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EC number: 227-645-2 | CAS number: 5921-65-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 977
- Report date:
- 1977
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 405 (Acute Eye Irritation / Corrosion)
- Version / remarks:
- 02 October 2012
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: FDA (Fed. Reg. 28 (119), 5582, 1963
- Qualifier:
- according to guideline
- Guideline:
- other: Draize and Kelley (Drug Cosmet. Industr. 71 (1952) 36)
- GLP compliance:
- no
- Remarks:
- study performed before implementation of GLP
Test material
- Reference substance name:
- 6-nonyl-1,3,5-triazine-2,4-diamine
- EC Number:
- 227-645-2
- EC Name:
- 6-nonyl-1,3,5-triazine-2,4-diamine
- Cas Number:
- 5921-65-3
- Molecular formula:
- C12H23N5
- IUPAC Name:
- 6-nonyl-1,3,5-triazine-2,4-diamine
- Test material form:
- solid: bulk
Constituent 1
Test animals / tissue source
- Species:
- rabbit
- Strain:
- New Zealand White
- Details on test animals or tissues and environmental conditions:
- TEST ANIMALS
- Age at study initiation: adult
- Housing: caged individually and recieved no hay or other extranous material that might enter eyes
Test system
- Controls:
- not required
- Amount / concentration applied:
- TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 100 mg - Duration of treatment / exposure:
- 7 days
- Observation period (in vivo):
- 7 days
- Number of animals or in vitro replicates:
- 6
- Details on study design:
- REMOVAL OF TEST SUBSTANCE
- the eyes were not washed following instillation
SCORING SYSTEM: FDA scoring scale
TOOL USED TO ASSESS SCORE: binocular magnifying glass, confirmation of corneal damage by flourescein staining if necessary
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- cornea opacity score
- Basis:
- animal: #1, #2, #4, #5, #6
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 4
- Irritation parameter:
- cornea opacity score
- Basis:
- animal #3
- Time point:
- 24/48/72 h
- Score:
- 0.33
- Max. score:
- 4
- Reversibility:
- fully reversible within: 48 h
- Irritation parameter:
- iris score
- Basis:
- animal: #1, #2, #4, #5, #6
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 2
- Irritation parameter:
- iris score
- Basis:
- animal #3
- Time point:
- 24/48/72 h
- Score:
- 0.33
- Max. score:
- 2
- Reversibility:
- fully reversible within: 48 h
- Irritation parameter:
- conjunctivae score
- Basis:
- animal: #1, #4
- Time point:
- 24/48/72 h
- Score:
- 0.33
- Max. score:
- 3
- Reversibility:
- fully reversible within: 48 h
- Irritation parameter:
- conjunctivae score
- Basis:
- animal: #2, #5
- Time point:
- 24/48/72 h
- Score:
- 0.66
- Max. score:
- 3
- Reversibility:
- fully reversible within: 72 h
- Irritation parameter:
- conjunctivae score
- Basis:
- animal: #3, #6
- Time point:
- 24/48/72 h
- Score:
- 1
- Max. score:
- 3
- Reversibility:
- fully reversible within: 7 d
- Irritation parameter:
- chemosis score
- Basis:
- animal: #1, #2, #4
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 4
- Irritation parameter:
- chemosis score
- Basis:
- animal #3
- Time point:
- 24/48/72 h
- Score:
- 0.66
- Max. score:
- 4
- Reversibility:
- fully reversible within: 7 d
- Irritation parameter:
- chemosis score
- Basis:
- animal #5
- Time point:
- 24/48/72 h
- Score:
- 0.33
- Max. score:
- 4
- Reversibility:
- fully reversible within: 48 h
- Irritation parameter:
- chemosis score
- Basis:
- animal #6
- Time point:
- 24/48/72 h
- Score:
- 0.33
- Max. score:
- 4
- Reversibility:
- fully reversible within: 72 h
- Irritant / corrosive response data:
- 24 hours after Caprinoguanamine had been brought into the eyes very slight corneal opacity and slight iritis in one rabbit and slight conjunctivitis in all six rabbits were observed. In the course of the seven day observation period all lesions recovered completely, so that at the seventh day reading all eyes were normal again.
Any other information on results incl. tables
Individual scores awarded to the ocular lesions elicited by Caprinoguanamine:
Score time point |
Cornea (max score 4) |
Iris (max. score 2) |
Conjunctivae (max. score 3) |
Chemosis (max. score 4) |
24 hours |
0/0/1/0/0/0 |
0/0/1/0/0/0 |
1/1/1/1/1/1 |
0/0/1/0/1/0 |
48 hours |
0/0/0/0/0/0 |
0/0/0/0/0/0 |
0/1/1/0/1/1 |
0/0/0/0/0/1 |
72 hours |
0/0/0/0/0/0 |
0/0/0/0/0/0 |
0/0/1/0/0/1 |
0/0/1/0/0/0 |
7 days |
0/0/0/0/0/0 |
0/0/0/0/0/0 |
0/0/0/0/0/0 |
0/0/0/0/0/0 |
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- 24 hours after Caprinoguanamine had been brought into the eyes very slight corneal opacity and slight iritis in one rabbit and slight conjunctivitis in all six rabbits were observed. In the course of the seven day observation period all lesions recovered completely, so that at the seventh day reading all eyes were normal again.
- Executive summary:
In a primary eye irritation study similar to OECD guideline 405, 2nd October 2012, 100 mg of Caprinoguanamine was instilled into the conjunctival sac of 6 adult New Zealand white rabbits. Animals then were observed for 7 days. Irritation was scored by the FDA scoring scale. 24 hours after Caprinoguanamine had been brought into the eyes very slight corneal opacity and slight iritis in one rabbit (score 1) and slight conjunctivitis (score 1) in all six rabbits were observed. All lesions were fully reversible within the 7 day observation period.
In this study the mean grades of ocular reactions at 24, 48 and 72 h after instillation were lower than the values classified as irritant by the Directive 67/548/EEC as well as GHS Regulation EC No 1272/2008.
When administered to the eye, the test article Caprinoguanamine is classified as "non-irritant".
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