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EC number: 918-205-7 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Description of key information
Oral LD50 (rat, m/f): 186 mg/kg bw (OECD 401)
Dermal LD50 (rat, m/f): > 2000 mg/kg bw (OECD 402)
Key value for chemical safety assessment
Acute toxicity: via oral route
Link to relevant study records
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1984-07-18 to 1984-08-13
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- Version / remarks:
- 1984
- Deviations:
- no
- GLP compliance:
- not specified
- Test type:
- standard acute method
- Limit test:
- no
- Species:
- rat
- Strain:
- other: Tif:RAIf (SPF), F3-crosses of RII 1/TIF x RII 2/Tif
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Species: Rat, Tif:RAIf (SPF), F3-crosses of RII 1/TIF x RII 2/Tif
- Age at study initiation: approximately 7-8 weeks old
- Weight at study initiation: 167 - 209 g
- Fasting period before study: Food was withheld overnight prior to dosing.
- Housing: 5 animals/sex/cage
- Diet: ad libitum
- Water: ad libitum
- Acclimation period: no data
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 +/-3
- Humidity (%): 55 +/- 15
- Air changes (per hr): 15
- Photoperiod (hrs dark / hrs light): 12/12 - Route of administration:
- oral: gavage
- Vehicle:
- other: distilled water containing CMC (carboxymethyl cellulose) and polysorbate 80
- Details on oral exposure:
- Vehicle: Distilled water containing 0.5% carboxymethyl cellulose and 0.1% polysorbate 80 (prepared by Pharmaceutical Division, Ciba-Geigy Ltd.).
Volume (mL/kg body weight) applied: 20 at the highest dose, 10 at all other doses. - Doses:
- 100, 300, 1000 and 5000 mg/kg body weight
- No. of animals per sex per dose:
- 5
- Control animals:
- no
- Details on study design:
- Mortality/Viability: Twice daily on working days, once on weekend days
Body weights: Days 1 (pre-administration), 7, 14 and at death
Clinical signs: daily
Necropsy: Spontaneously dying animals were submitted to a group necropsy as soon as possible; survivors at the end of the observation period.
Observation period: 14 days - Statistics:
- From the body weights, the group means and their standard deviations were calculated.
Where feasible, the LD50 including the 95% confidence limit were computed by the logit method (J. Berkson, J.Am. Stat. Ass. 39. 357-65, 1944) - Key result
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- 186 mg/kg bw
- Based on:
- test mat.
- 95% CL:
- >= 84 - <= 328
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- 130 mg/kg bw
- Based on:
- test mat.
- Remarks on result:
- other: no 95% CL calculated
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- 263 mg/kg bw
- Based on:
- test mat.
- 95% CL:
- >= 87 - <= 907
- Mortality:
- See table in section "Any other information on results incl. tables"
- Clinical signs:
- other: Dyspnoea, exophthalamus, ruffed fur and curved position are symptoms commonly seen during the observation time. In addition the following symptoms were considered to be treatment related: - clonic-tonic convulsions preceding early death in the 2000 and 50
- Gross pathology:
- No treatment-related deviations from normal morphology could be detected.
- Interpretation of results:
- Category 3 based on GHS criteria
- Conclusions:
- Oral LD50 186 mg/kg bw; acute oral toxicity category 3 based on the criteria of REGULATION (EC) No 1272/2008 as well as the Globally Harmonized System of Classification and Labelling of Chemicals (GHS) of the United Nations.
Reference
The incidence of mortality
Dose level (mg/kg bw) |
Mortality males |
Mortality females |
Time of death (day(s)) |
100 |
2/5 |
1/5 |
1, 6, 9 |
300 |
4/5 |
2/5 |
1 |
1000 |
5/5 |
5/5 |
1 |
5000 |
5/5 |
5/5 |
1 |
Endpoint conclusion
- Endpoint conclusion:
- adverse effect observed
- Dose descriptor:
- LD50
- Value:
- 186 mg/kg bw
- Quality of whole database:
- Data from a guideline study with reliability 1.
Acute toxicity: via inhalation route
Endpoint conclusion
- Endpoint conclusion:
- no study available
Acute toxicity: via dermal route
Link to relevant study records
- Endpoint:
- acute toxicity: dermal
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1984-07-17 to 1984-07-31
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 402 (Acute Dermal Toxicity)
- Version / remarks:
- 1984
- Deviations:
- no
- GLP compliance:
- not specified
- Test type:
- standard acute method
- Limit test:
- yes
- Species:
- rat
- Strain:
- other: Tif:RAIf (SPF), F3-crosses of RII 1/TIF x RII 2/Tif
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Species: Rat, Tif:RAIf (SPF), F3-crosses of RII 1/TIF x RII 2/Tif
- Age at study initiation: approximately 7-8 weeks old
- Weight at study initiation: 183 - 215 g
- Fasting period before study: no
- Housing: 5 animals/sex/cage
- Diet: ad libitum
- Water: ad libitum
- Acclimation period: no data
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 +/-3
- Humidity (%): 55 +/- 15
- Air changes (per hr): 15
- Photoperiod (hrs dark / hrs light): 12/12 - Type of coverage:
- semiocclusive
- Vehicle:
- other: distilled water containing 0.5% carboxymethylcellulose and 0.1% polysorbate 80
- Details on dermal exposure:
- TEST SITE
- Area of exposure, pretreatement: Approximately 24 hours before treatment an area on the back of the rat of at least 10% of the body surface was shaved with an electric clipper.
- % coverage: no data
- Type of wrap if used: The required amount of the test substance was evenly dispersed on the skin. It was covered with a gauze lined semiocclusive dressing, which was fastened around the trunk with an adhesive elastic bandage.
