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EC number: 277-531-1 | CAS number: 73545-11-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 21 October - 24 October 2019
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 020
- Report date:
- 2020
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 13 April 2004
- Deviations:
- yes
- Remarks:
- no acclimation period of 48hrs was performed. Deviation considered uncritical
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 7-(4-ethyl-1-methyloctyl)quinolin-8-ol
- EC Number:
- 277-531-1
- EC Name:
- 7-(4-ethyl-1-methyloctyl)quinolin-8-ol
- Cas Number:
- 73545-11-6
- Molecular formula:
- C20H29NO
- IUPAC Name:
- 7-(5-ethylnonan-2-yl)quinolin-8-ol
- Test material form:
- liquid
Constituent 1
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Sponsor / 1702-18-01/O
- Expiration date of the lot/batch: 25 March 2021
- Purity test date: 26 March 2018
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Room Temperature (20 ± 5 °C); Keep away from light. The test item was stored in a closed vessel dark and dry at room temperature (16.2 – 23.7 °C).
- Solubility and stability of the test substance in the solvent/vehicle:
Stability: H2O: unknown; Ethanol: unknown; acetone; unknown; CH3CN: unknown; DMSO: unknown
Solubility: H2O: < 0.1 g/L; Ethanol: unknown; acetone: > 1 g/L; CH3CN: unknown; DMSO: unknown
TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing:
A saturated solution was prepared for the test. This was done by mixing the real load of 99.3 mg/L resp. 100.0 µL/L test item (based on a density of 0.9926 g/mL determined in LAUS study 18081404G912) with the corresponding amount of dilution water and stirring for 24 hours on a magnetic stirrer. The test item was pipetted directly onto the surface of the test dilution water. The lower phase of the resulting solution was used unfiltrated.
The lower treatments (4.6 / 10 / 22 / 46 mg/L nominal) were prepared by dilution of this solution with dilution water.
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A saturated solution was prepared for the test. This was done by mixing the real load of 99.3 mg/L resp. 100.0 µL/L test item (based on a density of 0.9926 g/mL determined in LAUS study 18081404G912) with the corresponding amount of dilution water and stirring for 24 hours on a magnetic stirrer. The test item was pipetted directly onto the surface of the test dilution water. The lower phase of the resulting solution was used unfiltrated.
The lower treatments (4.6 / 10 / 22 / 46 mg/L nominal) were prepared by dilution of this solution with dilution water.
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): No
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Strain/clone: STRAUS
- Age at study initiation (mean and range, SD): 0-24 hours
- Method of breeding: In-house breeding since 2007
- Source: Origin: Umweltbundesamt Berlin
- Age of parental stock (mean and range, SD):
- Feeding during test
- Food type: green algae (Desmodesmus subspicatus)
Animal Husbandry
Daphnia magna is bred in the LAUS GmbH throughout the year. The animals are kept for the use in toxicity tests. They multiply by parthenogenesis, thus being genetically identi-cal. The keeping is performed similar to the method described in the OECD guideline, fol-lowing SOP 115 002 01 („Zucht und Hälterung von Daphnia magna STRAUS“), version 12 from 02. Feb. 2015.
Vessels preserving glasses, nominal volume 2 L
Medium M4-Medium (recipe of ELENDT)
Food green algae (Desmodesmus subspicatus)
Medium renewal twice a week
Photo period 16/8 hours, using neon tubes
Temperature 20 +/- 2 °C
ACCLIMATISATION:
22 hours and 40 minutes before the start of the test, the adult animals were separated from the young. 40 minutes before test start, the adults were caught with the help of a glass tube, and the newborn daphnia (age < 24 h) were sieved from the medium and im-mediately placed into a beaker containing dilution water. After the settling-in period, ani-mals which showed no apparent damage were used for the test.
