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EC number: 225-806-1 | CAS number: 5089-72-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2002-05-10 to 2002-07-18
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study but the pH range is over the recommended pH range between 6-9, the hightest pH is 9.62.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 0(Control), 0.464, 1.00, 2.15, 4.64, 10.0, 21.5, 46.4 and 100 mg/L.
- Sampling method: An aliquot of test medium from all test concentrations at the start of the test before filling the test vessels. A combined aliquot was collected from the test media from all four exposure vessels of all test concentrations at the end of the test (48 hours).
- Sample storage conditions before analysis: All samples were stored at -20ºC prior to analysis. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: A 100 mg/L stock solution was prepared by adding 100 mg of the test substance to 1 L of dilution water and stirring intensely for 30 minutes. The other test concentrations were prepared by adding appropriate volumes of the stock solution to dilution water.
- Controls: Dilution water - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Source: IRChA, France
- Age at study initiation (mean and range, SD): 6-24 hours
- Method of breeding: Laboratory stock culture
- Feeding during test: None
ACCLIMATION
- Acclimation period: At least 7 days
- Acclimation conditions (same as test or not): yes
- Type and amount of food: Unicellular algae (Scenedesmus subspicatus) and a small amount of aerated sewage.
- Feeding frequency: no data
- Health during acclimation (any mortality observed): no data - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 250 mg/l as CaCO3
- Test temperature:
- 20 +/-1ºC
- pH:
- 6.99-9.62
- Dissolved oxygen:
- ≥80% ASV
- Nominal and measured concentrations:
- Nominal concentrations: 0(Control), 0.464, 1.00, 2.15, 4.64, 10.0, 21.5, 46.4 and 100 mg/L.
Measured DOC concentrations were within+/-20% of the theoretical values at the start and end of the test. The test results are therefore presented and interpreted with reference to the nominal concentrations. - Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type: no data
- Material, size: glass, 50 mL
- Aeration: none
- Renewal rate of test solution (frequency/flow rate): static test
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 1 daphnid/10 mL
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reconstituted fresh water prepared by adding salts to demineralised water.
- Alkalinity: 0.8 mmol/L
- Ca/mg ratio: 4:1
- Culture medium different from test medium: no
- Intervals of water quality measurement: Start and end of test
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 hours light, 8 hours dark
- Light intensity:500 lux (+/-20%)
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Mobility after 24 and 48 hours
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2.2
- Range finding study: no - Reference substance (positive control):
- no
- Key result
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- but exposure is to hydrolysis products
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 23 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- but exposure is to hydrolysis products
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- but exposure is to hydrolysis products
- Basis for effect:
- mobility
- Details on results:
- - Immobilisation of control: 5% (average of two sets of controls used in the test)
- Reported statistics and error estimates:
- The 48-h EC50 value was calculated by linear regression analysis incorporating probit analysis. (Probit Program Version 1.5 , U.S. Emvironmental Protection Agency, 1992).
The 24-h EC50 value and the EC0 and NOEC values were determined directly from the raw data. - Conclusions:
- A 48-h EC50 value of 23 mg/L and a NOEC of 0.464 mg/L have been determined for the effects of the test substance on mobility of Daphnia magna based on nominal concentrations of the substance. The test substance hydrolyses in water and it is therefore likely that the test organisms were primarily exposed to the hydrolysis products of the substance.
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2002-01-23 to 2002-01-25
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: A 1.0 mg/ml stock solution was prepared by placing 2.450 ml (2.5186g based on a density of 1.028 g/ml) of aminosilane in a 3.8-litre glass jar and diluting with 2500 ml of dilution water containing 0.250 ml dimethylformamide (DMF, CAS # 68-12-2). The solution was stirred for approximately 5 minutes with a magnetic stir bar and stir plate. Each test concentration was prepared by adding the appropriate amount of the 1.0 mg/ml stock solution to an intermediate vessel and diluting to 1000 ml with dilution water. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- - Source, supplier, any pretreatment, breeding method: Springborn Smithers culture facility. Daphnids were cultured in 1.0-L glass vessels containing 0.80 L of water.
- Culture medium: Water used to culture the daphnids was be prepared in the same manner and has the same characteristics as the dilution
water.
- Feeding: Daphnids were fed a unicellular green algae, Ankistrodesmus falcatus (4 x 107 cells/mL) and YCT (yeast, cereal leaves and flaked fish food) suspension, daily, at a rate of 1 mL algae and 0.5 mL YCT solution per vessel per day.
- Test organisms: Daphnids were obtained by removing all immature daphnids from the culture vessel, thus isolating mature gravid daphnids #24 hours prior to initiating the test. Young produced by these organisms were subsequently pipetted into the test beakers.
