4-(trans-4-Propylcyclohexyl)-1-bromobenzene

Adsorption / desorption

Link to relevant study record(s)

Reference

Endpoint:

adsorption / desorption: screening

Type of information:

experimental study

Adequacy of study:

key study

Study period:

06.-07.04.2006

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))

Version / remarks:

2001

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))

Version / remarks:

67/548/EEC

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Type of method:

HPLC estimation method

Radiolabelling:

no

Test temperature:

40 °C

Details on study design: HPLC method:

Chromatographie Conditions
Except for the detection wavelength the chromatographic conditions were the same for the
determination of t0, the establishment of the calibration graph and the measurement of the test item.

HPLC equipment: HPLC System Agilent 1100 series: HPLC pump G 1312A, autosampler, G1313A, column oven G1316A, degaser G1322A and UVNis detector
G1315B by Agilent.

Column: Stationary phase: LiChrospher 100 CN 5μm by Duratec (17405-02)
material: stainless steel, dimensions: 250 mm x 0 4.6 mm
column temperature: 40 °C

Mobile Phase: Methanol, cleaned water 50:50 % (v/v), isocratic elution

Flow rate: 1.0 ml/min

lnjection volume: 10 μI

Detection wavelength: 210 nm (dead time marker, calibration substances)
222 nm (test item)

lnjection amounts: Dead time marker: 22.6 μg
Calibration substances: 0.63 - 1.90 μg

Testitem:0.117 μg

The dead time marker was dissolved in methanol and diluted 0.1 to 1 in the mobile phase and injected as single standards twice. The calibration substances were dissolved in methanol and diluted 0.1 to 1 in the mobile phase and injected as single standards twice. The item was dissolved in methanol and diluted 0.1 to 1 in the mobile phase and injected as single sample twice.

Key result

Sample No.:

#1

Type:

log Koc

Value:

> 4.2 dimensionless

pH:

7

Temp.:

40 °C

Key result

Sample No.:

#2

Type:

log Koc

Value:

> 4.2 dimensionless

pH:

7

Temp.:

40 °C

Individual Results

Determination of the Dead Time to

Dead time marker	tr in min 1^st. measurement	tr in min 2^nd. measurement	tr in min Mean
Formamide	3.276	3.276	3.276

 

Calibration Data - 1. Measurement

Calibration substance	1 inj tr in min	k	Log k	Log Koc
Acetanilide	4.003	0.22	-0.65	1.25
Phenol	3.905	0.19	-0.72	1.32
Atrazin	4.436	0.35	-0.45	1.81
Monuron	4.572	0.40	-0.40	1.99
Triadimenol	5.071	0.55	-0.26	2.40
Linuron	5.570	0.70	-0.15	2.59
Naphthalin	6.044	0.84	-0.07	2.75
Pyrazophos	7.011	1.14	0.06	3.65
Diclofop-methly	8.167	1.49	0.17	4.20

 

Regression:logKOc=a+bx logk

Parameters: a = 3.31

b=3.17

r2= 0.9506

 

log Koc of the Test ltem - 1.Measurement

	1 inj tr in min	k	Log k	Log Koc
Test item	21.863	5.67	0.75	5.70 >4.20 *

*: beyond log Koc value of the highest calibration substance (Diclofop-methyl)

 

Calibration Data - 2. Measurement

Calibration substance	1 inj tr in min	k	Log k	Log Koc
Acetanilide	4.002	0.22	-0.65	1.25
Phenol	3.903	0.19	-0.72	1.32
Atrazin	4.433	0.35	-0.45	1.81
Monuron	4.567	0.39	-0.40	1.99
Triadimenol	5.067	0.55	-0.26	2.40
Linuron	5.565	0.70	-0.16	2.59
Naphthalin	6.041	0.84	-0.07	2.75
Pyrazophos	7.010	1.14	0.06	3.65
Diclofop-methly	8.160	1.49	0.17	4.20

 

Regression: :logKOc=a+bx logk

Parameters: a=3.32

b=3.17

r2=0.9506

 

log Koc of the Test ltem - 2. Measurement

	1 inj tr in min	k	Log k	Log Koc
Test item	21.81	5.66	0.75	5.70 >4.20 *

 

log Koc of the Test ltem

	Log Koc
1^stmeasurement	5.70 >4.20
2^ndmeasurement	5.70 >4.20
mean	5.70 >4.20

 

Validity criteria fulfilled:

yes

Conclusions:

The test item had an absorption coefficient log Koc >4.20

Executive summary:

The log Koc value of the substance was determined according to OECD Guideline 121 and European Commission Regulation (67 /548/EEC).The HPLC method is based on the correlation between the adsorption coefficient Koc and the retention of a substance on a reversed phase column packed with a commercially available cyanopropyl solid phase containing lipophilic and polar moieties.The average retention data for test substance were 5.70 (1st measurement) and 5.70 (2nd measurement). The adsorption coefficient of the test item was found to be: log Koc = 4.20.

Key value for chemical safety assessment

Other adsorption coefficients

Type:

other: 4.20

at the temperature of:

40 °C

Additional information

The log Koc value of the substance was determined according to OECD Guideline 121 and European Commission Regulation (67 /548/EEC).The HPLC method is based on the correlation between the adsorption coefficient Koc and the retention of a substance on a reversed phase column packed with a commercially available cyanopropyl solid phase containing lipophilic and polar moieties.The average retention data for test substance were 5.70 (1st measurement) and 5.70 (2nd measurement). The adsorption coefficient of the test item was found to be: log Koc = 4.20.