Benzoic acid, 5-[(4-aminophenyl)azo]-2-hydroxy-, reaction products with 3-[(4-amino-3-methoxyphenyl)azo]benzenesulfonic acid and carbonic dichloride, sodium salts

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

13.12.2002 - 18.07.2003

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Qualifier:

according to guideline

Guideline:

other: method No. L 45: “Acute Toxicity for Daphnia, immobilization test. Chemical formulations and waste waters”

Deviations:

no

Principles of method if other than guideline:

Young daphnia born in 24 hours are segregated to the handling container from which they entered the experiment. The sensitivity of the test species is periodically monitored by testing with the reference substance, potassium dichromate.The sample is dissolved in the dilution water at a concentration of about 1.0 g / L and during the test formed a fine precipitate that gradually settled at the bottom. The volume of the test mixture for one dilution is 50 ml.Preliminary test was carried on with 10 organisms for every concentrations and control, a limit test was carried on with 3x10 organisms and two controls. The limit test is repeated 2x. Immobilization is monitored at 24 and 48 hours. Solutions are not aerated. The temperature of the solution during the test is 20.0 ± 1.0 °C. The oxygen content and pH values are measured in each vessel at the beginning and end of the test.

GLP compliance:

no

Analytical monitoring:

no

Details on test solutions:

The stock solution of the test substance was prepared in the dilution water. 1000 mg of the test substance was weighed into 1000 mL of the dilution water for the preliminary test.The concentrations of solutions used in test were obtained by dilution of the stock solution with dilution water. The stock solution for the preliminary test: 1.0 g·L-1 The stock solution for the limit test No.1 and 2: 1.0 g·L-1

Test organisms (species):

Daphnia magna

Details on test organisms:

Daphnia magna Straus were obtained by aperiodic parthenogenesis from own laboratory breeding. Daphnia were fed by a mixture of algae.The grow-up gravid females are separated into dilution water. Young daphnia born in 24 hours are segregated to the handling container from which they entered the experiment. The sensitivity of the test species and correctness of test performance is periodically verified by testing with the reference substance, potassium dichromate.TEST ORGANISM- Common name: Daphnia Magna- Strain: Straus- Source: own laboratory breeding- Age at study initiation: young daphnia born in 24 hours- Method of breeding: aperiodic parthenogenesis- Feeding during test: noACCLIMATIONThe grow-up gravid females are separated into dilution water. Young daphnia born in 24 hours are segregated to the handling container from which they entered the experiment. - Type and amount of food: a mixture of algae- Health during acclimation: healthy

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test temperature:

20.0 ± 1.0 °CLimit tests at 0; 48 hoursLimit test No. 1: 19.5; 20.0 °CLimit test No. 1: 19.2; 20.1 °C

pH:

Limit test No. 1 at 0; 48 hours: pH of the controls: 7.98 – 7.78pH of the tested concentrations: 8.04 – 7.79Limit test No. 2 at 0; 48 hours: pH of the controls: 7.96 – 7.76pH of the tested concentrations: 7.99 – 7.81

Dissolved oxygen:

Limit test No. 1: Oxygen content in the control: 8.8 mg·L-1Oxygen content in the tested concentrations: 8.8 mg·L-1Limit test No. 2 at 0; 48 hours:Oxygen content in the control: 8.9; 8.8 mg·L-1Oxygen content in the tested concentrations: 8.9; 8.8 mg·L-1

Nominal and measured concentrations:

Preliminary test: 1000, 800, 600, 400, 200 mg/L + controlFull test 1 and 2: 3x 1000 mg/L + 2x control

Details on test conditions:

TEST SYSTEM- Test vessel: glass beakers (volume of the test solution is 50ml in each test beaker)- Type (delete if not applicable): open- Aeration: no- No. of organisms per vessel: 10- No. of vessels per concentration (replicates): 3- No. of vessels per control (replicates): 2Preliminary Test: 10 animals for every concentration + controlLimit test No.1: 3x10 animals + 2 controlsLimit test No.2: 3x10 animals + 2 controlsDilution water was prepared just before the test by admixing 2.5 mL of stored solutions A, B, C and D into 1 000 mL of deionized water.A. 117.6 g CaCl2.2H20 in 1 L of deionized waterB. 49.3 g MgSO4.7H2O in 1 L of deionized waterC. 25.9 g NaHCO3 in 1 L of deionized waterD. 2.3 g KCl in 1 L of deionized waterpH 7.8, saturated with oxygen at T= 20 - 24 °C

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

The test quality criteria

These quality criteria given in the current guideline were fulfilled.

1.      Immobilisation in the controls must not exceed 10 % at the end of the test (there were no immobilisation)

2.      The dissolved oxygen concentrations (throughout the test) should remain above 3 mg·L-1

Validity criteria fulfilled:

yes

Executive summary:

The test substance, Saturnová žluť L4G 75 (Direct Yellow 44), was tested in acute immobilisation test on Daphnia magna.

The test was performed as static.

Test result: EC50 (48h) > 1000 mg·L-1

Description of key information

48 h - EC50 > 1000.0 mg.L-1

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

1 000 mg/L