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EC number: 265-116-8 | CAS number: 64742-16-1 A complex combination of organic compounds, predominantly hydrocarbons, obtained as a fraction of the extract from solvent extraction of residuum. It consists predominantly of high molecular weight compounds with high carbon-to-hydrogen ratios.
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 16. Apr. 2018 to 22. Jun. 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- Version / remarks:
- OECD Guideline for the Testing of Chemicals No. 201, adopted 23. Mar. 2006, Annex 5 corrected: 28 July 2011 “Freshwater Alga and Cyanobacteria, Growth Inhibition Test”
- Deviations:
- yes
- Remarks:
- See "Any other information" for details
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Version / remarks:
- Regulation EU No. 2016/266 amending Regulation EC No. 440/2008, Annex IV, Method C.3: “FRESHWATER ALGAE AND CYANOBACTERIA, GROWTH INHIBITION TEST,” adopted 07. December 2015
- Deviations:
- yes
- Remarks:
- See "Any other information" for details
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- No further details specified in the study report.
- Analytical monitoring:
- yes
- Details on sampling:
- The real cell concentration at the beginning of each test was measured with an electronic particle counter in the blank control solution. This measured value was used as start cell concentration for all replicates.
From these mixtures samples for the analytical determination were taken, afterwards the pH-value was measured. - Vehicle:
- no
- Details on test solutions:
- A water-accommodated fraction (WAF) was prepared for the test. This was done by weighing each nomi-nal load (1 / 3.2 / 10 / 32 / 100 mg/L), adding the corresponding amounts of algal medium (demineralised water enriched with minerals but without algae) and shaking vigorously for 23 hours (experiment I), respectively 24 hours (experiment II). The resulting solutions were filtrated through 0.45 µm PTFE filters.
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- Specification: Unicellular freshwater green alga.
Genus: Desmodesmus
Species: subspicatus
SAG Strain Number: 86.81
Taxonomic position: Chlorophyta - Chlorophyceae
Origin and Culture
The culture of Desmodesmus subspicatus was obtained in January 2016 by MBM Sciencebridge GmbH (Institut für Pflanzenphysiologie of Universität Göttingen). The algae are kept as stock culture on solid agar at 2 - 8 °C. From the stock culture, a permanent culture was prepared. From an aliquot of the permanent culture, the pre-culture was prepared. - Test type:
- not specified
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Post exposure observation period:
- No post exposure observtaion period specified in the study report.
- Hardness:
- Not specified
- Test temperature:
- 21.8 – 23.5°C (experiment I)
21.6 – 22.8°C (experiment II) - pH:
- 7.6 - 8.4
- Dissolved oxygen:
- Not specified
- Salinity:
- Not applicable - freshwater study
- Conductivity:
- Not specified
- Nominal and measured concentrations:
- 1 / 3.2 / 10 / 32 / 100 mg/L nominal concentration
- Details on test conditions:
- Pre-Culture
Four days before the start of each test, an aliquot of the permanent culture was brought into nutrient medium and incubated under continuous lighting for 96 hours under test conditions(5000 lux, 20.6 - 23.5°C in experiment I and 21.4 – 22.4°C in experiment II). The resulting culture grew exponentially.
Before usage, the pre-culture was checked for the absence of cell aggregates and the cell number of cul-ture was determined.
Performance of the Study
For each treatment, 200 mL of the respective test item solution was mixed with the necessary amount of algal pre-culture (0.363 mL experiment I, 0.349 mL experiment II) to achieve a cell concentration of 2.3 *103 cells/mL (experiment I) and 2.0 *103 cells/mL (experiment II). For the blank control, 350 mL nutrient medium was used instead of test item solution and mixed with the necessary amount of algal pre-culture (0.635 mL experiment I, 0.610 mL experiment II).
The real cell concentration at the beginning of each test was measured with an electronic particle counter in the blank control solution. This measured value was used as start cell concentration for all replicates.
From these mixtures samples for the analytical determination were taken, afterwards the pH-value was measured.
The test vessels were filled with 45 ± 1 mL of the respective test solution and incubated open (covered with perforated plastic foil acting as a stopper) for 72 hours, shaken on an orbital shaker to keep the algae in suspension. Before the start of incubation and every 24 ± 1 hours, the cell number was determined with an electronic particle counter. After the test, samples for the analytical determination were taken, afterwards the pH value in treatments and blank control was measured again.
At the end of each test, the treatments were examined microscopically in order to assess the appearance of the algae and detect abnormalities (e.g. caused by the exposure to the test item).
