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Diss Factsheets
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EC number: 947-375-5 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- from 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- The study is performed according to the OECD 301F guideline and under GLP compliance.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Deviations:
- no
- Principles of method if other than guideline:
- Not applicable
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- None
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: Pre-conditioning will consist in aerating sludge (in mineral medium) for 5-7 days before the test and at the test temperature.
- Laboratory culture: A filtration through a fine sieve (about 1 mm) will be performed in order to remove coarse particles. After removal of any coarse particles, the sludge will be washed by decantation in a mineral medium until sludge is considered free from excess substrate or inhibitor.
- Inoculum preconditioning: Pre-conditioning will consist in aerating sludge (in mineral medium) for 5-7 days before the test and at the test temperature. - Duration of test (contact time):
- 28 d
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST ITEM PREPARATION:
- The test item is poorly soluble in water and in order to improve the dissolution the test item was dissolved in silicone oil AR 20 up to final concentration of 1 % v/v. Around 25 mg of test item was stirred with 2.5 mL silicone oil AR 20 by magnetically stirring until completely dissolved.
Then, this solution was directly supplemented or completed into 250 mL with mineral medium and sludge directly in each test vessels
The test medium was the mineral medium, a weakly saline aqueous medium.
The test item was tested at 100 mg/L in mineral medium containing 1 % v/v of silicone oil AR 20.
TEST PRINCIPLE
A measured volume of inoculated medium containing a known concentration of the test item (100 mg/L) as the nominal sole source of Carbon, is stirred in a closed vessel at a constant temperature up to 28 days with possible extension to 60 days.
Microbial respiration consumes O2 and releases CO2 which will be absorbed by soda lime pellets present in the headspace of flasks. This produces a decrease in pressure in the flask proportional to the amount of oxygen taken up by the microbial population during biodegradation, which will be expressed as a percentage of ThOD (Theoretical Oxygen Demand).
Allowance will be made for the endogenous activity of the inoculum by running parallel blanks with inoculum but without the test item. Furthermore, in order to check the procedure, a reference item control (sodium benzoate + inoculum) and a toxicity control (sodium benzoate + inoculum + test item) will be run in parallel.
The pH of the contents will be measured at the start and the end of the experiment.
TEST CONDITIONS
- Composition of medium: Mineral medium according to OECD 301F guideline
- Continuous darkness: Yes
- Test performed in closed vessels: Yes
- The pH of the contents is measured at the start and the end of the experiment. - Reference substance:
- benzoic acid, sodium salt
- Preliminary study:
- No data
- Test performance:
- No data
- Parameter:
- % degradation (O2 consumption)
- Value:
- 83.59
- Sampling time:
- 28 d
- Details on results:
- Based on ThODNH4, the degradation rate of the test item “COCOA OLEORESIN” reached 60% within the 10-Day window
on Day 6 (61.85%) and after 28 days of incubation the biodegradation reached 83.59% (80.12% to 86.24%)
Based on ThODNO3, the degradation rate of the test item “COCOA OLEORESIN” reached 60% within the 10-Day window
on Day 9 (63.13%) and after 28 days of incubation the biodegradation reached 72.07% (69.08 to 74.36%) - Results with reference substance:
- >60% (92.28% to 95.28%)
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- Under the study conditions, the test item is considered as ready biodegradable.
- Executive summary:
The aim of this study was to determine the biodegradability of the test item «COCOA OLEORESIN», according to the OECD 301F guideline, within an experimental period of 28 days by respirometric method.
The degradation rate of the test item «COCOA OLEORESIN» reached 83.59%, following the ThODNH4 and 72.07% following ThODNO3 after 28 days of incubation.
Under conditions of OECD Guideline 301F, the test item “COCOA OLERESIN”, is considered to be readily biodegradable.
Reference
Criteria
|
Validity
|
Validity for the test
|
|
Oxygen uptake of the inoculum control in 28 days
|
< 60 mg/L
|
Yes (12.7 to 14.1 mg/L) (Silicone: 19.7 to 21.1 mg/L) |
|
Biodegradability of the reference item within 14 days |
> 60%
|
Yes (92.28 to 95.28%) |
|
Biodegradability of the toxicity control within 14 days |
> 25%
|
ThODNH4 |
ThODNO3 |
Yes(80.00 and 81.14%) |
Yes(72.79 and 73.83%) |
||
Variation between the test item replicates at 28 days |
< 20% |
Yes (3.76%) |
|
pH of the inoculum control at 28 days |
6 to 8.5 |
Yes(7.2) |
Description of key information
The registered substance is regarded as readily biodegradable.
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
- Type of water:
- freshwater
Additional information
The aim of this study was to determine the biodegradability of the test item «COCOA OLEORESIN», according to the OECD 301F guideline, within an experimental period of 28 days by respirometric method.
The degradation rate of the test item «COCOA OLEORESIN» reached 83.59%, following the ThODNH4 and 72.07% following ThODNO3 after 28 days of incubation.
Under conditions of OECD Guideline 301F, the test item “COCOA OLERESIN”, is considered to be readily biodegradable.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.