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EC number: 268-130-2 | CAS number: 68003-46-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 28 Jan 2016
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
- Version / remarks:
- 22 Jul 2010
- Deviations:
- yes
- Remarks:
- The temperature in the first experiment was 21.4 - 22.7 °C instead of 18.0 - 22.0 °C. This deviation was stated as uncritical, as normal respiration activity of the control could be observed.
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Version / remarks:
- 30 May 2008
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Landesamt für Umwelt, Wasserwirtschaft und Gewerbeaufsicht, Rheinland Pfalz, Germany
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: For the first and second experiment, a stock solution of an appropriate concentration in deionised water was prepared. Then, the stock solution was used to prepare the treatments. For the third experiment the test item, an aqueous solution was added directly with a pipette based on a density of 1.0 ± 0.1 g/mL (stated in the MSDS).
- Controls: 2 replicates before and two after measuring positive control and test item, respectively.
- Evidence of undissolved material: The solubility of the test item is sufficient. - Test organisms (species):
- activated sludge
- Details on inoculum:
- - Name and location of sewage treatment plant where inoculum was collected: The sludge was taken from the activation basin of ESN (Stadtentsorgung Neustadt) sewage treatment plant in NW-Lachen-Speyerdorf, Germany.
- Preparation of inoculum for exposure: Upon arrival in the test facility, the sludge was filtered, washed with tap water three times and re-suspended in tap water.
- Pretreatment: The activated sludge was aerated until use in the test and fed daily with 50 mL synthetic sewage feed/L.
- Initial biomass concentration: 3.14 g suspended solids/L (experiment 1), 3.18 g suspended solids/L (experiment 2), 2.84 g suspended solids/L (experiment 3) - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Hardness:
- 1.03 mmol/L (dilution water)
- Test temperature:
- 21.4 - 22.7 °C (1st experiment)
18.4 - 19.9 °C (2nd experiment)
18.1 - 20.3 °C (3rd experiment) - pH:
- 7.3 - 7.5 (1st experiment)
7.2 - 7.6 (2nd experiment)
7.2 - 7.7 (3rd experiment) - Conductivity:
- 242 µS/cm (25 °C, dilution water)
- Nominal and measured concentrations:
- Control, 3.4, 34, 345, and 3448, mg/L (nominal, 1st experiment)
Control, 2.2, 6.8, 22, 68, and 220 mg/L (nominal, 2nd experiment)
Control, 345, 759, 1586, 3448, and 7586 mg/L (nominal, 3rd experiment) - Details on test conditions:
- TEST SYSTEM
- Test vessel: 2000 mL Schott-flasks were used as test vessels (1st experiment). Glass beakers were used as test vessels (2nd and 3rd experiments). Narrow-neck glass bottles with flat bottoms (250 mL) were used as measuring flasks.
- Type: The respiration rates of samples of activated sludge fed with synthetic sewage were mesured in an enclosed cell.
- Material, size, headspace, fill volume: Material: glass; Fill volume: 500 mL
- Aeration: Purified air, using Pasteur pipettes (2nd and 3rd experiment).
- No. of vessels per concentration (replicates): 5 replicates/treatment in all three experiments, except in the 1st experiment at the treatment levels 3.4, 34, and 345 mg/L.
- No. of vessels per control (replicates): 2 replicates
- Sludge concentration (weight of dry solids per volume): 3.14 g suspended solids/L (1st experiment), 3.18 g suspended solids/L (2nd experiment), 2.84 g suspended solids/L (3rd experiment)
- Weight of dry solids per volume of reaction mixture per unit of time: 1.57 g suspended solids/L (1st experiment), 1.59 g suspended solids/L (2nd experiment), 1.42 g suspended solids/L (3rd experiment)
- Nutrients provided for bacteria: Synthetic sewage.
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Tap water.
OTHER TEST CONDITIONS
- Other: The test vessels were prepared by first adding nutrient solution and dilution water and then by adding inoculum solution in 5 minute intervals.
- Details on termination of incubation: After 3 h, the content of the 1st test vessel was poured into a 250 mL narrow-neck bottle and the respiration rate was determined by measurement of the O2-concentration over a period of max. 5 min. The following vessels were measured likewise, in 5 min intervals. The respiration rates of samples of activated sludge fed with synthetic sewage were measured in an enclosed cell containing an oxygen electrode.
