N-(dodecylphenyl)naphthalen-1-amine

Hydrolysis

Link to relevant study record(s)

Reference

Endpoint:

hydrolysis

Type of information:

experimental study

Adequacy of study:

key study

Study period:

10 May 1999 to 02 December 1999.

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 111 (Hydrolysis as a Function of pH)

Deviations:

not specified

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

No further details specified in the study report.

Radiolabelling:

not specified

Analytical monitoring:

yes

Details on sampling:

Aliquots of the sample solutions were taken from the flasks at various times and the pH of each solution recorded.
The concentration of the sample solution was determined by high performance liquid chromatography (HPLC).

Buffers:

The buffer solutions were filtered through 0.2 μm membrane filters to ensure they were sterile before commencement of the test. Also these solutions were subjected to ultrasonication and degassing with nitrogen to minimise dissolved oxygen content.
Further details tableted see “Any other information”

Details on test conditions:

Preparation of samples
Sample solutions were prepared in stoppered glass flasks at a nominal concentration of 1.50 x 10e-3 g/l in the three buffer solutions (with a 1% acetonitrile co-solvent).
The solutions were shielded from light whilst maintained at the test temperature.

Preliminary Test/Test 1
Sample solutions at pH 4, 7 and 9 were maintained at 50.0 ± 0.5 °C for a period of 120 hours.

Duration:

120 h

pH:

4

Temp.:

50 °C

Initial conc. measured:

0.002 g/L

Duration:

120 h

pH:

7

Temp.:

50 °C

Initial conc. measured:

0.002 g/L

Duration:

120 h

pH:

9

Temp.:

50 °C

Initial conc. measured:

0.002 g/L

Number of replicates:

Duplicate aliquots (A and B) of the test material and each sample solution.

Positive controls:

no

Negative controls:

yes

Statistical methods:

Not specified

Preliminary study:

See "Any other information" for details

Test performance:

The analytical method has been determined to be substance specific and of sufficient accuracy for the test material in this study.

Transformation products:

not specified

Key result

pH:

4

Temp.:

25 °C

DT50:

> 1 yr

Type:

not specified

Key result

pH:

7

Temp.:

25 °C

DT50:

> 1 yr

Type:

not specified

Key result

pH:

9

Temp.:

25 °C

DT50:

> 1 yr

Type:

not specified

Other kinetic parameters:

Not specified

Details on results:

Reported in table form - see "Any other information" for details

Results with reference substance:

Not specified

The mean peak areas relating to the standard and sample solutions

Solution	Mean peak area
Standard 0.749 mg/l Standard 0.801 mg/l Initial Sample A, pH 4 Initial Sample B, pH 4 Initial Sample A, pH 7 Initial Sample B, pH 7 Initial Sample A, pH 9 Initial Sample B, pH 9	16.537 18.519 18.223 16.898 17.371 16.902 16.707 16.448
Standard 0.749 mg/l Standard 0.801 mg/l 2.4 hour Sample A, pH 4 2.4 hour Sample B, pH 4 2.4 hour Sample A, pH 7 2.4 hour Sample B, pH 7 2.4 hour Sample A, pH 9 2.4 hour Sample B, pH 9	15.628 18.513 17.754 17.549 17.151 17.420 15.164 15.231
Standard 0.749 mg/l Standard 0.801 mg/l 24 hour Sample A, pH 4 24 hour Sample B, pH 4 24 hour Sample A, pH 7 24 hour Sample B, pH 7 24 hour Sample A, pH 9 24 hour Sample B, pH 9	16.612 17.163 16.736 15.586 * 15.077 15.680 16.161
Standard 0.749 mg/l Standard 0.801 mg/l 120 hour Sample A, pH 4 120 hour Sample B, pH 4 120 hour Sample A, pH 7 120 hour Sample B, pH 7 120 hour Sample A, pH 9 120 hour Sample B, pH 9	15.031 15.194 14.933 14.791 14.587 15.935 14.249 13.658

* Not used inconsistent peak area (possible dilution error)

 

pH 4 at 50.0±0.5°C

Time (hours)	0	2.4	24	120
Concentration (g/l)	1.55 x 10^-3	1.61 x 10^-3	1.46 x 10^-3	1.48 x 10^-3
% of initial	100	103	94	95

Less than 10% hydrolysis after 5 days at 50°C, equivalent to a half-life of greater than 1 year at 25°C

 

pH 7 at 50.0±0.5°C

Time (hours)	0	2.4	24	120
Concentration (g/l)	1.52 x 10^-3	1.57 x 10^-3	1.36 x 10^-3	1.52 x 10^-3
% of initial	100	104	90	100

Less than 10% hydrolysis after 5 days at 50°C, equivalent to a half-life of greater than 1 year at 25°C

 

pH 9 at 50.0±0.5°C

Time (hours)	0	2.4	24	120
Concentration (g/l)	1.47 x 10^-3	1.38 x 10^-3	1.44 x 10^-3	1.39 x 10^-3
% of initial	100	94	98	95

Less than 10% hydrolysis after 5 days at 50°C, equivalent to a half-life of greater than 1 year at 25°C

Validity criteria fulfilled:

yes

Conclusions:

The results for all pH levels tested, 4, 7 and 9, was less than 10% hydrolysis after 5 days at 50 °C equivalent to a half-life of greater than 1 year at 25 °C.

Executive summary:

The hydrolysis of APAN as a function of pH has been determined.

The method employed complied with that specified in Method 11 1 of the OECD Guidelines for Testing of Chemicals, 12 May 1981.

 

The results for all pH levels tested, 4, 7 and 9, was less than 10% hydrolysis after 5 days at 50 °C equivalent to a half-life of greater than 1 year at 25 °C.

Description of key information

The hydrolysis of APAN as a function of pH has been determined in a GLP accredited laboratory study in accordance with OECD Guideline No. 111.

Key value for chemical safety assessment

Half-life for hydrolysis:

1 yr

at the temperature of:

25 °C

Additional information

The hydrolysis of APAN as a function of pH has been determined.

 

The results for all pH levels tested, 4, 7 and 9, was less than 10% hydrolysis after 5 days at 50 °C equivalent to a half-life of greater than 1 year at 25 °C.