The content of DOC in the test vessels was measured at the start and at the end of each experiment.
Number of replicates: 6 replicates for the blank control; 3 replicates for each treatment
Vessels: glass flasks total volume 65 mL
Duration: 72 hours
Lighting: 5000 lux
Temperature: 21.8 – 23.5°C (experiment I); 21.6 – 22.8°C (experiment II)
Replicates (Blank control): 45 ±1 mL algal medium and algae
Replicates (Treatments): 45 ±1 mL test solution and algae - Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- < 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Growth rate & Yield
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Growth rate & Yield
- Details on results:
- The study was performed using 5 concentrations ranging from 1 to 100 mg/L nominal concentration. Incubation time (test system Desmodesmus subspicatus) was 72 hours. The cell concentration of each repli-cate was determined by measuring the cell numbers every 24 ± 1 hours with an electronic particle coun-ter. Growth rate µ and the yield were determined from the cell number at the respective observation times.
In both experiments scattering inhibition values were visible and therefore no dose-response relationship. The results can therefore only be specified as a range. Statistical evaluation was performed only with the lowest treatment to demonstrate significant inhibition.
At the start and at the end of the test, the content of the test item in the test solutions was estimated by determination of the dissolved organic carbon (DOC) content in the test solutions using a carbon analyser.
The measured DOC values are all in the same very low range as the blank value. Nevertheless, the inhibition of algal growth in all treatments showed the presence of dissolved test item in the test solutions.
Therefore, the results refer to the nominal values.
The pH of the blank control should not fluctuate by more than 1.5 units. The change in the blank control was 0.1 units in experiment I and 0.6 units in experiment II, respectively.
All validity criteria were met.
No observations were made which might cause doubts concerning the validity of the study outcome. The result of the test can be considered valid. - Results with reference substance (positive control):
- The 72h-EC50 values of potassium dichromate (K2Cr2O7, CAS No. 7778-50-9) were determined in a separate reference test. The values lay within the range of the laboratory (growth rate 0.73 - 1.10 mg/L, yield 0.21 – 0.66 mg/L).
- Reported statistics and error estimates:
- Calculation of results was performed with the help of validated software (Microsoft Excel®). The estima-tion of the biological data was accomplished using the software ToxRat® Professional, version 3.2.1.
In both experiments the scattered inhibition values were apparent and therefore no dose-response rela-tionship could be established. The results can therefore only be specified as a range. Statistical evaluation was performed only with the lowest treatment (experiment II) to demonstrate significant inhibition for determination of the NOEC - Validity criteria fulfilled:
- yes
- Conclusions:
- The following results for the test item Petroleum Resins (Kendex 0897) were determined:
Results of the test item
Endpoint NOEC LOEC EC10 EC50
Growth Rate < 1 mg/L n.d. n.d. > 100 mg/L
Yield < 1 mg/L n.d. n.d. > 100 mg/L
n.d.: not determinable due to lack of dose-response relationship - Executive summary:
Determination of the toxicity of Petroleum Resins (Kendex 0897) against Desmodesmus subspicatus according to OECD 201 resp. EU C.3
Findings and Results:
Two valid experiments were performed. Due to the large scattering of the results, a second experiment was carried out in the same way as the first experiment. The results of both experiments are stated in this report. However, the biological results are only related to the second experiment.
The study was performed using 5 concentrations ranging from 1 to 100 mg/L nominal concentration. Incubation time (test system Desmodesmus subspicatus) was 72 hours. The cell concentration of each replicate was determined by measuring the cell numbers every 24 ± 1 hours with an electronic particle counter. Growth rate µ and the yield were determined from the cell number at the respective observation times.
In both experiments scattered inhibition values were visible and therefore no dose-response relationship was observed. The results can therefore only be specified as a range. Statistical evaluation was performed only with the lowest treatment to demonstrate significant inhibition.
At the start and at the end of the test, the content of the test item in the test solutions was estimated by determination of the dissolved organic carbon (DOC) content in the test solutions using a carbon analyser.
The measured DOC values are all in the same very low range as the blank value. Nevertheless, the inhibition of algal growth in all treatments showed the presence of dissolved test item in the test solutions.
Therefore, the results refer to the nominal values.
The 72h-EC50 values of potassium dichromate (K2Cr2O7, CAS No. 7778-50-9) were determined in a separate reference test. The values lay within the range of the laboratory (growth rate 0.73 - 1.10 mg/L, yield 0.21 – 0.66 mg/L).
