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EC number: 302-766-4 | CAS number: 94134-01-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 22/02/2005 - 04/05/2005
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- other: ISO 14669 (1999) Water Quality - Determination of acute lethal toxicity to marine cope
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: ISO 5667-16 (1998) Water Quality Sampling - Guidance on biotesting of samples
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Solubility and stability of the test substance in the solvent/vehicle: Dissolved at 10 000 mg/L
TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing: Dissolved in seawater
- Final preparation of a solid: Dissolved in seawater
FORM AS APPLIED IN THE TEST (if different from that of starting material) Aqueous, in solution
- Analytical monitoring:
- no
- Details on sampling:
- - Concentrations: 1000, 1800, 3200, 5600, 10 009 mg/L (nominal concentrations)
- Sampling method: Mortality within a control
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Test material dissolved in seawater
- Controls: 4 mortality controls (1 replicate each). 5 positive controls of 0.1, 0.32, 0.56, 1.0 and 1.8 mg/L (2 replicates per concentration). - Test organisms (species):
- other aquatic crustacea: Acartia tonsa
- Details on test organisms:
- TEST ORGANISM
- Common name: Acartia tonsa
ACCLIMATION
- Acclimation period:
- Acclimation conditions (same as test or not): No
- Type and amount of food: Mixed algal diet (2-4 species)
- Test type:
- static
- Water media type:
- saltwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Remarks on exposure duration:
- Measurements taken at 24 and 48 hours.
- Test temperature:
- 17.9 - 21.3 °C
- pH:
- At 0 hours, pH 8.09-8.13
At 48 hours, pH 7.99-8.13 - Dissolved oxygen:
- 94-97%
- Conductivity:
- 48.5-49.7 mS/cm.
- Nominal and measured concentrations:
- Nominal concentrations for test substance were 1000, 1800, 3200, 5600, 10 009 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: crystallising dishes
- Type (delete if not applicable) closed, soda glass watch cover
- Material, size, headspace, fill volume: borosilicate glass, 50 ml
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Natural seawater from Scapa Flow, Orkney.
- Particulate matter: Filtered seawater
- Conductivity: 48.0 - 49.9 mS/cm
- Culture medium different from test medium: Yes
- Intervals of water quality measurement: Daily
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mortality of population over time.
RANGE-FINDING STUDY
- Test concentrations: Nominal concentrations 1, 10, 100, 1000 mg/ L
- Results used to determine the conditions for the definitive study: Yes - Reference substance (positive control):
- yes
- Remarks:
- 3,5 Dichlorophenol
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- 2 675 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Key result
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 1 553.85 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Duration:
- 48 h
- Dose descriptor:
- other: LC90
- Effect conc.:
- 2 733.33 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Details on results:
- - Mortality of control: 0%
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: No
- Effect concentrations exceeding solubility of substance in test medium: No - Results with reference substance (positive control):
- - Results with reference substance valid?
Yes
- Limit test: No
- Dose-response test: Yes, 1.8, 1.0, 0.56, 0.32 and 0.1 mg/L
- ECx: LC50 - Validity criteria fulfilled:
- yes
- Conclusions:
- The test substance has a LC50 of 2675.00 mg/L for 24 hours and 1553.85 mg/L for 48 hours, an LC90 value of 2733.33 mg/L for 48 hours and a NOEC value of 1000.00 mg/L for 48 hours.
- Executive summary:
The study assessed the short-term toxicity of monoethanolamine salt of nitrilotriacetic acid to aquatic invertebrates. This study was performed in compliance with GLP and follows the guidelines of ISO 14669 (1999) Water Quality - Determination of acute lethal toxicity to marine copepods (Copepoda; Crustacea) and ISO 5667 - 16 (1998) Water Quality Sampling - Guidance on biotesting of samples.
All validity criteria were met and the test organisms used was Acartia tonsa. The rangefinding test exhibited no effect at the maximum concentration (0% proportional mortality response at 48 hours). Therefore, in the definitive test nominal concentrations of 1000, 1800, 3200, 5600, 10 000 mg/L were used with 2 replicates per concentration per time point measured (10 organisms per replicate). Four mortality controls were present (0 mg/L test material) which showed a proportional mortality response of 0% over 48 hours for 1 replicate per concentration, each with 10 -11 organisms. Five positive controls were present (0.1, 0.32, 0.56, 1.0 and 1.8 mg L-1 3,5 Dichlorophenol) with 2 replicates per concentration each with 9 - 11 organisms per replicate giving a 48 hour LC50 value of 0.51 mg/L. Monoethanolamine Salt of Nitrilotriacetic Acid exhibited a 48h LC50 value of 1553.85 mg/L (dilution series) to the marine copepod Acartia tonsa in the aqueous phase.
Reference
Description of key information
The study assessed the short-term toxicity of monoethanolamine salt of nitrilotriacetic acid to aquatic invertebrates. This study was performed in compliance with GLP and follows the guidelines of ISO 14669 (1999) Water Quality - Determination of acute lethal toxicity to marine copepods (Copepoda; Crustacea) and ISO 5667 - 16 (1998) Water Quality Sampling - Guidance on biotesting of samples.
All validity criteria were met and the test organisms used was Acartia tonsa. The rangefinding test exhibited no effect at the maximum concentration (0% proportional mortality response at 48 hours). Therefore, in the definitive test nominal concentrations of 1000, 1800, 3200, 5600, 10 000 mg/L were used with 2 replicates per concentration per time point measured (10 organisms per replicate). Four mortality controls were present (0 mg/L test material) which showed a proportional mortality response of 0% over 48 hours for 1 replicate per concentration, each with 10 -11 organisms. Five positive controls were present (0.1, 0.32, 0.56, 1.0 and 1.8 mg/L 3,5 Dichlorophenol) with 2 replicates per concentration each with 9 - 11 organisms per replicate giving a 48 hour LC50 value of 0.51 mg/L. Monoethanolamine Salt of Nitrilotriacetic Acid exhibited a 48h LC50 value of 1553.85 mg/L (dilution series) to the marine copepod Acartia tonsa in the aqueous phase.
Key value for chemical safety assessment
Marine water invertebrates
Marine water invertebrates
- Effect concentration:
- 1 553.85 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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