Fatty acids, rape-oil, hydrogenated, reaction products with diethylenetriamine, di-Me sulfate-quaternized

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

July 22, 2010

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

yes

Remarks:

Respirometer

Details on sampling:

- Measurement for each group, i.e. test substance, negative and positive controls
- After 10 min

Vehicle:

no

Details on test solutions:

Stock solution: 1.0 mg/mL in water and the appropriate amount was directly added in the test solution

Test organisms (species):

activated sludge of a predominantly domestic sewage

Details on inoculum:

Source: Warcham Waste Water Treatment Plant, Warchal, Massachussets
Solid contents: 4.1 g/L

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

3 h

Remarks on exposure duration:

coninuously aerated

Test temperature:

20.6 - 20.8

pH:

6.88 -7.27

Salinity:

-

Conductivity:

-

Nominal and measured concentrations:

0 (negative control), 0.1, 1.0, 10, 100 and 1000 mg/L

Details on test conditions:

Final volume of 500 mL

Reference substance (positive control):

yes

Remarks:

3,5-dichlorophenol

Key result

Duration:

3 h

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Details on results:

Control vessels: 15.9 and 16.8 mg O2/L/hour.
Test substance vessels at concentrations of 0.1, 1.0, 10, 100 and 1000 mg/L: 17.2, 15.3, 18.0, 16.4 and 18.5 mg O2/L/hour, respectively
No differences were observed between the control and the test conditions

Results with reference substance (positive control):

Positive control vessels at concentrations of 1.0, 3.0, 10 and 30 mg/L: 10.8, 11.0, 5.7 and 1.8 mg O2/L/hour, respectively
EC50 value of 5.7 mg a.i./L

Validity criteria fulfilled:

yes

Conclusions:

Under the study conditions, the 3 h EC50 (in activated sludge) of the test substance was determined to be >1000 mg/L (nominal).

Executive summary:

A study was conducted to determine the toxicity of the test substance, 'di-C18-22 AAEMIM-MS' (active: 100%), to microorganisms according to OECD Guideline 209 (Activated Sludge Respiration Inhibition Test), in compliance with GLP. Approximately 4 g/L of activated sludge (domestic) was exposed to the test substance at concentrations of 0 (negative control), 0.1, 1.0, 10, 100 and 1000 mg/L for 3 h. The dissolved oxygen (measurement of oxygen depletion rates or oxygen consumption) was recorded using a respirometer. A positive control group (3,5 -dichlorophenol) was included in the experiment and gave values within the expected range. In the control vessels, the oxygen consumption was 15.9 and 16.8 mg O2/L/h and, in the test substance vessels at concentrations of 0.1, 1.0, 10, 100 and 1000 mg/L, it was 17.2, 15.3, 18.0, 16.4 and 18.5 mg O2/L/h, respectively. No statistical differences were observed between the negative controls and the test groups. Under the study conditions, the 3 h EC50 (in activated sludge) of the test substance was determined to be >1000 mg/L (Springborn, 2010). No NOEC was reported by the author, however considering the absence of toxicity at any dose level, the NOEC can be assumed to be 1000 mg/L (nominal).

Description of key information

Based on the results of the study, 3 h EC50 (in activated sludge) of the test substance, 'di-C18-22 AAEMIM-MS', to microorganisms, was determined to be >1000 mg/L (nominal).

Key value for chemical safety assessment

EC50 for microorganisms:

1 000 mg/L

Additional information

A study was conducted to determine the toxicity of the test substance, 'di-C18-22 AAEMIM-MS' (active: 100%), to microorganisms according to OECD Guideline 209 (Activated Sludge Respiration Inhibition Test), in compliance with GLP. Approximately 4 g/L of activated sludge (domestic) was exposed to the test substance at concentrations of 0 (negative control), 0.1, 1.0, 10, 100 and 1000 mg/L for 3 h. The dissolved oxygen (measurement of oxygen depletion rates or oxygen consumption) was recorded using a respirometer. A positive control group (3,5 -dichlorophenol) was included in the experiment and gave values within the expected range. In the control vessels, the oxygen consumption was 15.9 and 16.8 mg O2/L/h and, in the test substance vessels at concentrations of 0.1, 1.0, 10, 100 and 1000 mg/L, it was 17.2, 15.3, 18.0, 16.4 and 18.5 mg O2/L/h, respectively. No statistical differences were observed between the negative controls and the test groups. Under the study conditions, the 3 h EC50 (in activated sludge) of the test substance was determined to be >1000 mg/L (Springborn, 2010)