Carbamic acid, N-[(1R)-2-hydroxy-1-phenylethyl]-, 1,1-dimethylethyl ester

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

02 August 2016 - 10 August 2016

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2016

Report date:

2016

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Carbamic acid, N-[(1R)-2-hydroxy-1-phenylethyl]-, 1,1-dimethylethyl ester

EC Number:

600-273-1

Cas Number:

102089-74-7

Molecular formula:

C13H19NO3

IUPAC Name:

Carbamic acid, N-[(1R)-2-hydroxy-1-phenylethyl]-, 1,1-dimethylethyl ester

Test material form:

solid: particulate/powder

Specific details on test material used for the study:

Batch No LS-53-20150815
Appearance Powder
Aqueous solubility 450mg/L

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

The solution was prepared as follow: 0.1002g of test item were added to 1OOOmL dilution water. The media was mixed for 24 hours at the speed of 500 r/min using a stirrer at 25°C to obtain the 1OOmg/L solution

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

The species to be used in the test is Daplmia mag11a Straus. The daphnia were Laboratory bred, free from overt disease. At the start of the test, the daphnia were aged less than 24 hours.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Post exposure observation period:

24h interval

Test conditions

Test temperature:

20.5-20.8 °C

pH:

7.9

Dissolved oxygen:

8.17-8.29 mg/L

Nominal and measured concentrations:

100mg/l

Details on test conditions:

The limit test was used in the definitive test based on the result of the preliminary test. The solution was prepared as follow: 0.1002g of test item was added to IOOOmL dilution water directly. Then the solution was mixed for 24 hours at the speed of 500 r/min using a stirrer al 25°C to obtain the solution at 1OOmg/L. Blank control was run simultaneously and four replicates with five daphnia in each were carried out for both groups. . The mortalities and immobilization conditions of daphnia were inspected and recorded at 24-hour interval

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Results and discussion

Effect concentrations

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Results with reference substance (positive control):

Potassium dichromate as reference item was tested for EC50 as a means of assuring that the test conditions are reliable at least twice a year in the laboratory. The results of the latest reference item test (Study Number HI185570110, performed on July 04 ~ 05, 2018) before the definitive test showed that EC50-24 h of potassium dichromate to Daphnia magna was 1.22 mg/L, and the 95% confidence limits was 1.06 ~ 1.41 mg/L. The results met the recommended criterion in OECD Guideline No. 202: EC50-24 h of potassium dichromate as results are within the range of 0.6 mg/L to 2.1 mg/L.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

According to test results, the test daphnia were not immobilized or showed any adverse effects in the solution ofN-Boc-D-phenylglycinol at IOOmg/L during 48-hour observation period. Therefore, N-Boc-D-phenylglycinol had no acute immobilization effects on daphnia at 1OOmg/L and the result was expressed as follows:
The 24h EC5o and 48h EC5o were both greater than IOOmg/L

Executive summary:

The  daphnia   sp.  (Daplmia  magna  Straus),   acute   immobilization   test   of N-Boc-D-phenylglycinol wasperformed in thistest.

 The  limit test  was  used  in  the definitive test  based  on  the result  of thepreliminarytest.The solution was prepared as follow: 0.1002goftest item was added to IOOOmLdilution  water directly. Then thesolution was mixed for24 hoursatthespeed of500 r/min using a stirreral25°C toobtain thesolution at 1   OOmg/L. Blankcontrol was run simultaneously and fourreplicateswith five daphnia in each werecarriedoutforbothgroups.

Thetestwascarriedoutusingstaticexposureregime.ThepH,dissolvedoxygenconcentration and temperature were measured at Oh and 48h during the test. Themortalities and immobilization conditions ofdaphnia were inspected and recorded at

24-hourinterval. Concentrationsofthe test item wereanalyzed atthe start andendofthetest.

Accordingto test results, the test daphnia were not immobilized orshowed any

 

adverse effects  in  the solution ofN-Boc-D-phenylglycinol  at IOOmg/L during 48-hour

 

observationperiod. Therefore, N-Boc-D-phenylglycinol had no acute immobilization

effectsondaphnia at \OOmg/Landtheresultwasexpressed asfollows:                                                                 '1

 

The24h EC5oand48h EC5owerebothgreater than I   OOmg/L.