- Age of test organisms at study initiation: < 24 hours - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- The dilution water had a total hardness and alkalinity as CaCO3 of 170 mg/l and 120 mg/l, respectively
- Test temperature:
- 20 to 21 °C
- pH:
- pH measured in the dilution water and solvent control vessels was 8.0 and 7.9 respectively, at test initiation and 7.9 and 8.0 respectively, at test termination.
- Dissolved oxygen:
- The dilution water and solvent control vessels had a measured DO concentration of 8.9 and 8.7 mg/l respectively, at test initiation and 8.2 and 8.3 mg/l respectively, at test termination.
- Salinity:
- Not applicable
- Nominal and measured concentrations:
- Nominal test concentrations: 0 (Control), 0 (Solvent control) 63, 130, 250, 500 and 1000 mg/l
- Details on test conditions:
- Test vessels: The toxicity test was conducted in 250-ml glass beakers, each containing 200 ml of test solution.
- Replication: Four replicate test vessels were established for each treatment level and a dilution water and solvent control. Twenty daphnids were
impartially selected and distributed to each concentration and the controls (five daphnids per replicate vessel).
- Aeration: No aeration was provided to the test vessels.
- Dilution water source: Fortifying well water based on the formula for hard water (U.S. EPA, 1975).
- Dilution water chemistry (hardness, alkalinity, pH, TOC, TSS, salinity, Ca/Mg ratio, Na/K ratio): The dilution water had a total hardness and alkalinity as CaCO3 of 170 mg/l and 120 mg/l, respectively, a pH of 7.8 and a specific conductivity of 500 μmhos/cm. The TOC concentration of the dilution water source was 0.60 mg/l for the month of January 2002.
- Lighting (quality, intensity, and periodicity): The test area was illuminated with Sylvania Octron® fluorescent bulbs at an intensity range of 70 to 90 footcandles at the solutions' surface. The test area received a regulated photoperiod of 16 hours of light and 8 hours of darkness. Sudden transitions from light to dark and vice versa were avoided. Light intensity was measured once during the test.
- Element (unit) basis (i.e., immobilization): Immobilization - Reference substance (positive control):
- no
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 90 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- but exposure is to hydrolysis products
- Basis for effect:
- mobility
- Remarks on result:
- other: 77-110 mg/L
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- < 63 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- but exposure is to hydrolysis products
- Basis for effect:
- mobility
- Details on results:
- - Control response; No immobilization or adverse effects were observed in daphnids exposed to the control or solvent control.
- Cumulative immobilization in the treated media: 10, 90, 100, 100 and 100% immobilization was observed among daphnids exposed to the 63, 130, 250, 500, and 1000 mg/l treatment level, respectively. - Reported statistics and error estimates:
- The 48-hour EC50 for aminosilane and daphnids was calculated using probit analysis. The NOEC was determined directly from the raw data.
- Validity criteria fulfilled:
- yes
- Conclusions:
- A 48-hour EC50 value of 90 mg/l and NOEC of <63 mg/l have been determined for the effects of the test substance on mobility of Daphnia magna. The results are expressed relative to nominal concentrations of the test substance. However the substance is subject to rapid hydrolysis and under the test conditions it is therefore likely that exposure will have been to its hydrolysis products (methanol and trisilanols).
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1995-01-17 to 1995-01-19
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- Test substance concentration determined in the stock solution used to prepare the test media.
Test substance concentration was determined in all treatments at the start and end of the test. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: A 1000 mg/l stock solution was prepared by mixing for 18 hours followed by filtration. The other treatments were prepared by dilution of the stock solution. The measured DOC concentration in the stock solution was 427 mg/l which was equivalent to 986 mg/l of the test substance.
- Controls: Dilution water - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Strain: Clone 5
- Source: Stock culture
- Age at study initiation (mean and range, SD): <24 h
- Method of breeding: Parthenogenetic reproduction
- Feeding during test: None
ACCLIMATION
- Acclimation conditions (same as test or not): yes
- Type and amount of food: sufficient to be consumed each day leaving no residue
- Feeding frequency: daily - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- The sum of the Ca2+ and Mg2+ ions was 2.5 mmol
- Test temperature:
- 20 +/-1ºC
- pH:
- 8.0 - 8.3
- Dissolved oxygen:
- 8.0 - 8.4 mg/l
- Salinity:
- not applicable
- Nominal and measured concentrations:
- Nominal: 0(Control), 20, 35, 59, 108 and 197 mg/l
Measured concentration in stock solution used to prepare test media: 986 mg/l
Measured concentration in treatments at the start of the test: 0, 26, 36, 59, 106 and 194 mg/l
Measured concentration in treatments at the end of the test: 0, 19, 33, 58, 105 and 156 mg/l
The test results are interpreted with reference to nominal concentrations. - Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type: open
- Material, size, headspace, fill volume: glass cylinder containing 10 ml of test medium
- Aeration: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Elendt M4 medium
- Intervals of water quality measurement: end of test
OTHER TEST CONDITIONS
- Photoperiod: Dark
EFFECT PARAMETERS MEASURED: Immobilization after 24 and 48 h exposure
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.7 - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 81 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- but exposure is to hydrolysis products
- Basis for effect:
- mobility
- Remarks on result:
- other: 59-108 mg/L
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 35 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- but exposure is to hydrolysis products
- Basis for effect:
- mobility
- Details on results:
- - Mortality of control: 10%
- Other adverse effects control: none reported - Results with reference substance (positive control):
- 48-h EC50: >1.0, <2.0 mg/l
- Reported statistics and error estimates:
- The EC50 and its confidence interval were determined by Probit analysis. The NOEC value was obtained directly from the raw data.
