Registration Dossier
Registration Dossier
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EC number: 270-821-9 | CAS number: 68478-46-6
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
Description of key information
The acute toxicity of the test item to fish (zebrafish) was determined according to the principles of the OECD-Guideline for Testing of Chemicals No. 203 (1992) and EU Council Regulation (EC) No. 440/2008/ Method C.1 (2008) in the present study.
The test was conducted with a nominal concentration of 1.8 mg/L under semi-static conditions with a renewal of the test media after 48 hours of the exposure. The duration of the test was 96 hours. Seven test organisms were exposed to the test concentration and the control, respectively. Water quality parameters (temperature, pH-value and oxygen-saturation) measured at 0, 24, 48, 72 and 96 hours were determined to be within the acceptable limits. The test media were clear throughout the test period.
The limit concentration of the test item and the control were analytically verified via LC-MS/MS from freshly prepared test media after 0 and 48 h and from the corresponding 48 h aged test media after 48 and 96 h.
At the start of the exposure and at renewal (48 hours) the measured concentrations were 94 and 105% of the nominal value. The measured concentrations in the old media (48 and 96 hours) were 84 and 92% of the nominal test item concentration. Therefore, all effect values were based on the nominal concentration of the test item.
In conclusion, the threshold concentration of 1.8 mg/L of the test item was found to have no lethal effects on zebrafish. All validity criteria of the test guideline were met.
Key value for chemical safety assessment
Additional information
The threshold concentration of 1.8 mg/L of the test item was found to have no lethal effects on zebrafish in the present study. In conclusion, since the measured concentrations of the test item were within ± 20% of the nominal concentration, the LC0 corresponds to the nominal concentration of 1.8 mg/L.
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