The following results for the test item Petroleum Resins (Kendex 0897) were determined:
Results of the test item
Endpoint
NOEC
LOEC
EC10
EC50
Growth Rate
< 1 mg/L
n.d.
n.d.
> 100 mg/L
Yield
< 1 mg/L
n.d.
n.d.
> 100 mg/L
n.d.: not determinable due to lack of dose-response relationship
Reference
Biological Results of K2Cr2O7
Parameter |
Value |
95% confidence interval |
72h EC50 |
0.90 mg/L |
0.77 – 1.1 mg/L |
72h EC50 |
0.42 mg/L |
0.35 - 0.51 mg/L |
Findings
Cell Numbers
The cell numbers were determined with an electronic particle counter. The means and standard deviations of the cell numbers of the blank control and the treatments are presented in the following table:
Cell Number/mL (experiment I)
Nominal Concentration in mg/L |
Parameter |
Cell Number/mL |
|||
0 h |
24 h |
48 h |
72 h |
||
Blank control |
Mean |
2340 |
9427 |
61030 |
276953 |
Blank control |
SD |
0 |
1067 |
4114 |
15317 |
1 |
Mean |
2340 |
7047 |
50187 |
228773 |
1 |
SD |
0 |
501 |
7669 |
26534 |
3.2 |
Mean |
2340 |
7300 |
35140 |
174647 |
3.2 |
SD |
0 |
1087 |
7831 |
24831 |
10 |
Mean |
2340 |
9267 |
52027 |
221613 |
10 |
SD |
0 |
1090 |
3098 |
35878 |
32 |
Mean |
2340 |
8833 |
61193 |
276173 |
32 |
SD |
0 |
480 |
3391 |
25685 |
100 |
Mean |
2340 |
7513 |
41667 |
201140 |
100 |
SD |
0 |
477 |
6637 |
25942 |
Cell Number/mL (experiment II)
Nominal Concentration in mg/L |
Parameter |
Cell Number/mL |
|||
0 h |
24 h |
48 h |
72 h |
||
Blank control |
Mean |
2000 |
8877 |
68367 |
355470 |
Blank control |
SD |
0 |
1184 |
9391 |
46991 |
1 |
Mean |
2000 |
8813 |
48700 |
217700 |
1 |
SD |
0 |
1000 |
10122 |
33247 |
3.2 |
Mean |
2000 |
8427 |
58920 |
196393 |
3.2 |
SD |
0 |
691 |
1542 |
2278 |
10 |
Mean |
2000 |
7773 |
64507 |
236553 |
10 |
SD |
0 |
408 |
12965 |
19086 |
32 |
Mean |
2000 |
7653 |
45293 |
190827 |
32 |
SD |
0 |
945 |
8512 |
20139 |
100 |
Mean |
2000 |
8300 |
61893 |
230407 |
100 |
SD |
0 |
583 |
3551 |
12373 |
SD = Standard Deviation
Light Intensity
In the following table, the light intensity during the tests is stated:
Light Intensity (both experiments)
|
0 h |
24 h |
48 h |
72 h |
Light intensity [Lux] |
5000 |
5000 |
5000 |
5000 |
pH values
In the following table, the pH values measured at the start and the end of the tests are stated:
pH values (experiment I)
Nominal Concentration in mg/L |
0 h |
72 h |
Blank control |
7.8 |
7.7 |
1 |
7.7 |
7.7 |
3.2 |
7.7 |
7.5 |
10 |
7.6 |
7.6 |
32 |
7.7 |
7.9 |
100 |
7.7 |
7.8 |
pH values (experiment II)
Nominal Concentration in mg/L |
0 h |
72 h |
Blank control |
7.8 |
8.4 |
1 |
7.9 |
7.9 |
3.2 |
8.1 |
7.9 |
10 |
8.1 |
7.9 |
32 |
8.1 |
7.8 |
100 |
8.1 |
7.8 |
Microscopical Observations
In the following table, the appearance of the algae at the end of the tests is stated:
Microscopical Observations (both experiments)
Nominal Concentration in mg/L |
Normal and Healthy Appearance of the Algae |
Blank control |
Yes |
1 |
Yes |
3.2 |
Yes |
10 |
Yes |
32 |
Yes |
100 |
Yes |
Analytical Determination
At the start and at the end of each test, the content of the test item in the test solutions was estimated by determination of the dissolved organic carbon (DOC) content in the test solutions using a carbon analyser.
Since the measured DOC values are all in the same very low range as the blank value, the results refer to the nominal values.
