N-{[Benzyl(ethyl)amino]-[(chloro-cyano-nitrophenyl)substituted]phenyl}acetamide

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

The prepared test concentration along with the negative control was analyzed for the test item concentration at the beginning and end of test. For analysis, single composite sample was drawn from prepared test concentration and duplicate sample was drawn from the negative control.

Vehicle:

yes

Remarks:

reconstituted water

Details on test solutions:

To prepare test concentration of 100 mg/L, 100 mg test item was mixed and made up to 1L in a volumetric flask using test medium separately.
For negative control (GI), 1L test medium was used.

A stock solution of reference substance (Potassium dichromate) at 1 mg/mL was prepared by mixing 10 mg in 10 mL of test medium. For preparation of reference substance at 0.32 mg/L (G3), 0.32 mL of reference substance stock was mixed and made up to 1L in a volumetric flask using test medium.

150 mL of prepared negative control, test item concentrations and reference substance was dispensed separately into 4 test containers.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Age at study initiation (mean and range, SD): Less than 24 hours (Neonates)
- Source: Ecotoxicology lab, Eurofins Advinus ltd.
- Feeding during test: Chlorella Vulgaris.
- Food type: unicellular green alga.

ACCLIMATION
- Acclimation conditions (same as test or not): Yes
- Type and amount of food: Chlorella Vulgaris
- Health during acclimation (any mortality observed): No

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test temperature:

Range finding : 20.3 to 20.5 degree celcius
Definitive test : 20.3 to 20.4 degree celcius

pH:

Range finding : 7.70 to 7.81
Definitive test : 7.79 to 7.81

Dissolved oxygen:

Range finding : 7.0 to 7.4
Definitive test : 7.0 to 7.3

Nominal and measured concentrations:

Range finding test: 0.00001, 0.0001, 0.001, 0.01, 0.1, 1, 10 and 100 mg/L
Limit test: 100 mg/L

Details on test conditions:

VEHICLE CONTROL PERFORMED: yes

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Key result

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

Range finding study : No immobility of daphnia was observed in negative control and at any of the tested concentration.
Main study: No immobility observed with negative control and at 100 mg/L test concentration.

Results with reference substance (positive control):

At 0.32 mg/L reference substance, the immobilization of daphnia was 50 and 60% at 24 and 48 hours exposure respectively.

Validity criteria fulfilled:

yes

Conclusions:

The EC50 value for FAT 41047/A TE at 48 hours is higher than 100 mg/L.

Executive summary:

The acute immobilization effect of the test item FAT 41047/A TE was studied on Daphnia magna according to OECD test guideline 202.

 

A range finding test was carried out to determine the test concentration for the definitive test, where Daphnia magna less than 24 hours old were exposed to the test item concentrations of 0.00001, 0.001, 0.01, 0.1, 1.0, 10 and 100 mg/L along with the negative control in test medium (reconstituted water) at 20 ± 1 °C. No immolisation was observed at any of the test concentrations during the range finding test.

Based on the results of range finding test, in definitive test, Daphnia magna less than 24 hours old were exposed to limit test concentrations of 100 mg/L along with a negative control and a reference substance, potassium dichromate at 0.32 mg/L in test medium (reconstituted water) at 20 ± 1 °C. The number of daphnia immobilized was recorded at 24 and 48 hours exposure.

 

There was no immobilization of daphnia in the negative control and the limit test concentration of 100 mg/L up to 48 hours exposure. At 0.32 mg/L reference substance, the immobilization of daphnia was 50 and 60 % at 24- and 48-hours exposure, respectively.

 

The analytical test concluded that the test item stable in the test medium up to 49 hours at 1.09 and 109.00 mg/L claimed concentrations. The active ingredient concentration analysis in all test concentrations showed that the per cent agreement with claimed concentration was 75.4% at the start of test and 93.93 % at end of test (48 hours) which is within the acceptable limit.

Hence based on the findings of the study, the EC50 value for FAT 41047/A TE at 48 hours is higher than 100 mg/L.

Description of key information

The EC50 value for FAT 41047/A TE at 48 hours is higher than 100mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

100 mg/L

Additional information

The acute immobilization effect of the test item FAT 41047/A TE was studied on Daphnia magna according to OECD test guideline 202.

 

A range finding test was carried out to determine the test concentration for the definitive test, where Daphnia magna less than 24 hours old were exposed to the test item concentrations of 0.00001, 0.001, 0.01, 0.1, 1.0, 10 and 100 mg/L along with the negative control in test medium (reconstituted water) at20 ± 1 °C. No immolisation was observed at any of the test concentrations during the range finding test.

Based on the results of range finding test, in definitive test,Daphnia magnaless than 24 hours old were exposed to limit test concentrations of100 mg/L along with a negative control and a reference substance, potassium dichromate at 0.32 mg/Lin test medium (reconstituted water) at 20 ± 1 °C. The number of daphnia immobilized was recorded at 24 and 48 hours exposure.

 

There was no immobilization of daphnia in the negative control and the limit test concentration of 100 mg/L up to 48 hours exposure. At0.32 mg/L reference substance, the immobilization of daphnia was 50 and 60 % at 24- and 48-hours exposure, respectively.

 

The analytical test concluded that the test item stable in the test medium up to 49 hours at 1.09 and 109.00 mg/L claimed concentrations. The active ingredient concentration analysis in all test concentrations showed that the per cent agreement with claimed concentration was 75.4% at the start of test and 93.93 % at end of test (48 hours) which is within the acceptable limit.

Hence based on the findings of the study, the EC50 value for FAT 41047/A TE at 48 hours is higher than 100 mg/L.