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EC number: 947-727-8 | CAS number: 568591-00-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 29 Nov - 27 Dec 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2018
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
- Version / remarks:
- 17 Jul 1992
- Qualifier:
- according to guideline
- Guideline:
- other: "Method for Testing the Biodegradability of Chemical Substances by Microorganisms", Testing Methods for New Chemical Substances, No. 0331-7, Pharmaceutical and Food Safety Bureau, Ministry of Health, Labour and Welfare
- Version / remarks:
- 31 Mar 2011
- GLP compliance:
- yes
Test material
- Reference substance name:
- Reaction mass of 2-ethyl-4-methyl-1H-imidazole-1-propiononitrile and 2-ethyl-5-methyl-1H-imidazole-1-propiononitrile
- EC Number:
- 947-727-8
- Cas Number:
- 568591-00-4
- Molecular formula:
- C9H13N3
- IUPAC Name:
- Reaction mass of 2-ethyl-4-methyl-1H-imidazole-1-propiononitrile and 2-ethyl-5-methyl-1H-imidazole-1-propiononitrile
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- mixture of sewage, soil and natural water
- Remarks:
- Sampled from surface water, surface soil of rivers, lake and inland sea and from return sludge of sewage plants
- Details on inoculum:
- - Source of inoculum/activated sludge: Activated sludge was sampled at 10 locations in Japan from surface water and surface soil of rivers, lake and inland sea and from return sludge of sewage plants.
- Storage length: Sampling period: September 2017; Initiation of use: 20 Oct 2017. The activated sludge was cultivated for 19.5 h after feeding with synthetic sewage.
- Pretreatment: Activated sludge was fed with synthetic sewage, which contained glucose, peptone and potassium dihydrogenphosphate dissolved in purified water (pH 7.0 ± 1.0)
- Concentration of sludge: 3830 mg/L
- Initial cell/biomass concentration: 30 mg suspended solids/L (in test vessels) - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimationopen allclose all
- Parameter followed for biodegradation estimation:
- O2 consumption
- Parameter followed for biodegradation estimation:
- DOC removal
- Parameter followed for biodegradation estimation:
- test mat. analysis
- Details on study design:
- TEST CONDITIONS
- Composition of medium: Basal culture medium
- Test temperature: 25 ± 1 °C
- pH: 7.0 - 9.0
- pH adjusted: Yes, the pH of the basal culture medium was adjusted to pH 7.0 and the pH of the test solution (sludge + test item in basal culture medium) was adjusted to pH 7.0.
- Suspended solids concentration: 30 mg suspended solids/L
- Continuous darkness: Yes
- Other: Each test solution was stirred with a stirrer.
TEST SYSTEM
- Culturing apparatus: Glass vessels and temperature controlled bath with measuring unit
- Number of culture flasks/concentration: 3
- Measuring equipment: Closed system oxygen consumption measuring apparatus (No. CM-068)
- Details of CO2 trap: Soda lime No. 1 for absorption of CO2 (Wako Pure Chemical Industries)
SAMPLING
- Sampling frequency: During the incubation period, the appearance of the test solutions was observed once a day. BOD was measured continuously. The incubation temperature was measured once a day. At the end of incubation, dissolved organic carbon (DOC) and test item concentrations were determined.
- Sampling method: 40 mL were sampled from the test solutions, which were then centrifuged to obtain supernatants for analytics.
CONTROL AND BLANK SYSTEM
- Negative control: 1 replicate: 100 mg/L test item in water (3 mL of 10.0 g/L aqueous solution of test item was added to 297 mL purified water)
- Test solution: 3 replicates: 100 mg/L test item in basal culture medium + activated sludge (3 mL of 10.0 g/L aqueous solution of test item + 2.35 mL activated sludge + 294.65 mL basal culture medium)
- Procedure/Positive control: 1 replicate: 100 mg/L aniline in basal culture medium + activated sludge (29.5 µL/30 mg aniline + 2.35 mL activated sludge + 297.65 mL basal culture medium)
- Blank control: 1 replicate: 2.35 mL activated sludge in 297.65 mL basal culture medium
Reference substance
- Reference substance:
- aniline
Results and discussion
% Degradationopen allclose all
- Parameter:
- % degradation (O2 consumption)
- Value:
- 0
- Sampling time:
- 28 d
- Remarks on result:
- other:
- Remarks:
- The calculated average value was -4%
- Parameter:
- % degradation (DOC removal)
- Value:
- 0
- Sampling time:
- 28 d
- Remarks on result:
- other:
- Remarks:
- The calculated average value was -4%
- Parameter:
- % degradation (test mat. analysis)
- Value:
- 0
- Sampling time:
- 28 d
- Details on results:
- The average percent biodegradation after 28 d was 0% by BOD, DOC and by HPLC.
