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EC number: 241-010-7 | CAS number: 16941-12-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 4 September - 26 September 2003
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study conduced to GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 003
- Report date:
- 2004
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Hexachloroplatinic acid (hydrate)
- Cas Number:
- 26023-84-7
- Molecular formula:
- K2PtCl6
- IUPAC Name:
- Hexachloroplatinic acid (hydrate)
- Details on test material:
- - Name of test material (as cited in study report): Hexachloroplatinum(IV)-solution
- Substance type:
- Physical state: liquid
- Analytical purity:
- Impurities (identity and concentrations):
- Composition of test material, percentage of components: 25% Pt
- Isomers composition:
- Purity test date: 2003-08-08
- Lot/batch No.: 2700/76-03
- Expiration date of the lot/batch: 31 July 2004
- Stability under test conditions: not stated
- Storage condition of test material: room temperature; opaque plastic bottle
- Other:
Constituent 1
Method
- Target gene:
- Histidine (S. typhimurium) and tryptophan (E.coli) loci
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Species / strain / cell type:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 from male Sprague-Dawley rats given prior treatment with phenobarbital abd betanaphthoflavone
- Test concentrations with justification for top dose:
- Two main experiments were performed:
In experiment 1 (plate incorporation method) the test item was assayed in TA1535 at 2.5, 5.0, 10.0, 20.0, 40.0 and 80.0 ug/plate, and in TA1537, TA98, TA100 and WP2uvra at 1.25, 2.5, 5.0, 10.0, 20.0 and 40.0 ug/plate (without S9). In all five strains it was tested at 10.0, 20.0, 40.0, 80.0, 160.0 ug/plate (with S9); TA98 and TA100 were also tested at 320 ug/plate (with S9).
In experiment 2 (pre-incubation method) the test item was assayed in TA1535, TA98 and TA100 at 0.313, 0.625, 1.25, 2.5, 5.0 and 10.0 ug/plate (in the absence of S9); TA100 was also tested at 20.0 and 40.0 ug/plate. TA1537 was tested at 0.625, 1.25, 2.5, 5.0, 10.0, and 20.0 ug/plate (without S9).In the presence of S9, all four Salmonella strains were tested at 2.5, 5.0, 10.0, 20.0, 40.0 and 80.0 ug/plate; TA1537 and TA100 were also tested at 1.25 and 160 ug/plate, and TA98 additionally at 0.625 ug/plate (with S9). WP2uvra was not used in experiment 2. - Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: sterile distilled water
- Justification for choice of solvent/vehicle: soluble at 50.0 mg/ml. Since 100 ul of test item soluition used in the preparation of each plate, this permitted a max. conc. of 5000 ug/plate in toxicity test.
Controlsopen allclose all
- Untreated negative controls:
- yes
- Remarks:
- Dimethylsulphoxide (DMSO)
- Negative solvent / vehicle controls:
- yes
- Remarks:
- Sterile distilled water
- Positive controls:
- yes
- Positive control substance:
- methylmethanesulfonate
- Positive controls:
- yes
- Positive control substance:
- sodium azide
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- Positive controls:
- yes
- Positive control substance:
- 2-nitrofluorene
- Positive controls:
- yes
- Positive control substance:
- other: 2-Aminoanthracene
- Details on test system and experimental conditions:
- Salmonella typhimurium strains TA1535, TA1537, TA100, TA98 and Escherichia coli WP2uvrA were used in experiment 1 (plate incorporation method) and all four S. typhimurium strains in experiment 2 (pre-incubation method), excluding E. Coli WP2uvrA. 3 replicate plates. Dose range finding study, and two main experiments (1 and 2) only.
- Evaluation criteria:
- For the test item to be considered mutagenic, two-fold (or more) increases in mean revertant numbers must be observed at two consecutive dose-levels or at the highest practicable dose-level only. In addition, there must be evidence of a dose-response relationship showing increasing numbers of mutant colonies with increasing dose-levels.
Number of cells evaluated per dose group not given in report. - Statistics:
- Not reported
Results and discussion
Test resultsopen allclose all
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Genotoxicity:
- positive
- Remarks:
- dose-related increases, at least two fold
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with
- Genotoxicity:
- ambiguous
- Remarks:
- dose-related increase in revertant numbers (experiments 1 & 2)
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with
- Genotoxicity:
- ambiguous
- Remarks:
- dose-related increase in revertant numbers (experiments 1 & 2)
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- without
- Genotoxicity:
- negative
- Remarks:
- experiments 1 & 2
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- without
- Genotoxicity:
- negative
- Remarks:
- experiments 1 & 2
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Remarks:
- experiments 1 & 2
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Remarks:
- experiments 1 & 2
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
positive
In an OECD Test Guideline 471 study, to GLP, hexachloroplatinum(IV) solution induced reverse mutations in Salmonella typhimurium strains TA98 and TA100 and in Escherichia coli WP2 uvrA under the experimental conditions. - Executive summary:
In an OECD Test Guideline 471 study, conducted according to GLP, hexachloroplatinum(IV) solution was assessed for its ability to induce gene mutations in strains of Salmonella typhimurium (TA1535, TA1537, TA98, TA100) and Escherichia coli (WP2 uvrA). The test was performed in two independent experiments, with dose levels determined following an initial toxicity test, both in the absence and presence of metabolic activation (S9) using liver fraction from rats pre-treated with phenobarbitone and beta-naphthoflavone.
In experiment 1, dose-related increases in revertant numbers, which were at least two-fold the control values, were observed in WP2 uvrA both in the absence and presence of S9. Dose-related increases in revertant numbers were also observed in TA98 and TA100 tester strains in the presence of S9.
In experiment 2, conducted using the four S. typhimurium strains, large dose-related increases in revertant numbers were observed at higher dose levels in TA98 in the presence of S9. Dose-related and reproducible increases in revertant numbers were also observed in TA100 tester strain in the presence of S9. Although these increases did not reach two-fold the control values they were considered clear evidence of a mutagenic effect.
It was concluded that hexachloroplatinum(IV) solution was mutagenic in S. typhimurium strains TA98 and TA100 and in E. coli WP2 uvrA under the experimental conditions.
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