animals were alive and their condition was comparable to that of the control animals. All test solutions were completely clear (visually assessed) throughout the test. In conclusion, the 96-h NOEC for survival and condition of freshwater fish was 1000 mg/L (the highest tested nominal concentration), and the 96-h LC50 would be >1000 mg/L (Hooftman and van Drongelen-Sevenhuijsen, 1993).
No data in marine fish currently available.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.