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EC number: 423-860-2 | CAS number: 56309-94-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1996-11-21 till 1996-12-19
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- Version / remarks:
- July 17, 1992
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test)
- Version / remarks:
- December 1992
- Deviations:
- no
- GLP compliance:
- yes
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- sewage, predominantly domestic, non-adapted
- Details on inoculum:
- - Source of inoculum: from the municipal sewage treatment plant Waterschap de Maaskant, `s-Hertogenbosch, the Netherlands
- Pretreatment: The secondary effluent was filtered through a coarse filter paper, the first 200 mL were discarded. The filtrate was kept aerated until inoculation. - Duration of test (contact time):
- 28 d
- Initial conc.:
- 2 mg/L
- Initial conc.:
- 5 mg/L
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: Mineral medium accoding to OECD 301D
- Additional substrate: no
- Test temperature: 20 - 22°C
- pH: 7.5 - 7.6
- pH adjusted: no
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus: 250 - 300 mL BOD bottles with glass stoppers
- Number of culture flasks/concentration: 2
- Measuring equipment: Oxygen meter (WTW: OXI 530, TriOxmatic E0 200)
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes (only inoculum)
- Positive control: yes (reference substance sodium acetat and inoculum)
- Toxicity control: yes (test substance, reference substance and inoculum) - Reference substance:
- benzoic acid, sodium salt
- Test performance:
- The relative biodegradation values calculated from the O2 measurements performed during the test period of 28 days revealed no significant (>10%) degradation of the test item at both concentrations. The oxygen depletion in the toxicity control was always more than 75% of the sum of the oxygen depletion of the positive control and the test substance (low). Therefore, the test item was assumed to be not inhibitory.
Acceptability of the test
Oxygen depletion in the inoculum blank was 0.75 mg O2/L after 28 days. The residual concentration of oxygen in the test bottles was > 0.5 mg/L at any time. The difference of duplicate values expressed as mg O2/L was less than 20% for all duplicate values throughout the incubation period of 28 days. The control substance was degraded by more than 60% within 7 days. - Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 0
- Sampling time:
- 28 d
- Results with reference substance:
- Points of degradation (reference substance):
74 % degradation after 7 d
79 % degradation after 14 d
76 % degradation after 21 d
76 % degradation after 28 d - Validity criteria fulfilled:
- yes
- Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
- The test item is not biodegradable in a test according to OECD 301 D.
- Executive summary:
The test item was tested for its ready biodegradability in the closed bottle test at 2 and 5 mg/L. The study procedure was based on EU Method C.4-E and OECD guideline No. 301 D. The ThOD of the test item (molecular formula: C14H2203, MW= 238.3 g) was calculated to be 2.417 mg O2 per mg. The relative biodegradation values calculated from the O2 measurements performed during the test period of 28 days revealed no significant (0%) degradation at both concentrations. Since all acceptability criteria prescribed by the protocol were met, this study was considered to be valid. In conclusion, the test item was not biodegradable under the conditions in the closed bottle test presently performed.
Reference
Table 1: % Biodegradation at different time points
Test medium |
Concentration (mg/L) |
%Biodegradation after x days |
|||
7 |
14 |
21 |
28 |
||
Positive control* |
2 |
74 |
79 |
76 |
76 |
Test substance low** |
2 |
2 |
0 |
-1 |
-2 |
Test substance high** |
5 |
-1 |
0 |
0 |
-1 |
*ThOD positive control: 0.780 mg O2/mg
**ThOD test substance: 2.417 mg O2/mg
Description of key information
The test item is not biodegradable in a test according to OECD 301 D (reference 5.2.1 -1).
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
- Type of water:
- freshwater
Additional information
The test item was tested for its ready biodegradability in the closed bottle test at 2 and 5 mg/L. The study procedure was based on EU Method C.4-E and OECD guideline No. 301 D. The ThOD of the test item (molecular formula: C14H2203, MW= 238.3 g) was calculated to be 2.417 mg O2 per mg. The relative biodegradation values calculated from the O2 measurements performed during the test period of 28 days revealed no significant (0%) degradation at both concentrations. Since all acceptability criteria prescribed by the protocol were met, this study was considered to be valid. In conclusion, the test item was not biodegradable under the conditions in the closed bottle test presently performed (reference 5.2.1 -1).
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