Switching from M4-medium (husbandry) to Dilution water (test) has been shown not to cause any detrimental effects for test daphnia, therefore no acclimation period of 48 h was performed. This is a deviation from OECD Guideline No. 202 and EU-Method C.2, which can be considered uncritical
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Post exposure observation period:
- No
Test conditions
- Test temperature:
- 19.1 – 20.3 °C
- pH:
- Reported in the table below .
- Dissolved oxygen:
- Reported in the table below.
- Salinity:
- N/A
- Nominal and measured concentrations:
- 4.6 / 10 / 22 / 46 / 100 mg/L (nominal concentration)
The concentrations to be tested are based on the result of a non-GLP pre-test. - Details on test conditions:
- TEST SYSTEM
- Test vessel: glass beakers, nominal volume 50 mL, tall shape
- Type (delete if not applicable): open
- Renewal rate of test solution (frequency/flow rate): No renewal
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4 vessels, each containing 20 ± 5 mL test solution and 5 daphnia
- No. of vessels per vehicle control (replicates): 4 vessels, each containing 20 ± 5 mL dilution water and 5 daphnia
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Dilution water (Daphnia medium) with the following specification was used (stated in SOP 11800201)
Parameter Concentration in mg/L
CaCl2*2H2O 293.80
MgSO4*7H2O 123.30
NaHCO3 64.80
KCl 5.80
Deviations from the nominal weighted loads were less than 5 %. Exact values are record-ed in the raw data. After preparation, the dilution water was aerated and the pH was measured. The pH was 7.7.
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod:
- Light intensity:
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
VEHICLE CONTROL PERFORMED: yes/no
RANGE-FINDING STUDY
- Test concentrations:
- Results used to determine the conditions for the definitive study: - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate K2Cr2O7 (CAS No. 7778-50-9) was used as positive control in a current reference study (201902R201).
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 92.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 46 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Results with reference substance (positive control):
- The 24h-EC50 value was determined as 1.98 mg/L. The value was within the requested range for the 24h-EC50 between 0.6 - 2.1 mg/L of potassium dichromate K2Cr2O7 as speci-fied in the OECD guideline.
- Reported statistics and error estimates:
- The estimation of the biological results was accomplished using the software ToxRat® Professional, version 3.3.0. The details of calculation are stated in the Annex 4: Statistical calculation using ToxRat® Professional 3.3.0.
Any other information on results incl. tables
Findings
Immobility
In the blank control and the lower treatments (4.6 – 46 mg/L), none of the daphnia was immobilised (see table below).
Table 1. Immobility
Nominal Concentration in mg/L |
Immobility 24 hours |
Immobility 48 hours |
||||||||
absolute |
in % |
absolute |
in % |
|||||||
Blank control |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
4.6 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
10 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
22 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
46 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
100 |
2 |
3 |
2 |
1 |
40 |
3 |
5 |
3 |
3 |
70 |
pH and O2
The pH values and the concentration of dissolved oxygen in the test media and the blank control are given in the following table:
Table 2.pH and O2-values
Nominal Concentration in mg/L |
pH |
O2-Concentration in mg/L |
||
0 h |
48 h |
0 h |
48 h |
|
Blank control |
7.7 |
7.8 |
8.2 |
8.0 |
4.6 |
7.8 |
7.8 |
8.2 |
8.1 |
10 |
7.7 |
7.7 |
8.3 |
8.1 |
22 |
7.8 |
7.7 |
8.2 |
8.0 |
46 |
7.7 |
7.7 |
8.2 |
8.1 |
100 |
7.8 |
7.7 |
8.0 |
8.2 |
Analytical Determinations
At the start and at the end of the test, the content of the test item in the test solutions was estimated by determination of the total organic carbon (TOC) content in the test solutions using a carbon analyser.
Because of the very low solubility of the test item, the measured concentrations in the test solutions were in a similar low range as in the control.
