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EC number: 240-344-0 | CAS number: 16215-49-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 28 September - 5 October 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: 1233236-01
- Expiration date of the lot/batch: 15-12-2017
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: storage in original container, keep away from heat and direct sun irradiation
- Stability under test conditions: instable
- Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- Activated sludge from the municipal wastewater treatment plant Staufener Bucht was used as a test system. The treatment plant has a capacity of 140,000 inhabitant equivalents and clarifies predominantly domestic wastewater. The sampling date of the activated sludge was 4 October 2017. The activated sludge was kept aerated until use at test temperature. The activated sludge was washed twice by settling sludge, decanting the supernatant and re-suspending the sludge in chlorine-free tap water. The dry solid content of the activated sludge was determines as 4.5 mg/L by weight measurements before and after drying at 105 °C (mean of triplicate measurements). Before using, the activated sludge was diluted to 3 g/L dry solids with clorine-free tap water to obtain a final concentration of 1.5 g/L solids in the test.
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 3 h
- Test temperature:
- 20.4-20.7 °C
- pH:
- 7.3
- Dissolved oxygen:
- 4.99-7.63 mg/l
- Nominal and measured concentrations:
- 100mg/L test material nominal
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Breakers à 1000 ml, Schott, Mainz, Germany
- Type (delete if not applicable): Open
- Fill volume: 500 mL for set-up
- Test materials:
- Membrane pump for aeration connected with glass pipes with PolyEthylene (PE)-tubes
- Graduated cylinder 1000 ml, Schott, Mainz, Germany
- Graduated cylinder 250 ml, Schott, Mainz, Germany
- Graduated cylinder 50 ml, Schott, Mainz, Germany
- Graduated pipette 20, Brand, Wertheim, Germany
- Adjustable pipettes, 1000 µl and 5 mL, Eppendrof, Wesseling, Berzdorf, Germany
- Oxygen electrode and meter Multi 9430, WTW, Weilhem, Germany
- 250 - 300 ml BOD bottles with adapter for oxygen electrode, Germany
- pH electrode and pH meter pH 330, WTW, Weilheim, Germany
- Scale BP 221 S, Sartorius, Göttingen, Germany
- Min/Max.-thermometer
- Sludge concentration (weight of dry solids per volume): 1.5 g/L
TEST MEDIUM / WATER PARAMETERS
Chlorine-free tap water was used.
SYNTHETIC SEWAGE FEED
16 g peptone
11 g meat extract
3 g urea
0.7 g NaCl
0.4 g CaCl2 * 2 H2O
0.2 g MgSo4 * 7 H2O
2.8 g K2HPO4
Dissolved in demineralised water and made up to 1 litre.
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Oxygen consumption/ Respiration rate
TEST CONCENTRATIONS
Limit test - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Duration:
- 3 h
- Dose descriptor:
- LOEC
- Effect conc.:
- > 100 mg/L
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- Relevant effect levels: EC50 9.24 mg/L - Reported statistics and error estimates:
- The EC50 calculation was performed with the ToxRat software by probit analysis using linear max. likelihood regression (ToxRat Standard Version 2.09, © ToxRat Solutions GmbH). The EC50 was calculated from the slope of the curve.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The inhibition observed in the test vessels with 100 mg/L test concentration was between -42 and 25 % but the mean respiration rates of the test vessels were not significantly lower than the respiration rate of the controls (Student's t-test).
EC50 > 100 mg/L
NOEC ≥ 100 mg/L
PEROXAN NBC-50 had no toxic effect on the total respiration rate of activated sludge micro-organism at the concentration of 100 mg/L after the exposure period of 3 hours. - Executive summary:
The study procedure was based on OECD Guideline No. 209 (July 22nd, 2010) and performed under GLP.
A limit test with the concentration of 100 mg/L was conducted. The mean respiration rate in the treated test vessels was 26.4 mg oxygen consumption per gram sludge per hour. The mean respiration rate in the blank control was 24.5 mg/g*h. Hence, the mean respiration rate in the treated test vessels was even slightly higher than in the blank controls. This shows that the test item had no toxic effect on the total respiration rate of activated sludge microorganism at the concentration of 100 mg/L after the exposure period of 3 hours. Additionally the result of the student-t test showed no significant difference between the treated vessels abd the blank control.
Since all validity criteria of the test were met, this study is considered to be valid.
EC50(3 h, nominal) > 100 mg/L
LOEC > 100 mg/L
NOEC (3 h, nominal) ≥ 100 mg/L
Reference
Table 1: Respiration rate of activated sludge after application of the test item
No. |
Vessel |
O2 concentration [mg/L] |
consumption |
consumption |
inhibition |
|
first measurement |
last measurement |
|||||
1 |
Blank 1 |
7.04 |
5.19 |
44.4 |
29.6 |
|
2 |
Blank 2 |
7.43 |
5.64 |
43 |
28.6 |
|
3 |
Blank 3 |
7.86 |
6.85 |
24.2 |
16.2 |
|
17 |
Blank 4 |
7.39 |
5.89 |
36 |
24 |
|
18 |
Blank 5 |
7.43 |
5.93 |
36 |
24 |
|
19 |
Blank 6 |
8.17 |
6.63 |
37 |
24.6 |
|
12 |
test item 1 (100 mg/L) |
6.35 |
4.47 |
45.1 |
30.1 |
-23 |
13 |
test item 2 (100 mg/L) |
7.63 |
6.3 |
31.9 |
21.3 |
13 |
14 |
test item 3 (100 mg/L) |
4.99 |
2.82 |
52.1 |
34.7 |
-42 |
15 |
test item 4 (100 mg/L) |
5.34 |
3.62 |
41.3 |
27.5 |
-12 |
16 |
test item 5 (100 mg/L) |
7.62 |
6.47 |
27.6 |
18.4 |
25 |
coefficient of variation of blanks: |
0.18 |
|||||
mean consumption rate blanks: |
24.5 |
|||||
mean consumption rate test vessels: |
26.4 |
Description of key information
An activated sludge respiration inhibition test conducted according to OECD guideline 209 and under GLP showed no toxic effect on microorganisms up to 100 mg/L.
As the test substance has peroxide content of 50.6 %, the NOEC for the peroxide itself is 50.6 mg/L.
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 50.6 mg/L
Additional information
To investigate potential harmful effects of the test item on microorganisms, an activated sludge respiration inhibition test was performed with domestic sewage sludge according to the OECD guideline 209 and under GLP. The inhibition observed in the test vessels with 100 mg/L test concentration was -42 and 25 % but the mean respiration rates of the test vessels were not significantly lower than the respiration rate of the controls (Student's t-test). Therefore, an EC50 of > 100 mg/L and a NOEC of ≥ 100 mg/L were determined.
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