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EC number: 230-256-0 | CAS number: 6990-06-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Remarks:
- + activated sludge nitrification inhibition testing (combined)
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- July 1994
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- ISO 9509 (Toxicity test for assessing the inhibition of nitrification of activated sludge microorganisms)
- Version / remarks:
- ISO DIS 9509 (1988)
- Deviations:
- not specified
- Qualifier:
- according to guideline
- Guideline:
- other: DS 298, (1984)
- Version / remarks:
- DS 298, (1984)
- Deviations:
- not specified
- GLP compliance:
- no
- Specific details on test material used for the study:
- Test material is sodium salt of fusidic acid.
The degree of dissociation of fusidic acid can be calculated as a function of pH (α = Ka/(Ka+[H+]) with the pKa for fusidic acid determined to be 4.9:
pH α
6.00 0.93
6.50 0.98
7.00 0.99
7.50 1.00
8.00 1.00
For environmental realistic conditions at pH 7-8, fusidic acid will be almost completely (99-100%) dissociated and will be present in the fusidate form. Due to the dissociation of the substance, it is therefore justified to use environmental data on the fusidate form to describe fusidic acid under these conditions. - Analytical monitoring:
- yes
- Vehicle:
- no
- Details on test solutions:
- A stock solution of the test substance was prepared in tapwater.
The following concentrations were applied: 0.05, 1.0, 10, 50, 200 mg/L for testing of the inhibition of nitrification of activated sludge from Avedøre Kloakværk A/S.
The following concentrations were applied: 1.0, 10, 50, 200, 500 mg/L for testing of both the inhibition of nitrification and respiration of activated sludge from Frederikssund vandværk and for testing inhibition of respiration of activated sludge from Avedøre Kloakværk A/S. - Test organisms (species):
- activated sludge
- Details on inoculum:
- Activated sludge from: Avedøre Kloakværk A/S and Frederikssund vandværk
- Test type:
- not specified
- Total exposure duration:
- 3 h
- Post exposure observation period:
- No
- Hardness:
- NA
- Test temperature:
- NA
- pH:
- NA
- Dissolved oxygen:
- 6 mg/L (nitrification)
- Salinity:
- NA
- Conductivity:
- NA
- Nominal and measured concentrations:
- Applied concentrations: control (no test substance), 0.05, 1, 10, 50, 200 and 500 mg/L
- Details on test conditions:
- Inhibition of nitrification (ISO/DIS 9505)
Deviations to the guideline were:
- Tap water is applied (with an alkalinity of 6 meq/L) for the nitrification of the added NH4 (25-30 mg NH4-N/L)
- Samples are taken after 10, 90 and 180 min. for determination of NO3-NO2-N and nitrification velocityis determined based on these measurements.
- During the study the test solution is aerated with an air-stone in order to have an oxygen concentration of 6 mg O2/L.
- The test is conducted with duplicates samples
Inhibition of nitrification is determined as the nitrification velocity in % of the control. And the concentration of waste water resulting in an inhibition of 20 % and 50 % respectively, is determined
Sludge applied for the test was recieved from the following sewage treatment plants (STP) Avedøre Kloakværk A/S and Frederikssund renseanlæg.
Inhibition of respiration (DS 298)
Sludge applied for the test was recieved from the following sewage treatment plants (STP) Avedøre Kloakværk A/S and Frederikssund renseanlæg.
Synthetic waste water was applied in order to ensure an optimum amount of organic substance for an optimum respiration.
The test is conducted with duplicates samples
Respiration rate was calculated based on the oxygen consumption during 6 minutes (measured after both 1/2 h and 6 h duration)
Respiration rate was related to the added amount of added sludge measured as suspended solids (SS).
