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Diss Factsheets
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EC number: 232-615-7 | CAS number: 9001-57-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Data based on the inhibition control of a ready biodegradability study. This approach is in accordance with the Guidance on information requirements and chemical safety assessment (Chapter R.7b: Endpoint specific guidance, ECHA 2017).
- Reason / purpose for cross-reference:
- reference to same study
- Qualifier:
- according to guideline
- Guideline:
- other: OECD 301E
- GLP compliance:
- yes (incl. QA statement)
- Test organisms (species):
- activated sludge
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Domestic waste water treatment plant : ARA Ergolz II, Füllinsdorf, Switzerland
- Preparation of inoculum for exposure: The sludge was washed three times with tap water and an amount corresponding to 4g/L dry material (± 10%) was mixed with Sörensen buffer solution pH7 and then aerated prior to incubation. An amount of 0.5 mL sludge (filtered over cotton wool) was added to 1000 mL test medium. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 28 d
- Details on test conditions:
- TEST CONDITIONS
- Composition of medium: according to OECD Guideline No. 301 E
- Test temperature: 19.7 - 21.5°C
- Continuous darkness: yes
SAMPLING
- Sampling frequency: day 0 (treatment day) , 7, 14, 21, 27 and 28
- Sampling method: Per sampling interval, two flasks of the samples containing the test article or the reference compound, one flask of the inoculum blank and one flask of the toxicity control were taken and analysed for DOC in triplicate. - Reference substance (positive control):
- yes
- Remarks:
- aniline
- Duration:
- 28 d
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 45 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Result of toxicity control from ready biodegradability test
- Duration:
- 28 d
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 25.65 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Remarks:
- active enzyme protein
- Basis for effect:
- other: Result of toxicity control from ready biodegradability test
- Results with reference substance (positive control):
- The reference compound aniline reached complete biodegradation during the first seven exposure days. At the end of the test, aniline was readily biodegraded by an average of 95.1%.
Reference
Description of key information
NOEC (28 d):≥25.65 mg aep/L (OECD 301F; read-across)
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 25.65 mg/L
Additional information
Studies investigating the toxicity of β-fructofuranosidase to microorganisms are not available. An inhibition of the activity of activated sludge organisms is not expected based on the readily biodegradability of the substance. The enzyme β-fructofuranosidase is expected to be readily biodegradable based on read-across data, available for the sourceα-amylase. In the available test, conducted according to OECD guideline 301F, a degradation rate of 99% after 28 days at a test substance concentration of 44 mg/L was determined. Based on the results of the ready biodegradability study, no inhibition of the activity of activated sludge microorganisms by the source substance is expected. It is assumed that these conclusions can also be applied to the target substance. Hence, based on the available information, β-fructofuranosidase is not expected to be toxic to aquatic microorganisms.
Since no studies on the toxicity to aquatic microorganisms are available, the ready biodegradability study is used to derive a NOEC for the toxicity to aquatic microorganisms. If a compound degrades well in a ready biodegradability test, or does not inhibit the degradation of a positive control at a certain concentration, this concentration can be used as a NOEC value. This approach is in accordance with the Guidance on information requirements and chemical safety assessment (Chapter R.7b: Endpoint specific guidance, ECHA 2017).
The available ready biodegradability study according to OECD 301F (read-across) includes a toxicity control, which contains 25.65 mg/L active enzyme protein and 26 mg/L of the reference material aniline. The reference compound aniline reached complete biodegradation during the first seven exposure days. At the end of the test, aniline was readily biodegraded by an average of 95.1%. The test substance was degraded by 99% after 28 days. Therefore, the substance is expected to be not toxic to aquatic microorganisms in the toxicity control and the test item concentration of 25.65 mg/L can be used as NOEC.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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