EFFECT PARAMETERS MEASURED:
- Oxygen consumption: Continuously during 5 min.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 10
- Range finding study: Yes, 1st experiment.
- Test concentrations: 3.4, 34, 345, and 3448 mg/L
- Results used to determine the conditions for the definitive study: Yes, significant inhibition was observed in the 1st experiment. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 68 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks on result:
- other:
- Remarks:
- corresponding to 20 mg/L active ingredient ammonium lauroyl sarcosinate
- Details on results:
- Significant inhibition was observed in the 1st experiment.
In the second experiment´, significant inhibition was only observed in the highest treatment.
The third experiment showed a good correlation of the inhibitory effects of the test item. - Reported statistics and error estimates:
- For the treatments with the test item concentrations 220 and 68 mg/L, it was tested whether the differences between treatment and control were significant. For this determination, the values of the O2 consumption were used.
EQUALITY OF VARIANCE
In order to select a suitable test for significance, it was checked whether equality of variance was given. The calculated value F is compared with the F-test table. If the calculated value is smaller than the tabular value, equality of variance is given.
If equality of variance is given, the t-test is used; else, the WEIR test its used.
T-TEST
With the t-test, it was checked whether the differences are significant. Significance is given if the calculated t-value is bigger than the limit of significance (t-value taken from the table with degree of freedom: n1 + n2 - 2, level of significance: 95%).
WEIR TEST
If the calculated value t is greater than 2.0, significance is given (error probability 5%).
EC10, EC50
For the calculation of the EC10 and EC50, the percentage inhibition was plotted versus concentration in a Gauss-logarithmic diagram. EC10 and EC50 were determined from the x values of the regression line at y = 10% and y = 50%. - Validity criteria fulfilled:
- yes
Reference
BIOLOGICAL RESULTS
In the first experiment, significant inhibition was observed. In the second experiment, significant inhibition was observed only in the highest treatment. Both main tests (2nd and 3rd experiments) showed good correlation of the inhibitory effects of the test item.
For the determination of the biological results, the inhibition values which were found in the second and third experiment were evaluated (Table 1, and Table 2).
Table 1. Biological Results Test Item.
Parameter |
Value |
95% confidence interval |
NOEC |
68 mg/L |
-- |
3 h – EC10 |
240 mg/L |
Not determinable |
3 h – EC50 |
2000 mg/L |
1700 – 2300 mg/L |
Table 2. Biological results based on the active ingredient of the test item (29% ammonium lauroyl sarcosinate).
Parameter |
Value |
95% confidence interval |
NOEC |
20 mg/L |
-- |
3 h – EC10 |
70 mg/L |
Not determinable |
3 h – EC50 |
580 mg/L |
490 – 607 mg/L |
Description of key information
NOEC (3 h) = 68 mg/L (nominal, 20 mg/L active ingredient, OECD 209, activated sludge)
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 68 mg/L
Additional information
One study investigating the toxicity to microorganism of Ammonium N-methyl-N-(1-oxododecyl)glycinate (CAS 68003-46-3) is available.
The study was carried out according to OECD 209 under GLP-conditions with non-adapted activated sludge as inoculum. In this static test three valid experiments were performed. In the first experiment, the test item was tested using four concentrations ranging fro 3.4 - 3448 mg/L (nominal), corresponding to 1 - 1000 mg/L active ingredient. As significant inhibition of respiration was observed, two main tests (2nd and 3rd experiments) were conducted.
In the second experiment, the test item was tested using five concentrations ranging from 2.2 – 220 mg/L (nominal), corresponding to 0.64 - 64 mg/L active ingredient. In the third test the test item was tested using five concentrations ranging from 345 - 7586 mg/L (nominal), corresponding to 100 - 2200 mg/L active ingredient. In the first experiment, significant inhibition was observed. In the second experiment, significant inhibition was observed only in the highest treatment. Therefore a third experiment was performed. Both main tests (2nd and 3rd experiments) showed good correlation of the inhibitory effects of the test item. For the determination of the effect concentrations, the inhibition values which were found in the second and third experiment were evaluated. A NOEC (3 h) of 68 mg/L (nominal), corresponding to 20 mg/L based on the active ingredient, was determined.
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