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Diss Factsheets
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EC number: 946-877-1 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: inherent biodegradability
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- From 15 January to 17 February, 1991
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with generally accepted scientific standards and described in sufficient detail
- Justification for type of information:
- The read across approach is detailed into the document attached to the IUCLID section 13.
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)
- GLP compliance:
- not specified
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Details on inoculum:
- - Inoculum: a mixed population of activated sewage sludge microorganisms.
- Source: the aeration stage of Severn Trent PLC Sewage Treatment Plant, Belper, Derbyshire.
- Preparation: the activated sludge was washed by repeatedly settling and resuspending with the test water.
- Amount: usage rate equivalent to 0.75 g dry weight/I. - Duration of test (contact time):
- 28 d
- Initial conc.:
- 255 mg/L
- Based on:
- test mat.
- Remarks:
- (=100 ppm C)
- Parameter followed for biodegradation estimation:
- DOC removal
- Details on study design:
- TEST CONDITIONS
- Test temperature: 22 ± 1 °C
- Continuous darkness: yes; the test was conducted in darkness.
- Agitation: by magnetic stirrers and compressed air via narrow bore glass tubes.
- pH: pH values did not drop below 5.3 in any culture vessel during the course of the study and were adiusted to pH 7.3 daily.
- Dissolved oxygen: dissolved oxygen levels remained at approximately 7.8 mgO2/l or above in all cultures at all times.
SAMPLING
- Sampling preparation: losses by evaporation were made good by the addition of de-ionised water immediately prior to sampling.
- Sampling amount: 20 ml samples were withdrawn from each vessel and filtered through 0.45 um disposable filters; the first 10 ml was discarted. - Reference substance:
- diethylene glycol
- Remarks:
- 220 mg/l (= 100 ppm C)
- Parameter:
- % degradation (DOC removal)
- Value:
- 18
- Sampling time:
- 28 d
- Details on results:
- Test item attained 18 % degradation within 28 days and cannot, therefore, be considered as inherently biodegradable.
- Results with reference substance:
- The Standard Substance, diethylene glycol, attained 93 % degradation within 8 days and 100 % degradation by day 27, thereby confirming the suitability of the inoculum and culture conditions.
- Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
- Test item attained 18 % degradation within 28 days and cannot, therefore, be considered as inherently biodegradable.
- Executive summary:
The biodegradability of test item, exposed to activated sludge, was investigated under aerobic static conditions. The assessment was performed in accordance with Zahn-Wellens test method.
Test item attained 18 % degradation within 28 days and cannot, therefore, be considered as inherently biodegradable.
The Standard Substance, diethylene glycol, attained 93 % degradation within 8 days and 100 % degradation by day 27, thereby confirming the suitability of the inoculum and culture conditions.
Conclusion
Based on the test results, the substance resulted to be not biodegradable under the test conditions, since less than 20 % degradation was attained after 28 days of contact time.
Reference
Details on test results
Day | DOC* value mg/l (Mean of 2 Assays) | % biodegradation | ||
Test item | Reference | Test item | Reference | |
0 Hours | 130 | 88 | 0 | 0 |
3 Hours | 130 | 88 | 0 | 0 |
1 | 130 | 91 | 0 | <3> |
2 | 132 | 94 | <2> | <6> |
3 | 132 | 93 | <2> | <5> |
6 | 131 | 74 | <1> | 17 |
8 | 126 | 6 | 3 | 93 |
10 | 130 | 5 | 0 | 95 |
14 | 116 | 2 | 11 | 97 |
16 | 117 | 1 | 10 | 99 |
21 | 115 | 3 | 11 | 96 |
23 | 114 | 3 | 12 | 96 |
27 | 108 | 0 | 17 | 100 |
28 | 107 | 0 | 18 | 100 |
<increase>
Description of key information
neither readily nor inherently biodegradable
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
- Type of water:
- freshwater
Additional information
Acid Green 025 is a diaminoanthraquinones, with external sulfate groups. The substance is not expected to be readly biodegradable because of its chemical structure and its specific function. Commonly, dyes undergo a primary transformation, i.e. discolourization due to the interrupting the conjugation; nevertheless, the degradation process involves more steps and take more time.
Because there is no experimental information about the biodegradability potential of Acid Green 025, the available information on the structural analogues Similar Substance 01 and Similar Substance 02 has been taken into consideration; the read across approach can be considered as appropriate and suitable to assess the property under investigation (details about the approach are reported into the IUCLID section 13).
The biodegradability of Similar Substance 01 was investigated under aerobic static conditions, in accordance with Zahn-Wellens test method. Test item attained 18 % degradation within 28 days and cannot, therefore, be considered as inherently biodegradable.
The Standard Substance, diethylene glycol, attained 93 % degradation within 8 days and 100 % degradation by day 27, thereby confirming the suitability of the inoculum and culture conditions.
Also the biodegradability potential of the Similar Substance 01 was investigated according to the Zahn-Wellens/EMPA Test method. Test item attined between 4 and 16 % degradation, based on DOC removal, within 28 days, thus it cannot be considered as inherently biodegradable.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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