- Validity criteria fulfilled:
- yes
- Conclusions:
- A 48 hour EC50 value of 81 mg/l and a NOEC of 35 mg/l have been determined for the effects of the test substance on mobility of Daphnia magna. The results are expressed relative to nominal concentrations of the test substance. However the substance is subject to rapid hydrolysis and under the test conditions it is therefore likely that exposure will have been to its hydrolysis products (methanol and trisilanols).
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- Please refer to the attached justification for grouping of substances in IUCLID Section 13.
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 81 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- but exposure is to hydrolysis products
- Basis for effect:
- mobility
- Remarks on result:
- other:
- Remarks:
- Hüls, 1995
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 90 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- but exposure is to hydrolysis products
- Basis for effect:
- mortality
- Remarks on result:
- other: 77-110 mg/L
- Remarks:
- Springborn Smithers, 2002
Referenceopen allclose all
Table 1. Test results
Nominal test substance concentration (mg/L) | Mean percentage immobilisation after 24 hours | Mean percentage immobilisation after 48 hours |
0 (Control) | 0 | 5 |
0.464 | 0 | 0 |
1.00 | 5 | 10 |
2.15 | 20 | 30 |
4.64 | 5 | 20 |
10.0 | 10 | 25 |
21.5 | 20 | 25 |
46.4 | 15 | 85 |
100 | 45 | 85 |
Table 2. Results of analysis of test media
Nominal test substance concentration (mg/L) | Theoretical DOC concentrations (mg/L) | Actual DOC concentration after 48 hours (mg/L) |
0.464 | 0.2 | 0.5 |
1.00 | 0.5 | 0.5 |
2.15 | 1.1 | 1.3 |
4.64 | 2.3 | 2.2 |
10.0 | 5.0 | 5.4 |
21.5 | 10.7 | 9.9 |
46.4 | 23.2 | 19 |
100 | 50.0 | 53 |
Table 1. Test results
Nominal concentration (mg/l) |
Mean percentage immobilisation after 24 hours |
Mean percentage immobilisation after 48 hours |
0 (Control) |
0 |
0 |
0 (Solvent control) |
0 |
0 |
63 |
5 |
10 |
130 |
30* |
90** |
250 |
100 |
100 |
500 |
100 |
100 |
1000 |
100 |
100 |
*All surviving daphnids were observed to be lethargic
** All surviving daphnids were observed to be lethargic and swimming on the bottom of the test vessel.
Table 1. Test results
Nominal test concentration (mg/l) | Mean percentage immobilisation after 24 hours | Mean percentage immobilisation after 48 hours |
0 (Control) | 0 | 10 |
20 | 0 | 0 |
35 | 0 | 0 |
59 | 10 | 15 |
108 | 50 | 80 |
197 | 90 | 90 |
Description of key information
EC50 (48 h)= 23 mg/L (OECD 202)
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 23 mg/L
Additional information
One study is available for N-[3-(triethoxysilyl)propyl]ethylenediamine (CAS 5089-72-5) with an EC50 of 23 mg/L (2003), the pH at the concentration around the EC50 was 8.5 up to 9.6, and therefore higher than the recommended range in the guideline but the study was considered valid for assessment.
Two supporting read across from the analogue substance, N-(3-(trimethoxysilyl)propyl)ethylenediamine (CAS 1760-24-3) was deemed acceptable for assessment. A 48 h EC50 value of 81 mg/l and NOEC of 35 mg/l have been determined for the effects of the test substance on mobility of Daphnia magna (1994). The results are expressed relative to nominal concentrations of the test substance. However the substance is subject to rapid hydrolysis under the tested conditions, it is therefore likely that exposure will have been to its hydrolysis products (methanol and N-(3-(trihydroxysilyl)propyl)ethylenediamine).
The 48-h EC50 value of the second available read across study (2002) was found to be 90 mg/l.
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