Registration Dossier
Registration Dossier
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EC number: 240-642-0 | CAS number: 16587-71-6
Adsorption / desorption
Administrative data
Link to relevant study record(s)
- Endpoint:
- adsorption / desorption: screening
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 6 Dec 2016 to 20 Dec 2016
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))
- Version / remarks:
- January, 2001
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))
- Version / remarks:
- May, 2008
- GLP compliance:
- yes (incl. QA statement)
- Type of method:
- HPLC estimation method
- Media:
- soil
- Radiolabelling:
- no
- Test temperature:
- 30°C
- Details on study design: HPLC method:
- TEST SYSTEM
The determination was carried out by the HPLC screening method, using a procedure designed to be compatible with Method C.19 Adsorption Coefficient of Commission Regulation (EC) No 440/2008 of 30 May 2008 and Method 121 of the OECD Guidelines for Testing of Chemicals, 22 January 2001. The test system utilized a high performance liquid chromatograph. A commercially available cyanopropyl reverse phase HPLC column containing lipophilic and polar moieties was used.
PREPARATION OF SAMPLE SOLUTION
Test item (0.1949 g) was diluted to 20 mL with methanol to give 9.75 x 103 mg/L.
PREPARATION OF DEAD TIME SOLUTION
The dead time was determined by measuring the retention time of formamide (purity* 99.94%) of a 656 mg/L solution in methanol:water (55:45 v/v).
PREPARATION OF REFERENCE ITEM SOLUTIONS
Solutions of reference standards were prepared in methanol. See the table under 'Any other information on materials and methods incl. tables'.
DETERMINATION TIME
The sample, dead time and reference standard solutions were injected in duplicate using the following HPLC parameters:
- HPLC System: Agilent Technologies 1100, incorporating workstation and autosampler
- Detector type: variable wavelength (VW)
- Column: Cyano 5 µm (150 x 4.6 mm id)
- Column temperature: 30 °C
- Mobile phase: methanol:water (55:45 v/v)
- pH of mobile phase: 7.3
- Flow-rate: 1.0 mL/min
- Injection volume: 10 µL
- VW detector wavelength: 210 nm (standards)
- 282 nm (sample)
CONSTRUCTION OF CALIBRATION CURVE
A correlation of log k' versus log Koc of the reference standards was plotted using linear regression. The capacity factors (k') for the reference standards were calculated.
ADSORPTION COEFFICIENT
Initially the capacity factor of the test item was calculated. The adsorption coefficient was then calculated with reference to the calibration curve. - Key result
- Type:
- Koc
- Value:
- ca. 374 dimensionless
- Key result
- Type:
- log Koc
- Value:
- ca. 2.57 dimensionless
- Details on results (HPLC method):
- ADSORPTION COEFFICIENT OF THE SAMPLE
The retention times, capacity factor and log10 Koc value determined for the sample are shown in the table under 'Any other information on results incl. tables'. The Log10 Koc was determined to be 2.57 (Koc-value of 3.74E+02). - Validity criteria fulfilled:
- yes
- Conclusions:
- The adsorption coefficient of Orivone has been determined to be 3.74E+02 (Log10 Koc 2.57).
- Executive summary:
The adsorption coefficient of Orivone has been determined to be 3.74 x 10² (log10 Koc 2.57) using the HPLC screening method, designed to be compatible with Method 121 of the OECD Guidelines for Testing of Chemicals, 22 January 2001 Method C.19 Adsorption Coefficient of Commission Regulation (EC) No 440/2008 of 30 May 2008.
Reference
Table: The retention times, capacity factor and log10 Koc value determined for the sample
Injection |
Retention time (mins) |
Capacity Factor (k’) |
Log10 k’ |
Log10 Koc |
1 |
3.528 |
0.596 |
-0.225 |
2.57 |
2 |
3.533 |
0.598 |
-0.223 |
2.58 |
Description of key information
The adsorption coefficient of Orivone has been determined to be 3.74E+02 (log10 Koc 2.57) using the HPLC screening method, designed to be compatible with Method 121 of the OECD Guidelines for Testing of Chemicals, 22 January 2001 Method C.19 Adsorption Coefficient of Commission Regulation (EC) No 440/2008 of 30 May 2008.
Key value for chemical safety assessment
- Koc at 20 °C:
- 374
Additional information
[LogKoc: 2.57]
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