Cinchocaine

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2018

Report date:

2018

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

1

Reference substance name:

Cinchocaine

EC Number:

201-632-1

EC Name:

Cinchocaine

Cas Number:

85-79-0

Molecular formula:

C20H29N3O2

IUPAC Name:

2-butoxy-N-[2-(diethylamino)ethyl]quinoline-4-carboxamide

Test material form:

solid: particulate/powder

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Because the test item is poorly soluble in water, a test solution was prepared using a saturated solution method according to the Guidance Document on Aquatic Toxicity Testing of Difficult Substances and Mixtures, OECD No. 23.

Test solutions

Vehicle:

yes

Remarks:

Reconstituted algal growth medium (OECD medium)

Test organisms

Test organisms (species):

Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)

Study design

Test type:

semi-static

Water media type:

other: Reconstituted algal growth medium (OECD medium)

Limit test:

no

Total exposure duration:

72 h

Test conditions

Test temperature:

between 22.2 and 23.1 °C

pH:

range of the pH was 7.54 – 8.99

Details on test conditions:

The exposure time was 72 hours. The test was started (0 hours) by inoculation of a biomass of approximately 104 algal cells per mL test medium.

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

72 h

Dose descriptor:

EC50

Remarks:

Growth

Effect conc.:

1.11 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 2

Key result

Duration:

72 h

Dose descriptor:

EC50

Remarks:

Yield

Effect conc.:

0.53 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

other: yield

Effect concentrations 3

Key result

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

0.62 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

biomass

Effect concentrations 4

Key result

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

0.17 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 5

Key result

Duration:

72 h

Dose descriptor:

LOEC

Effect conc.:

0.52 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

growth rate

Results with reference substance (positive control):

The 72h ErC 50: 0.99 mg/L, (95 % confidence limits: 0.91 – 1.07 mg/L)
The 72h EbC 50: 0.69 mg/L, (95 % confidence limits: 0.64 – 0.75 mg/L)
The 72h EyC 50: 0.60 mg/L, (95 % confidence limits: 0.55 – 0.65 mg/L)
These values are within the range of laboratory ring test data

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

With respect to the inhibitory effect of the test item, the 0-72 h average specific growth rates and areas were significantly different from that of the control group at the examined concentration range of 0.52 – 5.79 mg/L (measured), therefore the NOEC was determined as 0.17 mg/L (measured) and LOEC was determined as 0.52 mg/L (measured). The 0-72 h yield were significantly different from that of the control group in the examined concentration range of 0.17 – 5.79 mg/L (measured). The NOEC was determined as 0.06 mg/L (measured); the LOEC was determined as 0.17 mg/L (measured).

Executive summary:

The toxicity potential on algae was performed according to OECD201 guideline. The resultats show that the inhibitory effect of the test item, the 0-72 h average specific growth rates and areas were significantly different from that of the control group at the examined concentration range of 0.52 – 5.79 mg/L (measured), therefore the results based on growth rate were :

- NOEC = 0.17 mg/L (measued)

- LOEC = 0.52 mg/L (measured)

- CE50 = 1.1 mg/L (measured)