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EC number: 203-704-8 | CAS number: 109-78-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Additional information on environmental fate and behaviour
Administrative data
- Endpoint:
- additional information on environmental fate and behaviour
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Documentation sufficient for assessment
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 983
Materials and methods
Test guideline
- Guideline:
- other: no guideline followed
- Principles of method if other than guideline:
- Screening test: Degradation of nitriles by Brevibacteriumsp. R-312
- GLP compliance:
- not specified
- Type of study / information:
- preliminary study
Test material
- Reference substance name:
- 3-hydroxypropiononitrile
- EC Number:
- 203-704-8
- EC Name:
- 3-hydroxypropiononitrile
- Cas Number:
- 109-78-4
- Molecular formula:
- C3H5NO
- IUPAC Name:
- 3-hydroxypropanenitrile
- Reference substance name:
- Ethylene cyanohydrin
- IUPAC Name:
- Ethylene cyanohydrin
- Details on test material:
- - Name of test material (as cited in study report): Nitrile waste liquor
- Substance type: Aliphatic nitriles
- Physical state: liquid
- Composition of test material, percentage of components: Hydroxypropionitril (20%)
Succinonitrile (25%)
Monocyanopropylamine (5%)
Methylglutaronitrile (45%)
Adiponitrile (5%)
- Storage condition of test material: Nitrile waste liquor was contained in tightly capped bottles, stored refrigerated in the dark.
Constituent 1
Constituent 2
Results and discussion
Any other information on results incl. tables
In the presence of Brevibacterium sp. R-312, a bacterium, Ethylene cyanohydrin present in the waste stream from the adiponitrile process disappears. Disappearance was most effective as waste liquid concentrations of less than two percent (v/v) and at pH of between 7 and 8 (optimum pH for the degradation of various nitriles). Four concentrations of nitrile waste solution were tested, ranging from 0.5 to 2.0 %. Approximate Ethylene cyanohydrin content of the waste solution was 20 %. Ethylene cyanohydrin was degraded to less than one percent of the original in 2 hours at the 0.5 % level. Degradation was also significant up to 1.5 % level. At 2.0 %, however, inhibitory effect was manifest and no degradation was observed for Ethylene cyanohydrin. It is speculated, that the decline of degradation might be due to the presence of toxic substances in the nitrile waste liquor, which reached threshold level and significantly affected acetonitrilase activity (for exemp.: heavy metals). Thus, detailed analysis of the composition of nitrile waste liquor and further research studies have to be done. Mechanism of degradation: Brevibacterium spR312 has the highest acetonitrilase acticity among their isolates [Rev. Ferment. Ind. Aliment. 36:10-16; 1981]. Due to the activity of this enzyme, aliphatic nitriles are transformed into organic acids in two steps by hydrolysis: nitril->amid-> carboxylic acid.
Applicant's summary and conclusion
- Conclusions:
- For the following test conditions (pH range from 6/ 7-8 and nitrile (3-Hydroxypropionitrile) concentration lower than 2%):
Due to the acetonitrilase activity of the brevibacterium spR312, the degradation of the aliphatic nitrile to a carboxylic acid has been quite successfull.
After incubation of the nirile waste liquor with the cell suspension of the bacterium at 30°C and for 92 hours, 90-99% degradation was
obtained for 3-Hydroxypropionitrile concentrations from 0,5-1,5% of the nitrile waste liquor.
obtained for 3-Hydroxypropionitrile concentrations from 0,5-1,5% of the nitrile waste liquor.
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