The details are given in the following tables:
Table3.Measured Concentrations IC and TC
Nominal |
Measured TC |
Measured TC |
Measured IC |
Measured IC |
mg/L |
mg/L |
mg/L |
mg/L |
mg/L |
Blank control |
10.43 |
11.25 |
9.90 |
9.47 |
4.6 |
10.55 |
10.89 |
9.85 |
9.59 |
10 |
10.50 |
11.73 |
9.58 |
9.53 |
22 |
9.76 |
11.07 |
9.73 |
9.31 |
46 |
10.15 |
11.18 |
9.90 |
9.38 |
100 |
10.89 |
11.61 |
9.52 |
9.39 |
LOQ (Limit of quantification) TC = 0.90 mg/L
LOQ (Limit of quantification) IC = 1.59 mg/L
Table 4. Calculated Concentrations TOC
Nominal |
Calculated TOC (TC-IC) |
Calculated TOC (TC-IC) |
mg/L |
mg/L |
mg/L |
Blank control |
0.52 |
1.78 |
4.6 |
0.71 |
1.30 |
10 |
0.92 |
2.20 |
22 |
0.03 |
1.76 |
46 |
0.25 |
1.80 |
100 |
1.37 |
2.21 |
Biological Results Test Item
The biological results are presented in the following table:
Table 5. Biological Results Test Item
Parameter |
Value |
95%-confidence interval |
24h EC50 |
> 100 mg/L |
not determinable |
48h EC50 |
92.2 mg/L |
not determinable |
48h NOEC |
46 mg/L |
-- |
48h LOEC |
100 mg/L |
-- |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- One valid experiment was performed.
20 Daphnia were exposed to the test item for the treatment for 48 hours in a static test system.
Only the highest concentrated treatment showed toxicity of 70 % immobilisation. None of the animals were immobilised in the blank control and the lower treatments.
Potassium dichromate K2Cr2O7 (CAS No. 7778-50-9) was used as positive control in a current reference study to assure that the test conditions are reliable.
At the start and at the end of the test, the content of the test item in the test solutions was estimated by determination of the total organic carbon (TOC) content in the test solutions using a carbon analyser.
Because of the very low solubility of the test item, the measured concentrations in the test solutions were in a similar low range as in the control.
Therefore, calculation of a mean exposure concentration of dissolved test item was not possible, and the determination of the biological results was based on the nominal concentration (OECD Guidance Doc. No.23, §176).
However strong toxicity (70% immobility) in the highest concentrated treatment 100 mg/L showed the presence of dissolved test item in the test solution.
No observations were made which might cause doubts concerning the validity of the study outcome. All validity criteria were met.
The result of the test is considered valid. - Executive summary:
One valid experiment was performed.
The study was performed using 5 concentrations ranging from 4.6 to 100 mg/L nominal concentration. For each test concentration and the blank control, 20 daphnia were exposed to the test item for 48 hours in a static test system. After 24 and 48 hours, the immobilised daphnia were counted.
Only the highest concentrated treatment (100 mg/L nominal concentration) showed toxicity of 70 % immobilisation. None of the animals was immobilised in the blank control and the lower treatments.
Potassium dichromate K2Cr2O7(CAS No. 7778-50-9) was used as positive controlin a current reference study to assure that the test conditions are reliable.
At the start and at the end of the test, the content of the test item in the test solutions was estimated by determination of the total organic carbon (TOC) content in the test solutions using a carbon analyser.
Because of the very low solubility of the test item, the measured concentrations in the test solutions were in a similar low range as in the control.
Therefore, calculation of a mean exposure concentration of dissolved test item was not possible, and the determination of the biological results was based on the nominal concentration (OECD Guidance Doc. No.23, §176).
However strong toxicity (70% immobility) in the highest concentrated treatment 100 mg/L showed the presence of dissolved test item in the test solution.
The following results were determined for the test item7-(4-ethyl-1-methyloctyl)quinolin-8-ol (species:Daphnia magna).48h-NOEC=46 mg/L (nominal)
48h-LOEC =100mg/L (nominal)
24h-EC50> 100 mg/L(nominal)
48h-EC50= 92.2 mg/L(nominal)
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