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EC number: 916-461-4 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- June from 01 to 05, 1995
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study without detailed documentation
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Version / remarks:
- 1984
- GLP compliance:
- not specified
- Analytical monitoring:
- no
- Details on test solutions:
- Test item was directly dispersed in water.
- Test organisms (species):
- activated sludge
- Details on inoculum:
- - Source: the aeration stage of Severn Trent Water PLC Sewage Treatment Plant, Belper, Derbyshire.
- Preparation of inoculum for exposure: the sample was maintained at 21 °C with continuous aeration and used on the day of collection. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 3 h
- Nominal and measured concentrations:
- Nominal 1000 mg/l
- Details on test conditions:
- TEST SYSTEM
- No. of vessels per concentration: 3 replicates.
- No. of vessels per control: 2 replicates.
- Suspended solids: 4.0 g/l, prior to use.
TEST MEDIUM / WATER PARAMETERS
- pH: the pH was 6.8
EFFECT PARAMETERS MEASURED
O2 consumption rates.
TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 1.0, 10, 100 and 1000 mg/l. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Duration:
- 3 h
- Dose descriptor:
- IC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- The variation in the percentage inhibition values observed after 3 hours contact time were considered to be due to variations in the respiration rate of the activated sewage sludge micro-organisms when exposed to the test material . The effect was not considered to affect the integrity of the study given that the difference between the control respiration rates was within the range specified in the OECD Guidelines (± 15 %) and the EC50 value for the reference material, 3,5-dichlorophenol, were within the range specified in the OECD Guidelines (5-30 mg/l).
- Results with reference substance (positive control):
- Reference Material EC50 (3h): 13 mg/l
- Conclusions:
- IC50 (3h) > 1000 mg/l (nominal)
- Executive summary:
The inhibitory effect of test item on the oxygen consumption of activated sludge microorganisms was investigated according to the OECD guideline 209. The exposure time of the test substance on the activated sludge was 3 hours. The dose-range finding test was conducted at the concentrations of 1, 10, 100 and 1000 mg/l; because no rignificant inhibition was recorded up to the highest dose, the main test was conducted at the limit concentration of 1000 mg/l.
Two out of three replicates showed an increased oxygen consumption, while the third showed an inhibition of 12 %.
The variation in the percentage inhibition values observed after 3 hours contact time were considered to be due to variations in the respiration rate of the activated sewage sludge micro-organisms when exposed to the test material . The effect was not considered to affect the integrity of the study given that the difference between the control respiration rates was within the range specified in the OECD Guidelines (± 15 %) and the EC50 value for the reference material, 3,5-dichlorophenol, were within the range specified in the OECD Guidelines (5-30 mg/l).
Conclusion
IC50 (3h) > 1000 mg/l (nominal)
Reference
MAIN TEST
Concentration (mg/l) | O2 consumption rates mg O2/l/min |
% inhibition |
Control | 0.47 | - |
Control | 0.44 | - |
Test item 1000 | 0.58 | <27> |
Test item 1000 | 0.54 | <19> |
Test item 1000 | 0.4 | 12 |
Reference substance 3.2 | 0.4 | 12 |
Reference substance 32 | 0.11 | 76 |
<increase>
Variation between controls: ± 3 %
DOSE RANGE FINDING
Concentration (mg/l) | O2 consumption rates mg O2/l/min |
% inhibition |
Control | 0.36 | - |
Control | 0.35 | - |
Test item 1 | 0.32 | 10 |
Test item 10 | 0.35 | 1 |
Test item 100 | 0.38 | <7> |
Test item 1000 | 0.34 | 4 |
Reference substance 3.2 | 0.32 | 10 |
Reference substance 32 | 0.08 | 77 |
<increase>
Variation between controls: ± 1 %
Description of key information
IC50 (3h) > 1000 mg/l (nominal)
Key value for chemical safety assessment
- EC50 for microorganisms:
- 1 000 mg/L
Additional information
The inhibitory effect of test item on the oxygen consumption of activated sludge microorganisms was investigated according to the OECD guideline 209. Two out of three replicates showed an increased oxygen consumption, while the third showed an inhibition of 12 %. The variation in the percentage inhibition values observed after 3 hours contact time were considered to be due to variations in the respiration rate of the activated sewage sludge micro-organisms when exposed to the test material. The effect was not considered to affect the integrity of the study given that the difference between the control respiration rates was within the range specified in the OECD Guidelines (± 15 %).
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