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EC number: 200-059-4 | CAS number: 50-69-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- December 1999
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 999
- Report date:
- 1999
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- D-ribose
- EC Number:
- 200-059-4
- EC Name:
- D-ribose
- Cas Number:
- 50-69-1
- Molecular formula:
- C5H10O5
- IUPAC Name:
- D-ribose
- Test material form:
- solid: particulate/powder
- Details on test material:
- Dry powder, white to slightly yellow
Constituent 1
Method
- Target gene:
- HIS, rfa, uvrB, R-factor
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Additional strain / cell type characteristics:
- other: HIS G46/C3076/D3052
- Species / strain / cell type:
- E. coli WP2 uvr A
- Additional strain / cell type characteristics:
- other: Trp
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9
- Test concentrations with justification for top dose:
- 0; 62; 185; 556; 1667; 5000 µg/L
top dose in accordance to test guideline in the absence of cytotoxicity. - Vehicle / solvent:
- Water
Controls
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- 2-nitrofluorene
- sodium azide
- benzo(a)pyrene
- ethylnitrosurea
- other: 2-aminoanthracene, 2.0µg/plate
Results and discussion
Test results
- Key result
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
Any other information on results incl. tables
positive control mutagens |
||
strain |
in the absence of the S9-mix |
in the presence of the S9-mix |
TA 1535 |
sodium azide: 1.0 tg/plate |
2-aminoanthraTéene: 2.0 ug,,Iplate |
TA 1537 |
9-aminoacridine: 801.14/plate |
benzo(a)pyrene: 4.0 ..tg/plate |
TA 98 |
2-nitrofluorene: 2.0 µg/plate |
2-aminoanthracene: 2.0 µg/plate |
TA 100 |
sodium azide: 1.0 µg/plate |
2-aminoanthracene: 2.0 ug/91ate |
WP2uvrA |
N-ethyl-N-nitrosourea: 100 gg/plate |
2-aminoanthracene: 80 µg/plate |
Dose | TA1535 | TA1537 | TA98 | TA100 | e.coli | ||||||
µg/L | S9- | S9+ | S9- | S9+ | S9- | S9+ | S9- | S9+ | S9- | S9+ | |
16 | 14 | 14 | 15 | 22 | 46 | 121 | 128 | 27 | 23 | ||
13 | 12 | 16 | 15 | 29 | 34 | 121 | 129 | 28 | 24 | ||
17 | 16 | 9 | 19 | 26 | 36 | 155 | 127 | 22 | 33 | ||
0 | Mean | 15 | 14 | 13 | 16 | 26 | 39 | 132 | 128 | 26 | 27 |
Sd | 2 | 2 | 4 | 2 | 4 | 6 | 20 | 1 | 3 | 6 | |
22 | 8 | 9 | 11 | 20 | 46 | 126 | 131 | 20 | 19 | ||
14 | 11 | 17 | 9 | 31 | 45 | 143 | 143 | 26 | 28 | ||
17 | 14 | 11 | 17 | 22 | 36 | 128 | 118 | 13 | 24 | ||
62 | Mean | 18 | 11 | 12 | 12 | 24 | 42 | 132 | 131 | 20 | 24 |
Sd | 4 | 3 | 4 | 4 | 6 | 6 | 9 | · 13 | 7 | 5 | |
22 | 6 | 9 | 12 | 27 | 28 | 126 | 121 | 27 | 27 | ||
18 | 8 | 16 | 18 | 33 | 40 | 122 | 104 | 31 | 19 | ||
18 | 10 | 13 | 19 | 35 | 45 | 132 | 123 | 37 | 18 | ||
185 | Mean | 19 | 8 | 13 | 16 | 32 | 38 | 127 | 116 | 32 | 21 |
Sd | 2 | 2 | 4 | 4 | 4 | 9 | 5 | 10 | 5 | 5 | |
19 | 11 | 11 | 16 | 25 | 37 | 122 | 143 | 19 | 28 | ||
20 | 12 | 12 | 17 | 14 | 27 | 100 | 121 | 26 | 29 | ||
14 | 22 | 18 | 20 | 24 | 42 | 126 | 124 | 25 | 26 | ||
556 | Mean | 18 | 15 | 14 | 18 | 21 | 35 | 116 | 129 | 23 | 28 |
Sd | 3 | 6 | 4 | 2 | 6 | 14 | 12 | 4 | 2 | ||
22 | 14 | 17 | 15 | 25 | 58 | 122 | 129 | 28 | 36 | ||
12 | 9 | 8 | 16 | 38 | 31 | 132 | 132 | 25 | 19 | ||
17 | 6 | 9 | 24 | 33 | 35 | 118 | 138 | 27 | 22 | ||
1667 | Mean | 17 | 10 | 11 | 18 | 32 | 41 | 124 | 133 | 27 | 26 |
Sd | 5 | . 4 | 5 | 5 | 7 | 15 | 7 | 5 | 2 | 9 | |
13 | 7 | 8 | 18 | 19 | 52 | 99 | 124 | 22 | 20 | ||
21 | 9 | 8 | 7 | 19 | 46 | 126 | 140 | 23 | 22 | ||
14 | 12 | 9 | 9 | 27 | 41 | 141 | 139 | 30 | 24 | ||
5000 | Mean | 16 | 9 | 8 | 11 | 22 | 46 | 122 | 134 | 25 | 22 |
Sd | 4 | 3 | 1 | 6 | 5 | 6 | 21 | 9 | 4 | 2 | |
387 | 212 | 769 | 270 | 888 | 1091 | 406 | 1577 | 212 | 944 | ||
398 | 216 | 707 | 219 | 873 | 1052 | 423 | 1453 | 173 | 950 | ||
411 | 222 | 633 | 192 | 980 | 981 | 397 | 1485 | 194 | 1049 | ||
Pos.C. | Mean | 399 | 217 | 703 | 227 | 914 | 1041 | 409 | 1505 | 193 | 981 |
Sd | 12 | 5 | 68 | 40 | 58 | 56 | 13 | 64 | 20 | 59 |
Applicant's summary and conclusion
- Conclusions:
- It is concluded that the results obtained with the test substance D-Ribose in Salmonella typhimurium strains TA 1535, TA 1537, TA 98 and TA 100, and in the Escherichia coli strain WP2 uvrA, in both the absence and the presence of the S9-mix, indicate that D-Ribose was not mutagenic under the conditions employed in this study.
- Executive summary:
The results of the mutagenicity assays are shown in Table 1 and 2.
Two independent assays were performed with all strains in both the absence and the presence of the S9-mix with five different concentrations of the test substance, ranging from 62 -5000 lag/plate (first assay) and 313 -5000 µg/plate (second assay). The test compound was dissolved in water. Negative controls (solvent) and positive controls were run simultaneously with the test substance.
A slight decrease in the mean number of revertant colonies observed in some strains in the second test indicates possible toxicity to these strains by the test substance D-Ribose.
In both assays, in the absence and the presence of the S9-mix and in all strains, D-Ribose did not cause a two-fold or greater increase in the mean number of revertant colonies appearing in the test plates compared to the background spontaneous reversion rate observed with the negative control, and did not give evidence of a dose-response relationship.
The mean number of his + and trp+ revertant colonies of the negative controls were within the acceptable range, and the positive controls gave the expected increase in the mean number of revertant colonies.
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