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Short-term toxicity to aquatic invertebrates

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Description of key information

The acute toxicity of Diurea 8 (in base grease) to the freshwater invertebrate Daphnia magna gave a 48-Hour EL50 (effect loading rate) value of greater than 100 mg/L WAF. The No Observed Effect Loading Rate (NOELR) was 100 mg/L WAF.

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A study was performed to assess the acute toxicity of Diurea 8 (in base grease) to Daphnia magna (Harlan 2011). The method followed was designed to be compatible with the OECD guideline 202, "Daphnia sp, Acute Immobilisation Test". Following a preliminary range-finding test, 20 daphnids (4 replicates of 5 animals) were exposed to a Water Accommodated Fraction (WAF) of Diurea 8, at a single nominal loading rate of 100 mg/L for 48 hours at a temperature of 21°C to 22°C under static test conditions. Immobilisation and any adverse reactions to exposure were recorded after 24 and 48 hours. Given that the toxicity cannot be attributed to a single component or a mixture of components, but to Diurea 8 as a whole, the results were based on nominal loading rates only.

The 48-Hour EL50 (effect loading rate) for Diurea 8 to Daphnia magna based on nominal loading rates was greater than 100 mg/L WAF. The No Observed Effect Loading Rate (NOELR) was 100 mg/L WAF. It was considered unnecessary and unrealistic to test at loading rates in excess of 100 mg/L.