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EC number: 814-615-4 | CAS number: 122958-50-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2019
- Reliability:
- 1 (reliable without restriction)
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- yes (incl. QA statement)
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Total exposure duration:
- 3 h
- Remarks on exposure duration:
- contact time 3 hours, during which aeration takes place
- Test temperature:
- 20 ± 2°C
- Details on test conditions:
- Duration: contact time 3 hours, during which aeration takes place
Air supply: clean, oil-free air. Air flow 0.5 to 1 litre/minute
Nutrient solution: synthetic sewage feed
Test item concentrations:: 0.10, 1.0, 10.0, 100.0 and 1000.0 mg/l
Reference item concentrations: 0.01 , 0.1, 1.0 , 10.0 and 100.0 mg/l
Blank controls: Inoculated sample without test substance
Temperature: 20 ± 2°C
Test item stock solutions 10 g/l (at pH = 7.0) (no undissolved particles were
observed)
Reference substance stock solutions 1 g/l
Synthetic medium stock solution: See p. 3.1.4
Activated sludge stock suspension: 3 g/l of suspended solids
Activated sludge suspension in test vessels 1.5 g/l of suspended solids
ATU stock solution 2.32 g/l - Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- 0.43 mg/L
- Basis for effect:
- inhibition of total respiration
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- 0.19 mg/L
- Basis for effect:
- inhibition of heterotrophic respiration
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- 0.68 mg/L
- Basis for effect:
- inhibition of nitrification rate
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Remarks:
- extrapolated
- Effect conc.:
- 2 796 mg/L
- Basis for effect:
- inhibition of nitrification rate
- Details on results:
- The lowest tested concentration that causes the observed respiration inhibition is equal to 0.1 mg/l.
The test item – in the measured substance content range of 0.1-1000 mg/l - has a toxic effect on the oxidation of ammonium (nitrification) but only slight, with tendency to diminish for increasing test item concentration, on heterotrophic oxidation (biodegradation).
The heterotrophic respiration was stimulated (probably hormesis phenomenon), achieving about 0% (extrapolated) of inhibition for test item content greater than 1000 mg/l. - Validity criteria fulfilled:
- yes
- Conclusions:
- calculated values of the end points EC10 for the test item were found to be:
1. Total respiration inhibition: 0.43 mg/l.
2. Heterotrophic respiration inhibition: 0.19 mg/l.
3. Nitrification inhibition: 0.68 mg/l .
The lowest tested concentration that causes the observed respiration inhibition is equal to 0.1 mg/l. The very low doses of test item did not influence heterotrophic respiration process (biodegradation, i.e. organic carbon oxidation) up to 1 mg/l. For higher concentrations heterotrophic respiration increased and instead of respiration inhibition we observed a stimulation of oxygen consumption. May be a hormesis phenomenon was revealed.
Hormesis is any process in a cell or organism that exhibits a biphasic response to exposure to increasing amounts of a substance. In toxicology, hormesis is a dose response phenomenon characterized by a low dose stimulation, high dose inhibition. This hormesis process did not influenced a nitrification process. The test substance has a toxic effect on the nitrifying bacteria, oxidising ammonium to nitrite and nitrate. Even very low doses (10 mg/l) influenced nitrification process and caused an inhibition of this process.
For higher substance content a nitrification inhibition increased up to 41.8 % for substance concentration equel to 1000 mg/l. The test item – in the measured content range - has a toxic effect on the oxidation of ammonium (nitrification) but no or slight one on heterotrophic oxidation (biodegradation). The test item – in the measured substance content range of 0.1-1000 mg/l - has a toxic effect on the oxidation of ammonium (nitrification) but only slight, with tendency to diminish for increasing test item concentration, on heterotrophic oxidation (biodegradation).
the heterotrophic respiration was stimulated (probably hormesis phenomenon), achieving about 0% (extrapolated) of inhibition for test item content greater than 1000 mg/l.
Reference
Description of key information
Key value for chemical safety assessment
- EC50 for microorganisms:
- 2 796 mg/L
- EC10 or NOEC for microorganisms:
- 0.19 mg/L
Additional information
calculated values of the end points EC10 for the test item were found to be:
1. Total respiration inhibition: 0.43 mg/l.
2. Heterotrophic respiration inhibition: 0.19 mg/l.
3. Nitrification inhibition: 0.68 mg/l .
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