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EC number: 908-084-9 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 03 July 2017 - 06 July 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 850.5400 (Algal Toxicity, Tiers I and II) (January 2012)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Storage condition: Controlled room temperature (15-25°C, below 70 RH%)
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 50 % saturated solution (of 0.125 mg/L nominal loading rate WAF) and 0.125, 0.25, 0.5, 1.0, 2.0 and 4.0 mg/L nominal loading rates WAFs.
- Sampling method: Samples were taken in plastic tubes, acidified with pure nitric acid
(9 mL sample + 1 mL HNO3)
- Sample storage conditions before analysis:(stored under -20°C until analysis) - Vehicle:
- no
- Details on test solutions:
- The test item is a multi constituent substance and poorly soluble in water therefore a test solution was prepared using a saturated solution method (water accommodated fraction, WAF) according to the Guidance Document on Aquatic Toxicity Testing of Difficult Substances and Mixtures, OECD No. 23.
Saturated test item solutions [based on nominal loading rates of 0.125, 0.25, 0.5, 1.0 , 2.0 and 4.0 mg/L (WAFs)] were prepared individually by dispersing/dissolving the amount of test item into the test medium (OECD Medium) two days before the start of the test. These solutions were shaken for about 24 hours at approximately 30°C and then equilibrated for about 24 hours at approximately 20°C. The non-dissolved test material was removed by filtration through a fine (0.22 μm) filter to give appropriate nominal loading rates.
As the lowest concentration cannot be prepared individually with a sufficient precision, therefore it was prepared from a serial dilution of the next concentration level (0.125 mg/L WAF) to give the 50 % saturated solution of 0.125 mg/L nominal loading rate WAF (200.0 mL 0.125 mg/L WAF solution was diluted with OECD Medium up to 400 mL). - Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: Pseudokirchneriella subcapitata
ACCLIMATION
- Acclimation period: 3 days
- Culturing media and conditions: same - Test type:
- not specified
- Water media type:
- freshwater
- Remarks:
- Reconstituted algal growth medium (OECD medium, according to OECD 201) was used as dilution water for both the range finding and definitive tests.
- Limit test:
- no
- Total exposure duration:
- 72 h
- Remarks on exposure duration:
- The test was started (0 hours) by inoculation of a biomass of approximately 10^4 algal cells per mL test medium.
- Hardness:
- not specified
- Test temperature:
- 22.6 – 22.8 °C measured in the flask and between 21.9 and 23.2 °C measured within the climate chamber
- pH:
- 7.68 – 8.81
- Dissolved oxygen:
- not specified
- Salinity:
- not specified
- Conductivity:
- not specified
- Nominal and measured concentrations:
- nominal: 50 % saturated solution (of 0.125 mg/L nominal loading rate WAF) and 0.125, 0.25, 0.5, 1.0, 2.0 and 4.0 mg/L nominal loading rates WAFs.
measured: <0.18 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: flask
- No. of vessels per concentration (replicates): three replicates per test concentration and six replicates in the control group
GROWTH MEDIUM
- Standard medium used: yes
- Light intensity and quality: 8373 lux (equivalent to ~113 μE/m2/s) - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EL50
- Effect conc.:
- ca. 0.9 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat. (total fraction)
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EL50
- Effect conc.:
- ca. 0.52 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat. (total fraction)
- Basis for effect:
- cell number
- Duration:
- 72 h
- Dose descriptor:
- EL50
- Effect conc.:
- ca. 0.56 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat. (total fraction)
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- NOELR
- Effect conc.:
- ca. 0.25 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat. (total fraction)
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOELR
- Effect conc.:
- ca. 0.25 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat. (total fraction)
- Basis for effect:
- cell number
- Duration:
- 72 h
- Dose descriptor:
- NOELR
- Effect conc.:
- ca. 0.25 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat. (total fraction)
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- LOELR
- Effect conc.:
- ca. 0.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat. (total fraction)
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- LOELR
- Effect conc.:
- ca. 0.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat. (total fraction)
- Basis for effect:
- cell number
- Duration:
- 72 h
- Dose descriptor:
- LOELR
- Effect conc.:
- ca. 0.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat. (total fraction)
- Basis for effect:
- biomass
- Details on results:
- - Exponential growth in the control: yes
- Observation of abnormalities: Thin cells could be observed at the two highest concentration levels of 2.0 and 4.0 mg/L nominal loading rate WAF during the whole experiment
- Results with reference substance (positive control):
- - Results with reference substance valid: yes
The 72h ErC 50: 0.88 mg/L, (95 % confidence limits: 0.81 – 0.96 mg/L)
The 72h EyC 50: 0.54 mg/L, (95 % confidence limits: 0.50 – 0.59 mg/L)
The 72h EbC 50: 0.64 mg/L, (95 % confidence limits: 0.59 – 0.70 mg/L) - Reported statistics and error estimates:
- see above
- Validity criteria fulfilled:
- not specified
- Conclusions:
- B331 72-hour EL50 (algae) = 0.90 mg/L
With respect to the inhibitory effect of the test item, the 0-72 h average specific growth rates, areas and yield were significantly different from that of the control group in the examined concentration range of 0.5 – 4.0 mg/L nominal loading rate WAF, therefore the NOELR was determined as 0.25 mg/L nominal loading rate WAF and the LOELR was determined as 0.50 mg/L nominal loading rate WAF.
Reference
Description of key information
Pseudokirchneriella subcapitata, 72 hours:
EL50 (growth rate) = 0.90 mg/L
NOELR = 0.25 mg/L
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 0.9 mg/L
- EC10 or NOEC for freshwater algae:
- 0.25 mg/L
Additional information
An acute algal inhibition study was conducted (CiToxLAB Hungary, 2017) to assess the short-term effects of B331 on aquatic plants. The study was conducted according to OECD test guideline 201 and EC method C3, and in compliance with GLP. The test media was prepared as a water accomodated fraction (WAF) with nominal loading rates of (50% saturated solution at 0.125 mg/L loading), 0.125, 0.25, 0.5, 1.0, 2.0 and 4.0; a concurrent vehicle control group (untreated dilution water) was used.
Analysis of the test solutions in all dose levels at each sampling timepoint revealed the test material concentration to be below the limit of quantitation of 0.18 mg/L (measured for copper concent by ICP-AES; LoQ = 0.016 mg Cu/L).
With respect to the inhibitory effect of the test item, the 0-72 h average specific growth rates, areas and yield were significantly different from that of the control group in the examined concentration range of 0.5 – 4.0 mg/L nominal loading rate WAF, therefore the NOELR was determined as 0.25 mg/L nominal loading rate WAF and the LOELR was determined as 0.50 mg/L nominal loading rate
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