REMOVAL OF TEST SUBSTANCE
- Washing (if done): yes with lukewarm water
- Time after start of exposure: 24 h
TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 2000 mg/kg bw
- Concentration (if solution): n.a.
- Constant volume or concentration used: n.a.
- For solids, paste formed: yes
VEHICLE
- Amount(s) applied (volume or weight with unit): no data, however vehicle distilled water containing 0.5% carboxymethylcellulose and 0.1% polysorbate 80 was used in an amount sufficient to form a paste only
- Duration of exposure:
- 24 h
- Doses:
- 2000 mg/kg bw
- No. of animals per sex per dose:
- 5
- Control animals:
- not required
- Details on study design:
- Mortality/Viability: Twice daily on working days, once on weekend days
Body weights: Days 1 (pre-administration), 7, 14 and at death
Clinical signs: daily
Necropsy: Spontaneously dying animals were submitted to a group necropsy as soon as possible; survivors at the end of the observation period.
Observation period: 14 days - Statistics:
- From the body weights, the group means and their standard deviations were calculated.
- Key result
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 2 000 mg/kg bw
- Based on:
- test mat.
- Remarks on result:
- no indication of skin irritation up to the relevant limit dose level
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- > 2 000 mg/kg bw
- Based on:
- test mat.
- Remarks on result:
- no indication of skin irritation up to the relevant limit dose level
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- > 2 000 mg/kg bw
- Based on:
- test mat.
- Remarks on result:
- no indication of skin irritation up to the relevant limit dose level
- Mortality:
- No animal died.
- Clinical signs:
- other: Sedation on exposure day, dyspnoe from exposure day to post-exposure day 13, exopthalmus on post-exposure days 1-4, ruffed fur from exposure day to post-exposure day 12, ventral body posture from exposure day to post-exposure day 1 and curved body posture
- Gross pathology:
- No treatment-related deviations from normal morphology could be detected.
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- Dermal LD50 > 2000 mg/kg bw; classification criteria of REGULATION (EC) No 1272/2008 as well as of the Globally Harmonized System of Classification and Labelling of Chemicals (GHS) of the United Nations no met.
Reference
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed
- Dose descriptor:
- discriminating dose
- Value:
- 2 000 mg/kg bw
- Quality of whole database:
- Data from a guideline study with reliability 1.
Additional information
In an acute oral toxicity study according to OECD guideline 401 (1984), n-Butyltriphenylphosphonium bromide was administered by oral gavage at doses of 100, 300, 1000 and 5000 mg/kg bw to five rats per sex per dose. The fasted, 7-8 weeks old rats were given a single oral dose of n-Butyltriphenylphosphonium bromide in distilled water containing 0.5% carboxymethyl cellulose end 0.1% polysorbate 80 at dose volume of 20 mL/kg body weight at the dose of 5000 mg/kg bw and 10 mL/kg bw at all other dose levels. All animals were subjected to daily observations. Body weight was recorded on days 1, 7, 14 and at death. Macroscopic examination was performed on the day of death or after terminal sacrifice (day 14). All rats treated with 1000 or 5000 mg/kg body weight were found dead within 1 hour after treatment, in the dose group 300 mg/kg bw 4/5 males and 2/5 females died within 3 hours after treatment. In the lowest dose group on male was found death within 1 hour after treatment and a further male on day 6 and one female on day 9. Dyspnoea, exophthalmus, ruffed fur and curved position are symptoms commonly seen during the observation time. In addition, a slight sedation in rats of all dose groups immediately following application, and clonic-tonic convulsions preceding early death in the 2000 and 5000 mg/kg bw dose groups were considered to be treatment related. The surviving animals showed body weight gain during the observation period and recovered within 11-14 days. No treatment-related deviations from normal morphology could be detected.
Oral LD50 (rat, m/f): 186 (95% CL 84-328) mg/kg bw.
In an acute dermal toxicity study according to OECD guideline 402 (1984), n-Butyltriphenylphosphonium bromide was administered dermally for 24 hours at a dose of 2000 mg/kg bw to five rats per sex. Approximately 24 hours before treatment an area on the back of the 7-8 weeks old rat of at least 10% of the body surface was shaved with an electric clipper. The rats were given a single dermal dose of n-Butyltriphenylphosphonium bromide moistened with distilled water containing 0.5% carboxymethyl cellulose end 0.1% polysorbate 80. The treated skin was covered with a gauze lined semiocclusive dressing, which was fastened around the trunk with an adhesive elastic bandage. After an exposure period of 24 hours the dressing was removed and the skin was cleaned with lukewarm water. All animals were subjected to daily observations. Body weight was recorded on days 1, 7, 14 and at death. Macroscopic examination was performed on the day of death or after terminal sacrifice (day 14). All rats survived until terminal sacrifice. Dyspnoea, exophthalmus, ruffed fur and curved position are symptoms commonly seen during the observation time. Except for this, neither unusual symptoms nor cutaneous changes which could be related to the treatment were observed. The animals showed body weight gain during the observation period and recovered within 14 days. No treatment-related deviations from normal morphology could be detected.
Dermal LD50(rat, m/f): > 2000 mg/kg bw.
Justification for classification or non-classification
Oral LD50 rat 186 mg/kg bw; acute oral toxicity category 3 based on the criteria of REGULATION (EC) No 1272/2008 as well as the Globally Harmonized System of Classification and Labelling of Chemicals (GHS) of the United Nations.
n-Butyltriphenylphosphonium bromide is not classified for dermal acute toxicity because dermal LD50 rat is > 2000 mg/kg bw.
n-Butyltriphenylphosphonium bromide is not classified for inhalative toxicity because of lacking data.
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