D-Glucose, reaction products with alcohols C16-18 (even numbered)

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2017-05-03 to 2017-08-09

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Reason / purpose for cross-reference:

reference to same study

Remarks:

tox control

Qualifier:

according to guideline

Guideline:

other: OECD301F

Version / remarks:

OECD301F July 1992

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

no

Details on sampling:

- Concentrations: 128.2 mg/L sodium acetate and 8.3 mg of the test item were added into the toxicity control vessel, corresponding to a concentration of 200.2 mg ThOD/L

- Sampling method: -
- Sample storage conditions before analysis: -

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
128.2 mg/L sodium acetate and 8.3 mg of the test item were added into the toxicity control vessel, corresponding to a concentration of 200.2 mg ThOD/L. The initial pH was 7.6 and was not adjusted.

Test organisms (species):

activated sludge of a predominantly domestic sewage

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure):
Activated sludge, municipal wastewater treatment plant sludge, municipal wastewater
Breisgauer Bucht, 30 mg dry solids per litre
sampling 2017-05-03

- Laboratory culture: no
- Method of cultivation: no
- Storage conditions: not mentioned
- Storage length: 1d
- Preparation of inoculum for exposure:
The activated sludge was washed twicece with tap
water by settling the sludge, decanting the supernatant and re-suspending the sludge

- Pretreatment:
- Concentration of sludge: 30 mg dry solids per litre


- Initial cell/biomass concentration:
- Water filtered: yes/no
- Type and size of filter used, if any:

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

28 d

Key result

Duration:

28 d

Dose descriptor:

IC0

Effect conc.:

50 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: inhibition of respiration of reference substance sodium acetate according to the criteria of OECD301F

Details on results:

The degradatiion in the toxiicity control reached a value higher than 25% within 4 days. According to the validity criterion of the guideline OECD 301 the test item had no toxic effect to the inoculum.
The pH in the toxicity control vessel at the end of the test was 8.6.

Results with reference substance (positive control):

The reference compound sodium acetate reached the pass level for ready biodegradability (60% ThOD within a 10-day window) within 8 days.

Degradation table

		Test item		Toxicity control	Reference item
Test item	8.3	8.4	8.4	8.3
[mg /164 ml]
Reference item [ml /164 ml]*				164	164	164	164
ThOD [mg/1]	100.2	101.4	101.4	200.2	100.0	100.0	100.0
Day			Degradation {%]
0	0	0	0	0	0	0	0
4	13.0	15.6	21.3	27.6	55.3	55.3	55.3
8	35.6	35.2	46.2	37.5	66.6	69.4	66.6
12	51.5	53.7	62.0	46.7	74.1	76.9	74.1
16	60.8	65.6	68.4	55.7	75.1	77.6	77.6
20	65.5	70.3	70.3	62.4	73.8	79.8	71.3
24	72.1	73.7	73.7	72.8	74.8	83.8	74.8
28	71.2	75.8	75.8	77.8	76.8	82.8	73.8

Validity criteria fulfilled:

yes

Conclusions:

In a test on readily biodegradablility according to OECD301F, the test substance has no inhibitory effect on the degradation of sodium acetate at a concentration of 50mg/l.

Description of key information

In a test on readily biodegradablility according to OECD301F, the test substance has no inhibitory effect on the degradation of sodium acetate at a concentration of 50mg/l.

Key value for chemical safety assessment

EC10 or NOEC for microorganisms:

50 mg/L