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EC number: 306-227-4 | CAS number: 96690-34-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 18 Jul - 10 Aug 2012
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- no analytical determination was performed
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 2004
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Hessisches Ministerium für Umwelt, Energie, Landwirtschaft und Verbraucherschutz
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: a water accommondated fraction was performed: for each test concentration the required aomunt of test item was dispersed in dilution water and was shaked at 130 rpm for 24 h at room temperature; subsequently the suspensions were filtered through glass fibre filters and eluates were used for the test
- Differential loading: 0.5, 1.0, 2.0, 4.0 and 8.0 mg/L
- Controls: yes - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Strain/clone: STRAUS (clone 5)
- Source: obtained from BASF SE, Limburgerhof, Germany; the strain originated from the Institut National de Recherche Chimique Appliquee, France in 1978 and cultivated since then at BASF using medium M4 acc. Elendt
- Feeding during test: no feeding during the test
- Food type: for cultivation: with Desmodesmus subspicatus- and/or Chlorella sp.cells
METHOD FOR PREPARATION AND COLLECTION OF EARLY INSTARS OR OTHER LIFE STAGES: daphnids are transferred into fresh medium one day before the study start in order to ensure the lest animals were less than 24 hours old. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Test temperature:
- 20.8 -21.1 °C (control)
20.7 - 21.2 °C (test item concentration) - pH:
- 8.02 - 8.70 (control)
8.03 - 8.92 (test item concentration) - Dissolved oxygen:
- 8.3 - 8.5 mg O2/L (control)
7.7 - 8.4 mg O2/L (test item concentration) - Nominal and measured concentrations:
- control, 0.5, 1.0, 2.0, 4.0, 8.0 loading of the test item (mg/L)
- Details on test conditions:
- TEST SYSTEM
Test vessel:
- Type: open
- Material, size, headspace, fill volume: 50 ml-glass beakers, 50 ml volume
- No. of organisms per vessel: 5 animals
- No. of vessels per concentration (replicates): 20
- No. of vessels per control (replicates): 20
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: test item was diluted with test water which was prepared according to OECD Guidance Document on Aquatic Toxicity Testing of Difficult Substances and Mixtures, 2000
- Culture medium different from test medium: no
- Intervals of water quality measurement: pH, temperature and oxygen content was measured at test start and test end
OTHER TEST CONDITIONS
- Photoperiod: light/dark cycle 16h of light and 8 hours of darkness
EFFECT PARAMETERS MEASURED: immobility at 24 and 48 h
VEHICLE CONTROL PERFORMED: no
RANGE-FINDING STUDY
- Test concentrations: 5.1, 10.8, and 100 mg/L
- Results used to determine the conditions for the definitive study: Based on the results of the screening /range-finding test (20% inhibition at 5 mg/L and 90% inhibition at 10 mg/L) the concentrations of the main test was performed - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
- Duration:
- 48 h
- Dose descriptor:
- EL10
- Effect conc.:
- 0.56 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat. (dissolved fraction)
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% confidentail limits
- Remarks:
- 0.01 - 0.94 mg/L
- Duration:
- 48 h
- Dose descriptor:
- other: EL20
- Effect conc.:
- 0.72 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat. (dissolved fraction)
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% confidential limits
- Remarks:
- 0.05 - 1.15 mg/L
- Duration:
- 48 h
- Dose descriptor:
- EL50
- Effect conc.:
- 1.13 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat. (dissolved fraction)
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% confidential limits
- Remarks:
- 0.44 - 2.54 mg/L
- Duration:
- 48 h
- Dose descriptor:
- NOELR
- Effect conc.:
- 0.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat. (dissolved fraction)
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- LOELR
- Effect conc.:
- 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat. (dissolved fraction)
- Basis for effect:
- mobility
- Details on results:
- - Mortality of control: no mortality was observed
- Others: Biological Results are summarized in the tables 1-3 within the section: "Any other information on results incl tables" - Results with reference substance (positive control):
- - ECx: EL50 (24 h) 1.0 mg/L
- Reported statistics and error estimates:
- The inhibition values as well as the statistical calculation were done according to the guideline using the commercial computer program ToxRat Version 2.10.
- Validity criteria fulfilled:
- yes
- Remarks:
- see detailed information in section "Any other information on results incl. tables"
Reference
Validity criteria were fulfilled:
In the control without any test item no dead animals were observed.
In the test solutions the oxygen concentration did not drop below a value of 60% of the saturation value (5 mg O2/L).
Analytical Determination:
The EC50-value was calculated on the basis of the nominal concentrations tested as being agreed with the sponsor because there is no real analytical method for the active substance which could identify the test item (UVCB substance) in the aqueous phase at very low concentrations (0.5 - 10 mg/L). Only HPLC would detect this UVCB-substance but at concentrations which are 3 or more magnitudes higher. Due to these methodical problems the sponsor decided to waive the analytical part and to choose the nominal concentrations.
Table 1: Screening Test (Range-finder)
Loading of the lest item (mg/L) (Eluates/”WAF”) |
Ration immobilized Daphnia magna [%] |
|
|
t 24 h |
t 48 h |
Control (0) |
0 [0/10] |
0 [0/10] |
5 |
10 [10/10] |
20 [2/10] |
10 |
70 [7/10] |
9 [9/10] |
100 |
100 [10/10] |
100 [10/10] |
Table 2: Biological Results Definitive test
Loading of the lest item (mg/L) |
Ratio immobilised Daphnia magna [%] |
|
(Eluates/”WAF”) |
24 h |
48 h |
Control (0) |
0 [0/20]1 |
0 [0/20] 1 |
0.5 |
0 [0/20]1 |
0 [0/20]1 |
1.0 |
40 [8/20]1 |
60 [12/20]1 |
2.0 |
60 [12/20]1 |
75 [15/20]1 |
4.0 |
65 [13/20]1 |
100 [20/20]1 |
8.0 |
65 [13/20]1 |
100 [20/20]1 |
1) Numbers in brackets give the absolute numbers of immobilised animals at eachconcentration
Table 3: Endpoints
On the Basis of the nominal Concentrations [ma EC306-227-4 /,,Additin RC 3740")/L] |
|||
|
|
t24h |
t48h |
|
EL10 |
0.81 |
n.d. |
95% -CL |
lower |
n.d. |
n.d. |
|
upper |
n.d. |
n.d. |
|
EL20 |
1.15 |
0.59 |
95% -CL |
lower |
n.d. |
n.d. |
|
upper |
n.d. |
n.d. |
|
EL50 |
2.27 |
1.15 |
95% -CL |
lower |
n.d. |
n.d. |
|
upper |
n.d. |
n.d. |
Mortality |
LOEL |
2.00 |
:s: 1.00 |
|
NOEL 1.00 |
< 1.00 |
|
n.d. not determined due to mathematical reasons (inappropriate data)
Description of key information
EL50 (48h) = 1.13 mg/L (OECD 202, Daphnia magna, nominal)
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 1.13 mg/L
Additional information
One study is available, in which the short term toxicity to invertebrates was investigated according to GLP and OECD guideline 202 (SGS Institut Fresenius GmbH, 2012) using the test organism Daphnia magna. The test was performed under static test conditions and with the test item concentrations of 0.5, 1.0, 2.0, 4.0, 8.0 mg/L (WAF, nominal loading rate). Since no analytical method was applicable the endpoints were evaluated using the nominal concentrations. The EL50 (48 h) was therefore determined to be at 1.13 mg/L based on the nominal concentrations.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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