The concentration inhibiting the respiration with 20% and 50% compared to the control (no test substance) was calculated applying the computer program Toxedo - Reference substance (positive control):
- no
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 200 mg/L
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of nitrification rate
- Remarks on result:
- other:
- Remarks:
- sludge from Avedøre kloakværk A/S
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 500 mg/L
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks on result:
- other:
- Remarks:
- Sludge from Frederikssund vandværk
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 500 mg/L
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of nitrification rate
- Remarks on result:
- other:
- Remarks:
- Sludge from Avedøre kloakværk A/S
- Details on results:
- Reported EC20 values (95% confidence interval in parenthesis)
EC20 (nitrification, sludge from Avedøre Kloakværk A/S) = 28.9 mg/L (22.1-37.5 mg/L)
EC20 (respiration, sludge from Avedøre Kloakværk A/S) = 238 mg/L (126-470 mg/L)
EC20 (nitrification, sludge from Frederikssund vandværk) > 500 mg/L
EC20 (respiration, sludge from Frederikssund vandværk) ca. 500 mg/L - Results with reference substance (positive control):
- NA
- Reported statistics and error estimates:
- The computerprogram Toxedo was applied for calculating the inhibition of respiration
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The inhibition of the test substance on respiration and nitrification was investigated. The following EC20 and EC50 values were obtained (95% confidence interval in parenthesis):
EC20 (nitrification, sludge from Avedøre Kloakværk A/S) = 28.9 mg/L (22.1-37.5 mg/L)
EC20 (respiration, sludge from Avedøre Kloakværk A/S) = 238 mg/L (126-470 mg/L)
EC20 (nitrification, sludge from Frederikssund vandværk) > 500 mg/L
EC20 (respiration, sludge from Frederikssund vandværk) ca. 500 mg/L
EC50 (nitrification, sludge from Avedøre Kloakværk A/S) >200 mg/L
EC50 (respiration, sludge from Avedøre Kloakværk A/S) >500 mg/L
EC50 (nitrification, sludge from Frederikssund vandværk) > 500 mg/L
EC50 (respiration, sludge from Frederikssund vandværk) >500 mg/L - Executive summary:
The inhibition of the test substance (sodium fusidate) on respiration and nitrification was investigated according to guidelines ISO DIS 9509 (1988) and DS 298, (1984).
For environmental realistic conditions at pH 7-8, fusidic acid will be almost completely (99-100%) dissociated and will be present in the fusidate form. Due to the dissociation of the substance, it is therefore justified to use environmental data on the fusidate form to describe fusidic acid under these conditions.
The study was done applying the following fusidin concentration: control (no test substance), 0.05, 1, 10, 50, 200 and 500 mg/L (duplicates) and activated sludge from Avedøre Kloakværk A/S and Frederikssund vandværk.
The following EC20 and EC50 values were obtained:
(95% confidence interval in parenthesis)
EC20 (nitrification, sludge from Avedøre Kloakværk A/S) = 28.9 mg/L (22.1-37.5 mg/L)
EC20 (respiration, sludge from Avedøre Kloakværk A/S) = 238 mg/L (126-470 mg/L)
EC20 (nitrification, sludge from Frederikssund vandværk) > 500 mg/L
EC20 (respiration, sludge from Frederikssund vandværk) ca. 500 mg/L
EC50 (nitrification, sludge from Avedøre Kloakværk A/S) >200 mg/L
EC50 (respiration, sludge from Avedøre Kloakværk A/S) >500 mg/L
EC50 (nitrification, sludge from Frederikssund vandværk) > 500 mg/L
EC50 (respiration, sludge from Frederikssund vandværk) >500 mg/L
Reference
Description of key information
The inhibition of sodium fusidate on respiration and nitrification was investigated according to guidelines ISO DIS 9509 (1988) and DS 298, (1984).
For environmental realistic conditions at pH 7-8, fusidic acid will be almost completely (99-100%) dissociated and will be present in the fusidate form. Due to the dissociation of the substance, it is therefore justified to use environmental data on the fusidate form to describe fusidic acid under these conditions.
The study applied the following fusidin concentration: control (no test substance), 0.05, 1, 10, 50, 200 and 500 mg/L (duplicates) and activated sludge from Avedøre Kloakværk A/S and Frederikssund vandværk.
The following EC20 and EC50 values were obtained:
(95% confidence interval in parenthesis)
EC20 (nitrification, sludge from Avedøre Kloakværk A/S) = 28.9 mg/L (22.1-37.5 mg/L)
EC20 (respiration, sludge from Avedøre Kloakværk A/S) = 238 mg/L (126-470 mg/L)
EC20 (nitrification, sludge from Frederikssund vandværk) > 500 mg/L
EC20 (respiration, sludge from Frederikssund vandværk) ca. 500 mg/L
EC50 (nitrification, sludge from Avedøre Kloakværk A/S) >200 mg/L
EC50 (respiration, sludge from Avedøre Kloakværk A/S) >500 mg/L
EC50 (nitrification, sludge from Frederikssund vandværk) > 500 mg/L
EC50 (respiration, sludge from Frederikssund vandværk) >500 mg/L
Key value for chemical safety assessment
- EC50 for microorganisms:
- 500 mg/L
- EC10 or NOEC for microorganisms:
- 28.9 mg/L
Additional information
The lowest toxicity to microorganisms was determined in the study of inhibition of the nitrification of the activated sludge from Avedøre Kloakværk A/S and reported as an EC20 value of 28.9 mg/L.
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