The details are given in the following tables:
Measured Concentrations IC and TC (experiment I)
Nominal |
Measured TC |
Measured TC |
Measured IC |
Measured IC |
mg/L |
mg/L |
mg/L |
mg/L |
mg/L |
Blank control |
6.71 |
10.42 |
6.74 |
5.49 |
1 |
6.83 |
8.71 |
6.88 |
5.48 |
3.2 |
6.87 |
8.72 |
6.91 |
6.13 |
10 |
6.41 |
11.18 |
6.38 |
5.69 |
32 |
6.79 |
10.15 |
6.85 |
4.92 |
100 |
6.78 |
8.87 |
6.78 |
5.86 |
LOQ (Limit of quantification) TC = 4.07 mg/L
LOQ (Limit of quantification) IC = 1.34 mg/L
Measured Concentrations IC and TC (experiment II)
Nominal |
Measured TC |
Measured TC |
Measured IC |
Measured IC |
mg/L |
mg/L |
mg/L |
mg/L |
mg/L |
Blank control |
7.18 |
5.82 |
6.94 |
5.13 |
1 |
7.02 |
5.86 |
6.72 |
5.32 |
3.2 |
6.63 |
6.25 |
6.48 |
5.89 |
10 |
7.31 |
6.16 |
7.14 |
5.79 |
32 |
7.09 |
6.52 |
6.85 |
6.29 |
100 |
6.93 |
6.21 |
6.83 |
5.67 |
LOQ (Limit of quantification) TC = 2.08 mg/L
LOQ (Limit of quantification) IC = 1.27 mg/L
Measured Concentrations DOC (experiment I)
Nominal |
Measured DOC (TC-IC) |
Measured DOC (TC-IC) |
Measured DOC minus blank control |
Measured DOC minus blank control t = 72 h |
mg/L |
mg/L |
mg/L |
mg/L |
mg/L |
Blank control |
0.00 |
4.93 |
-- |
-- |
1 |
0.00 |
3.23 |
0.00 |
-1.70 |
3.2 |
0.00 |
2.59 |
0.00 |
-2.34 |
10 |
0.03 |
5.48 |
0.03 |
0.55 |
32 |
0.00 |
5.22 |
0.00 |
0.29 |
100 |
0.00 |
3.01 |
0.00 |
-1.92 |
Measured Concentrations DOC (experiment II)
Nominal |
Measured DOC (TC-IC) |
Measured DOC (TC-IC) |
Measured DOC minus blank control |
Measured DOC minus blank control t = 72 h |
mg/L |
mg/L |
mg/L |
mg/L |
mg/L |
Blank control |
0.23 |
0.68 |
-- |
-- |
1 |
0.30 |
0.54 |
0.06 |
-0.15 |
3.2 |
0.16 |
0.36 |
-0.08 |
-0.33 |
10 |
0.18 |
0.37 |
-0.06 |
-0.32 |
32 |
0.24 |
0.23 |
0.01 |
-0.46 |
100 |
0.09 |
0.54 |
-0.14 |
-0.15 |
Calculated Test item Concentrations (experiment I)
Nominal |
Calculated Concentration Test Item |
Calculated Concentration Test Item |
% of Nominal concentration |
% of Nominal concentration |
mg/L |
mg/L |
mg/L |
% |
% |
Blank control |
-- |
-- |
-- |
-- |
1 |
0.0 |
-2.0 |
0 |
-196 |
3.2 |
0.0 |
-2.7 |
0 |
-84 |
10 |
0.0 |
0.6 |
0 |
6 |
32 |
0.0 |
0.3 |
0 |
1 |
100 |
0.0 |
-2.2 |
0 |
-2 |
Calculated Test item Concentrations (experiment II)
Nominal |
Calculated Concentration Test Item |
Calculated Concentration Test Item |
% of Nominal concentration |
% of Nominal concentration |
mg/L |
mg/L |
mg/L |
% |
% |
Blank control |
-- |
-- |
-- |
-- |
1 |
0.1 |
-0.2 |
7 |
-17 |
3.2 |
-0.1 |
-0.4 |
-3 |
-12 |
10 |
-0.1 |
-0.4 |
-1 |
-4 |
32 |
0.0 |
-0.5 |
0 |
-2 |
100 |
-0.2 |
-0.2 |
0 |
0 |
Growth Rate, Yield
From the cell numbers, the Growth Rate µ and the Yield were calculated. The means and standard deviations at the end of the test are given in the following table:
Growth Rate µ, Yield (experiment I)
Nominal Concentration in mg/L |
Parameter |
Growth Rate (0-72h) [day-1] |
Yield (0-72h) |
Blank control |
Mean |
1.59 |
274613 |
SD |
0.02 |
15317 |
|
1 |
Mean |
1.53 |
226433 |
SD |
0.04 |
26534 |
|
3.2 |
Mean |
1.44 |
172307 |
SD |
0.05 |
24831 |
|
10 |
Mean |
1.51 |
219273 |
SD |
0.05 |
35878 |
|
32 |
Mean |
1.59 |
273833 |
SD |
0.03 |
25685 |
|
100 |
Mean |
1.48 |
198800 |
SD |
0.04 |
25942 |
SD = Standard Deviation
Growth Rate µ, Yield (experiment II)
Nominal Concentration in mg/L |