The growth of the sludge was not observed at the end of cultivation.
Any other information on results incl. tables
VALIDITY CRITERIA
The test fulfilled the validity criteria of the guideline (Table 1).
Table 1. Validity criteria for OECD 301C
Criterion from the guideline |
Outcome |
Validity criterion fulfilled |
The oxygen uptake of the inoculum blank is normally 20-30 mg O2/L and should not be greater than 60 mg O2/L in 28 days. |
BOD (blank control) after 28 d: 32 mg/L |
Yes |
A test is considered valid if the difference of extremes of replicate values of the removal of the test substance at the plateau or at the end of the test, as appropriate is less than 20%. |
Difference between extremes of replicate values of % biodegradation by BOD: 2% by DOC: 3% by HPLC: 1% |
Yes |
Percentage degradation of aniline calculated from the oxygen consumption exceeds 40% after 7 days and 65% after 14 days. |
After 7 d: 68% After 14 d: 86% |
Yes |
RESULTS
Visual inspection of the test vessels, summarized in Table 2.
Table 2. Apperance of test solutions
|
Test solution |
Visual appearance |
At the start of incubation |
water + test item |
The test item was dissolved. The test solution was colorless. |
sludge + test item |
The test item was dissolved. The test solution was colorless. |
|
blank control |
Insoluble compound except the sludge was not observed. The test solution was colorless. |
|
At the end of incubation |
water + test item |
Insoluble compound was not observed. The test solution was colorless. |
sludge + test ite, |
Insoluble compound was not observed. Growth of sludge was not observed. Test solution was colorless. |
|
blank control |
Insoluble compound except the sludge was not observed. Test solution was colorless. |
ANALTICAL RESULTS
Analytical results of the test solutions after 28 d are summarized in Table 3.
Table 3. Analytical results.
|
water + test item |
sludge + test item |
theoretical amount |
|||
replicate 1 |
replicate 2 |
replicate 3 |
||||
BOD* |
mg |
0.0 |
-2.3 |
-4.2 |
-2.5 |
89.7 |
Residual amount and % residue of DOC |
mgC |
19.8 |
20.6 |
20.8 |
20.2 |
19.9 |
% |
100 |
104 |
105 |
102 |
-- |
|
Residual amount** and % residue of test item (HPLC) |
mg |
29.2 |
29.0 |
29.1 |
29.2 |
30.0 |
% |
97 |
97 |
97 |
97 |
-- |
* The value of the test solution (blank control) was subtracted from the values of the test solutions (sludge + test item)
** Calculated based on the total peak area of the chromatograms
BIOLOGICAL RESULTS
Percent biodegradation is summarized in Table 4. The % degradation of the reference control (sludge + aniline) was 68% after 7 d, 86% after 14 d, 89% after 21 d and 88% after 28 d.
Table 4. % biodegradation after 28 d.
|
sludge + test item |
|||
replicate 1 |
replicate 2 |
replicate 3 |
average |
|
Percent biodegradation by BOD |
-3 |
-5 |
-3 |
0 (-4)* |
Percent biodegradation by DOC |
-4 |
-5 |
-2 |
0 (-4)* |
Percent biodegradation of test item by HPLC |
1 |
0 |
0 |
0 |
* The average % biodegradation was regarded as 0% because the calculated average value, indicated in parentheses, was negative.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- For further details please refer to “Any other information on results incl. tables”.
- Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
- The substance is not readily biodegradable according to OECD criteria.
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