Registration Dossier
Registration Dossier
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EC number: 219-673-9 | CAS number: 2495-35-4
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 19 Oct 2017 - 06 Jan 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2018
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Benzyl acrylate
- EC Number:
- 219-673-9
- EC Name:
- Benzyl acrylate
- Cas Number:
- 2495-35-4
- Molecular formula:
- C10H10O2
- IUPAC Name:
- benzyl acrylate
Constituent 1
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: 1B182601
- Expiration date of the lot/batch: 06 Mar 2018
- Purity: 99.86%
- Physical state/Appearance: clear colourless liquid
- Storage Conditions: room temperature in the dark
Test organisms
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
Study design
- Test type:
- flow-through
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- 24 ±1 ºC
- pH:
- 7.6 at 0h
8.8 at 72 h - Nominal and measured concentrations:
- Nominal concentration (mg/L): 0.10, 0.32, 1.0, 3.2 and 10.0
Geometric Mean Measured Test Concentration (mg/L): 0.045, 0.15, 0.52, 1.9 and 5.9 - Details on test conditions:
- TEST SYSTEM
- Test vessel: glass conical flasks
- Type: closed
- Vessel volume: 250 mL
- Fill volume: 100mL
- Aeration:
- Type of flow-through: constantly shaken at approximately 150 rpm
- Initial cells density: 5 x 10^3 cells per mL
- Mean end cells density: 8.76 x 10^5 cells per mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
OTHER TEST CONDITIONS
- Photoperiod: constant illumination
- Light intensity and quality: apprx 7000 lux - Reference substance (positive control):
- yes
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 0.85 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- other: 95% Confidence Limits
- Effect conc.:
- > 0.72 - < 1 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- ca. 0.15 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- ca. 0.52 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 0.29 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Key result
- Duration:
- 72 h
- Dose descriptor:
- other: 95% Confidence Limits
- Effect conc.:
- > 0.25 - < 0.35 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- ca. 0.045 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Key result
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- ca. 0.15 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Exposure of Pseudokirchneriella subcapitata to the test item gave the following results based on the geometric mean measured test concentrations:
Response Variable EC 50 (mg/L) 95% Confidence Limits (mg/L) NEOC (mg/L) LOEC (mg/L)
Growth Rate 0.85 0.72 - 1.0 0.15 0.52
Yield 0.29 0.25 - 0.35 0.045 0.15
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