1,1,2,3,3,6-hexamethylindan-5-yl methyl ketone

Acute Toxicity: other routes

Administrative data

Endpoint:

acute toxicity: other routes

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1977

Report date:

1977

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

Three groups of 5 female SD rats were exposed to 100, 215, and 464 mg/kg bw via i.p. and mortality was determined for 7 days.

GLP compliance:

no

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

1,1,2,3,3,6-hexamethylindan-5-yl methyl ketone

EC Number:

239-360-0

EC Name:

1,1,2,3,3,6-hexamethylindan-5-yl methyl ketone

Cas Number:

15323-35-0

Molecular formula:

C17H24O

IUPAC Name:

1,1,2,3,3,6-hexamethylindan-5-yl methyl ketone

Test material form:

solid

Specific details on test material used for the study:

- Description: Clear liquid (super saturated)

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Age at study initiation: 121 - 172 g
- Fasting period before study: overnight

Administration / exposure

Route of administration:

intraperitoneal

Vehicle:

ethanol

Details on exposure:

100 and 215 mg/kg bw: 25% in ethanol
464 mg/kg bw: undiluted

Doses:

100, 215, 464 mg/kg bw

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

- Duration of observation period following administration: 7 days
- Frequency of observations: Observations for mortality and signs of effects were made immediately after dosing at one, four, and 24 hours, and once daily thereafter
- Necropsy of survivors performed: yes

Statistics:

Mortality data was analyzed statistically, utilizing the tables of Horn H.J., Biometrics 12, 311, 1956

Results and discussion

Effect levels

Sex:

female

Dose descriptor:

LD50

Effect level:

271 mg/kg bw

Based on:

test mat.

95% CL:

>= 167 - <= 441

Mortality:

100 mg/kg bw: No mortality was observed
215 mg/kg bw: Two animals died within 1 day
464 mg/kg bw: Two animals died within 1 day. Two other animals died one day later.

Clinical signs:

100 mg/kg bw: None
215 mg/kg bw: One of the animals who died exhibited slightly depressed behavior. This animal was also stained on the body fur with urine, which indicates a lack of normal preening.
464 mg/kg bw: One animal who died was prostrate.

Gross pathology:

100 mg/kg bw: None
215 mg/kg bw: The two animals who were found dead exhibited expected effects of their stages of autolysis
464 mg/kg bw: Two animals exhibited yellow coloration in the large intestines and another exhibited a lighter coloration of the brain than would normally be expected. All other animals in the study exhibited no remarkable effects.