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EC number: 231-968-4 | CAS number: 7782-89-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2017-01-23 to 2017-05-12
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- adopted 17th July 1992
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Version / remarks:
- 30th May 2008
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: OECD guidance document no. 23, GUIDANCE DOCUMENT ON AQUATIC TOXICITY TESTING OF DIFFICULT SUBSTANCES AND MIXTURES
- Version / remarks:
- adopted 14th December 2000
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Expiration date of the batch: 2017-03-11
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Room Temperature (20 ± 5 °C); Keep under inert gas
OTHER SPECIFICS: fine powder, white to grey - Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 4.6, 10, 22, 46, 100 mg/L
- Vehicle:
- no
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Zebrafish
- Strain: Hamilton-Buchanan
- Source: Dehner Garten Center, 67433 Neustadt, Germany
- Age at study initiation: sexually immature young fish
- Length at study initiation (mean, range): 2 ± 1 cm
ACCLIMATION
- Acclimation period: 12 days
- Acclimation conditions: same as test
- Type and amount of food during acclimation: warm water fish food , totaling about 1-2 % of body weight per day
- Feeding frequency during acclimation: three times a day
- Health during acclimation: mortality did not surpass 5 %
FEEDING DURING TEST
- 24 hours before the start of the test, the test fish were no longer fed. - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- 0.83 mmol/L
- Test temperature:
- 21.2 - 23.7 °C
- pH:
- 7.5 - 8.3
- Dissolved oxygen:
- 6.7 - 9.7 mg/L
- Conductivity:
- 228 µS/cm
- Nominal and measured concentrations:
- Nominal: 4.6, 10.0, 22.0, 46.0, 100.0 mg/L
Calculated: 4.0 - 4.4, 8.8 - 9.9, 19.1 - 21.2, 41.6 - 45.1, 93.1 - 93.8 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: aquaria
- Type: open
- Material, size, headspace, fill volume: glass, max. 10 L, fill volume 7 L
- Renewal rate of test solution: after 48 h
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 1 fish/L
TEST MEDIUM / WATER PARAMETERS
- Source of dilution water: Good quality drinking water
- Total organic carbon: < 0.5 mg/L
- Metals (mg/L): aluminium 0.011, iron < 0.005, lead < 0.001, copper < 0.001, nickel < 0.001, lead < 0.001
- Pesticides: < LOD
- Chlorine: 10 mg/L
- Ca/mg ratio: ca. 9:1
- Culture medium different from test medium: No
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 12 / 12 h
EFFECT PARAMETERS MEASURED: Observations were made every 24 hours, measuring pH and O2-concentration of the old and new test solution in each vessel and documenting mortalities or abnormal behaviour.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: ca. 2.2
- Test concentrations: Nominal: 4.6, 10.0, 22.0, 46.0, 100.0 mg/L - Reference substance (positive control):
- no
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 45 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 46 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 22 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Sublethal observations / clinical signs:
Table 1: Mortality
Nominal Conc. in mg/L
Total Number of Fish used
Number of dead Fish
24 h
48 h
72 h
96 h
Blank Control
7
0
0
0
0
4.6
7
0
0
0
0
10.0
7
0
0
0
0
22.0
7
0
0
0
0
46.0
7
2
3
3
4
100.0
7
7
7
7
7
- Validity criteria fulfilled:
- yes
- Conclusions:
- In an acute short term toxicity study according to OECD Guideline 203 in Danio rerio, a LC50 of 45 mg/L was determined.
- Executive summary:
A static freshwater toxicity test was conducted to determine the acute toxicity of lithium amide to zebrafish, Danio rerio according to OECD Guideline 203. Mean calculated concentration of lithium amide ranged from 4.0 to 93.8 mg/L and from 86 to 98 % of nominal. The pH of the test solutions was not affected by the presence of lithium amide. The pH values of all test solutions ranged from 7.9 to 8.3 at test initiation and from 7.6 to 8.0 for the remainder of the test. Mortality of the zebra fish exposed for 96 hours to lithium amide ranged from 0 % at test concentrations ≤ 22.0 mg/L to 100 % at 100.0 mg /L (nominal). No mortality occurred in the dilution water control. The 96-hour LC50 was 45.0 mg/L. The NOEC of 22.0 mg/L was based on a lack of mortality and the absence of sublethal effects at this and all lower test concentrations.
Reference
Description of key information
In an acute short term toxicity study according to OECD Guideline 203 in Danio rerio (Zebra fish), an LC50 of 45 mg/L was determined.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 45 mg/L
Additional information
A static freshwater toxicity test was conducted to determine the acute toxicity of lithium amide to zebrafish, Dario rerio according to OECD Guideline 203. Mean calculated concentration of lithium amide ranged from 4.0 to 93.8 mg /L and from 86 to 98 % of nominal. The pH of the test solutions was not affected by the presence of lithium amide. The pH values of all test solutions ranged from 7.9 to 8.3 at test initiation and from 7.6 to 8.0 for the remainder of the test. Mortality of the zebra fish exposed for 96 hours to lithium amide ranged from 0 % at test concentrations ≤ 22.0 mg/L to 100 % at 100.0 mg /L (nominal). No mortality occurred in the dilution water control. The 96-hour LC50 was 45.0 mg/L. The NOEC of 22.0 mg/L was based on a lack of mortality and the absence of sublethal effects at this and all lower test concentrations.
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