Phosphonium, trihexyltetradecyl-, chloride

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Data is from peer reviewed journal. Study conducted according to standard methodology. As no analytical monitoring has been performed, the concentration tested cannot be checked. Therefore, this study is considered as reliable with restrictions.

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

(2004)

Deviations:

no

GLP compliance:

not specified

Remarks:

The information available in the publication does not indicate whether or not the laboratory conducted the study under GLP conditions.

Specific details on test material used for the study:

Composition of test material, percentage of components: Trihexyl(tetradecyl)phosphonium chloride (258864-54-9), 96.4%

Analytical monitoring:

not specified

Vehicle:

no

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Strain: Daphnia magna Straus 1820 (Cladocera crustacea)
- Age at study initiation (mean and range, SD): <24 h old
- Feeding during test: Not fed
- Method of breeding: continuous and balanced breeding
- Culture water: 'M4 medium', partly renewed once a week

Test type:

not specified

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

No data

Test temperature:

20 +/- 2°C

pH:

No data

Dissolved oxygen:

No data

Salinity:

Not applicable

Conductivity:

No data

Nominal and measured concentrations:

Nominal concentrations of test material ranged from 0.03 to 1.0 mg/L. These concentrations were selected according a preliminary range-finding screening test.

Details on test conditions:

TEST SYSTEM
- Test vessel: glass test tubes filled with 10 mL of test solution
- Aeration: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 2 mL for each daphnid

OTHER TEST CONDITIONS
- Photoperiod: All the tests were conducted in darkness

Reference substance (positive control):

yes

Remarks:

potassium dichromate (K2Cr2O7)

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

0.053 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

EC50 value is derived from the concentration-response curve using a logistic Hill model . The 95% confidence intervals was estimated using a “bootstrap” simulation method.
EC50 48 h = 0.053 mg/L (95% IC: 0.046–0.054)

Validity criteria fulfilled:

not specified

Conclusions:

Trihexyltetradecylphosphonium chloride was very toxic to daphnids, with a reported EC50 value of 0.053 mg/L (nominal concentration).

Executive summary:

In this study, the acute toxicity of the test substance (Trihexyltetradecylphosphonium chloride) to Daphnia magna was determined in a 48 hour test according to the OECD Guideline No. 202 (2004).

 

The test substance concentrations, selected for the main test, were based on a preliminary range-finding screening test and ranged from 0.03 to 1.0 mg/L. Stock solutions were prepared by introducing directly the required amount of the selected compound in dilution water. 20 animals were tested per concentration. Observations were made on the immobilization rate.

 

In the condition of this study, a 48h EC50 of 0.053 mg/L is obtained. This result is expressed as nominal concentration since no analytical measurement has been performed.

Description of key information

The acute toxicity of Trihexyltetradecylphosphonium chloride to Daphnia magna was investigated in a 48 hour test according to the OECD Guideline No. 202 (2004).

The test substance concentrations, selected for the main test, were based on a preliminary range-finding screening test and ranged from 0.03 to 1.0 mg/L. Stock solutions were prepared by introducing directly the required amount of the selected compound in dilution water. 20 animals were tested per concentration. Observations were made on the immobilization rate.

In the condition of this study, a 48h EC50 of 0.053 mg/L is obtained. This result is expressed as nominal concentration since no analytical measurement has been performed.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

0.053 mg/L