Parameter |
Growth Rate (0-72h) [day-1] |
Yield (0-72h) |
Blank control |
Mean |
1.72 |
353470 |
SD |
0.05 |
46991 |
|
1 |
Mean |
1.56 |
215700 |
SD |
0.05 |
33247 |
|
3.2 |
Mean |
1.53 |
194393 |
SD |
0.00 |
2278 |
|
10 |
Mean |
1.59 |
234553 |
SD |
0.03 |
19086 |
|
32 |
Mean |
1.52 |
188827 |
SD |
0.04 |
20139 |
|
100 |
Mean |
1.58 |
228407 |
SD |
0.02 |
12373 |
SD = Standard Deviation
Inhibition
The following mean inhibition values were calculated for the Growth Rate µ and the Yield.
Inhibition Values (experiment I)
Nominal concentration in mg/L |
% Inhibition |
|
Growth Rate (0-72h) |
Yield (0-72h) |
|
Blank control |
0 |
0 |
1 |
4.07 |
17.54 |
3.2 |
9.78 |
37.25 |
10 |
4.82 |
20.15 |
32 |
0.09 |
0.28 |
100 |
6.79 |
27.61 |
Inhibition Values (experiment II)
Nominal concentration in mg/L |
% Inhibition |
|
Growth Rate (0-72h) |
Yield (0-72h) |
|
Blank control |
0 |
0 |
1 |
9.48 |
38.98 |
3.2 |
11.31 |
45.00 |
10 |
7.76 |
33.64 |
32 |
11.94 |
46.58 |
100 |
8.24 |
35.38 |
Description of key information
The following results for the test item Petroleum Resins (Kendex 0897) were determined:
Results of the test item
Endpoint |
NOEC |
LOEC |
EC10 |
EC50 |
Growth Rate |
< 1 mg/L |
n.d. |
n.d. |
> 100 mg/L |
Yield |
< 1 mg/L |
n.d. |
n.d. |
> 100 mg/L |
n.d.: not determinable due to lack of dose-response relationship
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 100 mg/L
- EC10 or NOEC for freshwater algae:
- 1 mg/L
Additional information
Two valid experiments were performed. Due to the large scattering of the results, a second experiment was carried out in the same way as the first experiment. The results of both experiments are stated in this report. However, the biological results are only related to the second experiment.
The study was performed using 5 concentrations ranging from 1 to 100 mg/L nominal concentration. Incubation time (test system Desmodesmus subspicatus) was 72 hours. The cell concentration of each replicate was determined by measuring the cell numbers every 24 ± 1 hours with an electronic particle counter. Growth rate µ and the yield were determined from the cell number at the respective observation times.
In both experiments scattered inhibition values were visible and therefore no dose-response relationship was observed. The results can therefore only be specified as a range. Statistical evaluation was performed only with the lowest treatment to demonstrate significant inhibition.
At the start and at the end of the test, the content of the test item in the test solutions was estimated by determination of the dissolved organic carbon (DOC) content in the test solutions using a carbon analyser.
The measured DOC values are all in the same very low range as the blank value. Nevertheless, the inhibition of algal growth in all treatments showed the presence of dissolved test item in the test solutions.
Therefore, the results refer to the nominal values.
The following results for the test item Petroleum Resins (Kendex 0897) were determined:
Results of the test item
Endpoint |
NOEC |
LOEC |
EC10 |
EC50 |
Growth Rate |
< 1 mg/L |
n.d. |
n.d. |
> 100 mg/L |
Yield |
< 1 mg/L |
n.d. |
n.d. |
> 100 mg/L |
n.d.: not determinable due to lack of dose-response relationship
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